Previous
Aim:
This study aimed to identify and analyze the top 100 most cited digital health and mobile health (m-health) publications. It could aid researchers in the identification of promising new research avenues, additionally supporting the establishment of international scientific collaboration between interdisciplinary research groups with demonstrated achievements in the ar
Methods:
On 30th August, 2023, the Web of Science Core Collection (WOSCC) electronic database was queried to identify the top 100 most cited digital health papers with a comprehensive search string. From the initial search, 106 papers were identified. After screening
Results:
The top 100 papers on digital health received a total of 49,653 citations. Over half of them (n = 55) were published during 2013–2017. Among these 100 papers, 59 were original articles, 36 were reviews, 4 were editorial materials, and 1 was a proceeding paper. All papers were written in English. The University of London and the University of Cali
Conclusions:
The findings underscore key areas of focus in the field and prominent contributors, providing a roadmap
Aim:
This study aimed to identify and analyze the top 100 most cited digital health and mobile health (m-health) publications. It could aid researchers in the identification of promising new research avenues, additionally supporting the establishment of international scientific collaboration between interdisciplinary research groups with demonstrated achievements in the ar
Methods:
On 30th August, 2023, the Web of Science Core Collection (WOSCC) electronic database was queried to identify the top 100 most cited digital health papers with a comprehensive search string. From the initial search, 106 papers were identified. After screening
Results:
The top 100 papers on digital health received a total of 49,653 citations. Over half of them (n = 55) were published during 2013–2017. Among these 100 papers, 59 were original articles, 36 were reviews, 4 were editorial materials, and 1 was a proceeding paper. All papers were written in English. The University of London and the University of Cali
Conclusions:
The findings underscore key areas of focus in the field and prominent contributors, providing a roadmap
DOI: https://doi.org/10.37349/edht.2024.00013
Aim:
Aloysia citrodora has a long history of traditional use in treating various ailments. This study evaluated the in vivo chemopreventive efficacy and systemic toxicity of an extract of A. citrodora in a transgenic mouse model of HPV16 (human papillomavirus type 16)-induced cancer.
Methods:
The experiment involved six groups (n = 5): group 1 (G1, wild-type (WT), water), group 2 (G2, HPV, water), group 3 (G3, WT, 0.013 g/mL), group 4 (G4, HPV, 0.006 g/mL), group 5 (G5, HPV, 0.008 g/mL), and group 6 (G6, HPV, 0.013 g/mL). Throughout the assay, humane endpoints, body weight, food, and water consumption were recorded weekly. The internal organs and skin of the mice were collected
Results:
A. citrodora extract seems to reduce the incidence of dysplastic and in situ carcinoma skin lesions induced by HPV16 in this model, suggesting that dietary supplementation with concentrations of 0.008 g/mL and 0.013 g/mL may have beneficial chemopreventive effects.
Conclusions:
The extract did not induce any concentration-dependent toxicological effects on any of the parameters included in the study, indicating a favorable toxicological profile under these experimental conditions.
Aim:
Aloysia citrodora has a long history of traditional use in treating various ailments. This study evaluated the in vivo chemopreventive efficacy and systemic toxicity of an extract of A. citrodora in a transgenic mouse model of HPV16 (human papillomavirus type 16)-induced cancer.
Methods:
The experiment involved six groups (n = 5): group 1 (G1, wild-type (WT), water), group 2 (G2, HPV, water), group 3 (G3, WT, 0.013 g/mL), group 4 (G4, HPV, 0.006 g/mL), group 5 (G5, HPV, 0.008 g/mL), and group 6 (G6, HPV, 0.013 g/mL). Throughout the assay, humane endpoints, body weight, food, and water consumption were recorded weekly. The internal organs and skin of the mice were collected
Results:
A. citrodora extract seems to reduce the incidence of dysplastic and in situ carcinoma skin lesions induced by HPV16 in this model, suggesting that dietary supplementation with concentrations of 0.008 g/mL and 0.013 g/mL may have beneficial chemopreventive effects.
Conclusions:
The extract did not induce any concentration-dependent toxicological effects on any of the parameters included in the study, indicating a favorable toxicological profile under these experimental conditions.
DOI: https://doi.org/10.37349/emed.2024.00228
Aim:
AI research, development, and implementation are expanding at an exponential pace across healthcare. This paradigm shift in healthcare research has led to increased demands
Methods:
This study was conducted using the Delphi method by devising a survey using the Google
Results:
A total of 33 survey respondents were part of the initial Delphi process with the majority (45%) in the 36–45 years age group. The respondents were located across the USA (61%), UK (24%), and Australia (9%) as the top 3 countries, with a pre-dominant healthcare background (42%) as early-career professionals (3–10 years’ experience) (42%). Feedback showed an overall agreeable consensus (mean ranges 4.1–4.8, out of 5) as cumulative scores throughout all sections. The majority of the consensus was agreeable with the Discussion (Other) section of the checklist (median 4.8 (interquartile range (IQR) 4.8-4.8)), whereas the least agreed section was the Ground Truth (Expert(s) review) section (median 4.1 (IQR 3.9–4.2)) and the Methods (Outcomes) section (median 4.1 (IQR 4.1–4.1)) of the checklist. The final checklist after consensus and revision included a total of 8 sections and 50 questions.
Conclusions:
The HUMANE international consensus has reflected on further research on the potential of this checklist as an established consensus in improving the reliability and quality of research in this field.
Aim:
AI research, development, and implementation are expanding at an exponential pace across healthcare. This paradigm shift in healthcare research has led to increased demands
Methods:
This study was conducted using the Delphi method by devising a survey using the Google
Results:
A total of 33 survey respondents were part of the initial Delphi process with the majority (45%) in the 36–45 years age group. The respondents were located across the USA (61%), UK (24%), and Australia (9%) as the top 3 countries, with a pre-dominant healthcare background (42%) as early-career professionals (3–10 years’ experience) (42%). Feedback showed an overall agreeable consensus (mean ranges 4.1–4.8, out of 5) as cumulative scores throughout all sections. The majority of the consensus was agreeable with the Discussion (Other) section of the checklist (median 4.8 (interquartile range (IQR) 4.8-4.8)), whereas the least agreed section was the Ground Truth (Expert(s) review) section (median 4.1 (IQR 3.9–4.2)) and the Methods (Outcomes) section (median 4.1 (IQR 4.1–4.1)) of the checklist. The final checklist after consensus and revision included a total of 8 sections and 50 questions.
Conclusions:
The HUMANE international consensus has reflected on further research on the potential of this checklist as an established consensus in improving the reliability and quality of research in this field.
DOI: https://doi.org/10.37349/edht.2024.00018
Aim:
Monitoring the prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) immunization in patients with autoimmune diseases is of particular concern to understand their response to the infection and to the vaccine. In fact, the immunological disorder and the immunosuppressive therapies could affect the serological response. SARS-CoV2 serological tests potentially provide this in
Methods:
From May to December 2020, a cohort of patients affected by primary biliary cholangitis (PBC), autoimmune hepatitis (AIH) and PBC/AIH overlap syndrome were screened with reverse transcription-polymerase chain reaction (RT-PCR) of nasopharyngeal swabs, rapid antigenic test and chemiluminescent serological test during routine follow-up.
Results:
The analysis of 42 patients was carried out: 18 (42.85%) PBC, 12 (28.57%) AIH and 12 (28.57%) PBC/AIH overlap syndromes. Only 2 patients (4.76%) resulted positive to the RNA, antigen and antibody detection tests, hence affected by SARS-CoV2 infection. 14 subjects out of 40 negative cases presented a positive serology
Conclusions:
Patients with liver autoimmune diseases present a high rate of false positive SARS-CoV2 serology. There
Aim:
Monitoring the prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) immunization in patients with autoimmune diseases is of particular concern to understand their response to the infection and to the vaccine. In fact, the immunological disorder and the immunosuppressive therapies could affect the serological response. SARS-CoV2 serological tests potentially provide this in
Methods:
From May to December 2020, a cohort of patients affected by primary biliary cholangitis (PBC), autoimmune hepatitis (AIH) and PBC/AIH overlap syndrome were screened with reverse transcription-polymerase chain reaction (RT-PCR) of nasopharyngeal swabs, rapid antigenic test and chemiluminescent serological test during routine follow-up.
Results:
The analysis of 42 patients was carried out: 18 (42.85%) PBC, 12 (28.57%) AIH and 12 (28.57%) PBC/AIH overlap syndromes. Only 2 patients (4.76%) resulted positive to the RNA, antigen and antibody detection tests, hence affected by SARS-CoV2 infection. 14 subjects out of 40 negative cases presented a positive serology
Conclusions:
Patients with liver autoimmune diseases present a high rate of false positive SARS-CoV2 serology. There
DOI: https://doi.org/10.37349/emed.2021.00055
This article belongs to the special issue Exploring Chronic Liver Disease
Aim:
Evaluation of real-world data regarding the use of omalizumab on lung function, asthma control, exacerbations, and oral corticosteroid (OCS).
Methods:
The single-centre, retrospective study included data from adult patients with severe allergic asthma treated with omalizumab
Results:
Data from 74 adults (mean age 51 years, 61% females, median IgE 276 kU/L), with severe allergic asthma, due to perennial allergens, who were treated
Conclusions:
The consistent improvement in lung function, asthma control, reduction in exacerbations, and OCS use throughout a minimum of five up to ten years confirms that omalizumab remains effective
Aim:
Evaluation of real-world data regarding the use of omalizumab on lung function, asthma control, exacerbations, and oral corticosteroid (OCS).
Methods:
The single-centre, retrospective study included data from adult patients with severe allergic asthma treated with omalizumab
Results:
Data from 74 adults (mean age 51 years, 61% females, median IgE 276 kU/L), with severe allergic asthma, due to perennial allergens, who were treated
Conclusions:
The consistent improvement in lung function, asthma control, reduction in exacerbations, and OCS use throughout a minimum of five up to ten years confirms that omalizumab remains effective
DOI: https://doi.org/10.37349/eaa.2024.00047
This article belongs to the special issue The Era of Biologics in Allergy
Aim:
As the primary response antibody with increasing use as a therapeutic immunoglobulin (Ig)
Methods:
Through a panel of 14 recombinant complementarity determining regions (CDRs)-grafted trastuzumab and pertuzumab VH1-7 IgMs subjected to bio-layer interferometry measurements, the interactions with the antigen human epidermal growth factor receptor 2 (Her2), Fc-mu receptor (FcμR), and superantigen Protein L (PpL) were investigated.
Results:
Significant effects from the V-regions to mitigate FcμR binding and the IgM C-region bidirectional effect modulating Her2 antigen engagements at the V-regions were found. Additional modulatory effects from superantigen PpL binding on the V-region of the kappa chain (Vκ) mitigating antigen binding were also found, revealing possible novel mechanisms of antibody superantigens that can be moderated by the antibody VH frameworks.
Conclusions:
These findings show that the oligomerisation of IgMs plays a significant role in FcμR, antigen, and superantigen binding that made IgM distinct from the other antibody isotypes and how these features should be considered during further development and protein engineering of IgM therapeutics.
Aim:
As the primary response antibody with increasing use as a therapeutic immunoglobulin (Ig)
Methods:
Through a panel of 14 recombinant complementarity determining regions (CDRs)-grafted trastuzumab and pertuzumab VH1-7 IgMs subjected to bio-layer interferometry measurements, the interactions with the antigen human epidermal growth factor receptor 2 (Her2), Fc-mu receptor (FcμR), and superantigen Protein L (PpL) were investigated.
Results:
Significant effects from the V-regions to mitigate FcμR binding and the IgM C-region bidirectional effect modulating Her2 antigen engagements at the V-regions were found. Additional modulatory effects from superantigen PpL binding on the V-region of the kappa chain (Vκ) mitigating antigen binding were also found, revealing possible novel mechanisms of antibody superantigens that can be moderated by the antibody VH frameworks.
Conclusions:
These findings show that the oligomerisation of IgMs plays a significant role in FcμR, antigen, and superantigen binding that made IgM distinct from the other antibody isotypes and how these features should be considered during further development and protein engineering of IgM therapeutics.
DOI: https://doi.org/10.37349/ei.2022.00083
Aim:
Racial disparities in opioid use disorder (OUD) management exist, however, and there is limited research on factors that influence opioid cessation in different population groups.
Methods:
We employed multiple machine learning prediction algorithms least absolute shrinkage and selection operator, random
Results:
Support vector machine models per
Conclusions:
These proof-of-concept findings provide avenues
Aim:
Racial disparities in opioid use disorder (OUD) management exist, however, and there is limited research on factors that influence opioid cessation in different population groups.
Methods:
We employed multiple machine learning prediction algorithms least absolute shrinkage and selection operator, random
Results:
Support vector machine models per
Conclusions:
These proof-of-concept findings provide avenues
DOI: https://doi.org/10.37349/emed.2020.00003
This article belongs to the special issue The Biological Basis of Substance Use Disorders
Mitochondria are present in all mammalian cells except matured red blood cells. Mitochondria consist of several metabolic pathways
Mitochondria are present in all mammalian cells except matured red blood cells. Mitochondria consist of several metabolic pathways
DOI: https://doi.org/10.37349/edd.2024.00039
This article belongs to the special issue Mitochondria and Lipid Signalling in Liver Diseases
Aim:
This study discloses the synthesis and the antimicrobial and anticancer activities of four molecules of structural basis saccharin-thiadiazolyl (4), saccharin-pyridyl (6, 8), and tetrazole-thiadiazolyl (11).
Methods:
Antimicrobial properties of the molecules were evaluated by the well-diffusion method, against Gram-positive bacteria [Staphylococcus aureus American Type Culture Collection (ATCC) 25923, Staphylococcus epidermidis ATCC 12228, Mycobacterium smegmatis ATCC 607], Gram-negative bacteria (Pseudomonas aeruginosa ATCC 27853) and yeast (Saccharomyces cerevisiae ATCC 2601 and Candida albicans ATCC 10231) strains. The anticancer activity of the compounds was assessed through i) proliferation assays
Results:
Compound 11 exhibited significant inhibitory activity against S. aureus and S. epidermidis, with the further molecules demonstrating some inhibitory potential against all the tested Gram-positive, Gram-negative, and yeast strains. Similarly, derivative 11 showed an interesting antiproliferative activity against human colon adenocarcinoma (HCT116), human breast adenocarcinoma (MCF-7), and melanoma (A375) cells, with 50% growth inhibition (GI50) values varying from 3.55 µmol/L to 11.5 µmol/L, in the same order of magnitude of those shown by etoposide. Treatment of brain-like glioblastoma cells (U87) with 11, at the concentration of 100 µg/mL, induced a decrease in cell viability by 50% after 48 h and 72 h. Besides, results attained
Conclusions:
This investigation reveals the potential of distinct azole-based conjugates, in particular the tetrazole-thiadiazolyl (11) derivative, as scaffolds worth further investigations, in the frame of antimicrobial and antineoplastic chemotherapy.
Aim:
This study discloses the synthesis and the antimicrobial and anticancer activities of four molecules of structural basis saccharin-thiadiazolyl (4), saccharin-pyridyl (6, 8), and tetrazole-thiadiazolyl (11).
Methods:
Antimicrobial properties of the molecules were evaluated by the well-diffusion method, against Gram-positive bacteria [Staphylococcus aureus American Type Culture Collection (ATCC) 25923, Staphylococcus epidermidis ATCC 12228, Mycobacterium smegmatis ATCC 607], Gram-negative bacteria (Pseudomonas aeruginosa ATCC 27853) and yeast (Saccharomyces cerevisiae ATCC 2601 and Candida albicans ATCC 10231) strains. The anticancer activity of the compounds was assessed through i) proliferation assays
Results:
Compound 11 exhibited significant inhibitory activity against S. aureus and S. epidermidis, with the further molecules demonstrating some inhibitory potential against all the tested Gram-positive, Gram-negative, and yeast strains. Similarly, derivative 11 showed an interesting antiproliferative activity against human colon adenocarcinoma (HCT116), human breast adenocarcinoma (MCF-7), and melanoma (A375) cells, with 50% growth inhibition (GI50) values varying from 3.55 µmol/L to 11.5 µmol/L, in the same order of magnitude of those shown by etoposide. Treatment of brain-like glioblastoma cells (U87) with 11, at the concentration of 100 µg/mL, induced a decrease in cell viability by 50% after 48 h and 72 h. Besides, results attained
Conclusions:
This investigation reveals the potential of distinct azole-based conjugates, in particular the tetrazole-thiadiazolyl (11) derivative, as scaffolds worth further investigations, in the frame of antimicrobial and antineoplastic chemotherapy.
DOI: https://doi.org/10.37349/eds.2023.00028
Aim:
Physical activity (PA) is increasingly used as a patient-centered means to treat and/or cope with pain and other symptomology resulting from clinical health conditions. Despite the increasing use of wearable sensors to track PA in healthy and patient cohorts, few algorithms are equally accurate in assessing sedentary and light PA as moderate and vigorous. Given that many older adults and patient cohorts are less active, there is a need
Methods:
Triaxial accelerometers were worn on the wrist and hip during 14 standardized laboratory-based activities in 37 healthy adults across the lifespan [19–65 years, 19 females (F)]. Combined with previously reported energy expenditure data, linear and power equations trans
Results:
The nonlinear algorithm provided equally accurate measures of PA energy expenditure as linear approaches, with the added advantage of being able to estimate even low energy expenditure, a necessary outcome to differentiate sedentary and light PA. Further, the nonlinear algorithm produced a slightly better estimate of PA when using wrist than hip accelerometry.
Conclusions:
A simple nonlinear algorithm provides a better means
Aim:
Physical activity (PA) is increasingly used as a patient-centered means to treat and/or cope with pain and other symptomology resulting from clinical health conditions. Despite the increasing use of wearable sensors to track PA in healthy and patient cohorts, few algorithms are equally accurate in assessing sedentary and light PA as moderate and vigorous. Given that many older adults and patient cohorts are less active, there is a need
Methods:
Triaxial accelerometers were worn on the wrist and hip during 14 standardized laboratory-based activities in 37 healthy adults across the lifespan [19–65 years, 19 females (F)]. Combined with previously reported energy expenditure data, linear and power equations trans
Results:
The nonlinear algorithm provided equally accurate measures of PA energy expenditure as linear approaches, with the added advantage of being able to estimate even low energy expenditure, a necessary outcome to differentiate sedentary and light PA. Further, the nonlinear algorithm produced a slightly better estimate of PA when using wrist than hip accelerometry.
Conclusions:
A simple nonlinear algorithm provides a better means
DOI: https://doi.org/10.37349/emd.2024.00066
Aim:
Recent randomized controlled trials (RCTs) have tested the efficacy of glucagon-like peptide-1 receptor agonists (GLP-1 RA) to specifically treat non-alcoholic fatty liver disease (NAFLD). We per
Methods:
We systematically searched PubMed and ClinicalTrials.Gov databases utilizing specific terms to identify placebo-controlled or head-to-head RCTs (last research on March 1, 2020) involving NAFLD patients with the aim of evaluating the efficacy of GLP-1 RAs to treat NAFLD/NASH. Primary outcomes were changes in serum liver enzymes, liver fat content, or histologic resolution of NASH. Weighted mean differences (WMD) were used to test the differences between the treatment arms.
Results:
Overall, we found 7 placebo-controlled or head-to-head RCTs involving 472 middle-aged individuals (66% men; 77% with established diabetes) followed
Conclusions:
GLP-1 RAs (mostly liraglutide) seem to be a promising treatment option
Aim:
Recent randomized controlled trials (RCTs) have tested the efficacy of glucagon-like peptide-1 receptor agonists (GLP-1 RA) to specifically treat non-alcoholic fatty liver disease (NAFLD). We per
Methods:
We systematically searched PubMed and ClinicalTrials.Gov databases utilizing specific terms to identify placebo-controlled or head-to-head RCTs (last research on March 1, 2020) involving NAFLD patients with the aim of evaluating the efficacy of GLP-1 RAs to treat NAFLD/NASH. Primary outcomes were changes in serum liver enzymes, liver fat content, or histologic resolution of NASH. Weighted mean differences (WMD) were used to test the differences between the treatment arms.
Results:
Overall, we found 7 placebo-controlled or head-to-head RCTs involving 472 middle-aged individuals (66% men; 77% with established diabetes) followed
Conclusions:
GLP-1 RAs (mostly liraglutide) seem to be a promising treatment option
DOI: https://doi.org/10.37349/emed.2020.00008
This article belongs to the special issue Exploring Type 2 Diabetes Mellitus
Aim:
The present study aims to generate chimeric mouse single-chain variable fragment (scFv) and immunoglobulin G1 (IgG1) crystallizable fragment (Fc) antibody against disialoganglioside (GD2)
Methods:
Vector
Results:
Using plasmid fusion-human IgG1-Fc2 tag vector (pFUSE-hIgG1-Fc2), a plasmid vector encoding chimeric mouse scFv and hIgG1
Conclusions:
The results indicate that chimeric scFv-hIgG
Aim:
The present study aims to generate chimeric mouse single-chain variable fragment (scFv) and immunoglobulin G1 (IgG1) crystallizable fragment (Fc) antibody against disialoganglioside (GD2)
Methods:
Vector
Results:
Using plasmid fusion-human IgG1-Fc2 tag vector (pFUSE-hIgG1-Fc2), a plasmid vector encoding chimeric mouse scFv and hIgG1
Conclusions:
The results indicate that chimeric scFv-hIgG
DOI: https://doi.org/10.37349/etat.2023.00188
This article belongs to the special issue Novel Strategies and Targets for Immunotherapy of Cancer
Healthcare strives to ensure overall physical, mental, and emotional well-being
Healthcare strives to ensure overall physical, mental, and emotional well-being
DOI: https://doi.org/10.37349/edht.2024.00021
This article belongs to the special issue Data-informed Decision Making in Healthcare
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a multifactorial inflammatory disease of the mucous membranes of the nose and paranasal sinuses. Eosinophilic inflammation is described as a common endotype. The anti-interleukin-5 (IL-5) antibody mepolizumab was approved in November 2021 as an add-on therapy to intranasal glucocorticosteroids
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a multifactorial inflammatory disease of the mucous membranes of the nose and paranasal sinuses. Eosinophilic inflammation is described as a common endotype. The anti-interleukin-5 (IL-5) antibody mepolizumab was approved in November 2021 as an add-on therapy to intranasal glucocorticosteroids
DOI: https://doi.org/10.37349/eaa.2024.00027
This article belongs to the special issue Update on Chronic RhinoSinusitis
Aim:
Rice, one of the most widely consumed staple foods globally, relies on amylose content
Methods:
Certified rice flour standards of varying amylose levels have been analyzed using a quantum-cascade LPAS system. Preliminary analysis utilized Fourier trans
Results:
LPAS spectra, recorded between 7.0–11.0 μm, displayed two broad bands, showing a linear increase in signal with amylose content, especially notable in the specific fingerprint region within 8.5–10.0 μm. The prominent peak at 9.3 μm exhibited a high linear correlation with amylose levels (R2 > 0.99). PCA effectively differentiated rice flour samples, while PLS accurately predicted amylose content. The difference between predicted and actual amylose is significantly less than the statistical error of the measurement.
Conclusions:
LPAS combined with chemometric analysis emerges as a promising non-destructive method
Aim:
Rice, one of the most widely consumed staple foods globally, relies on amylose content
Methods:
Certified rice flour standards of varying amylose levels have been analyzed using a quantum-cascade LPAS system. Preliminary analysis utilized Fourier trans
Results:
LPAS spectra, recorded between 7.0–11.0 μm, displayed two broad bands, showing a linear increase in signal with amylose content, especially notable in the specific fingerprint region within 8.5–10.0 μm. The prominent peak at 9.3 μm exhibited a high linear correlation with amylose levels (R2 > 0.99). PCA effectively differentiated rice flour samples, while PLS accurately predicted amylose content. The difference between predicted and actual amylose is significantly less than the statistical error of the measurement.
Conclusions:
LPAS combined with chemometric analysis emerges as a promising non-destructive method
DOI: https://doi.org/10.37349/eff.2024.00050
This article belongs to the special issue Metrological Aspects in the Analysis of Nutrients, Functional Compounds, Additives and Contaminants in Food and Feed
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by both non-motor and motor symptoms, due to the loss of dopamine-producing neurons in the brain. Monoamine oxidase-B (MAO-B) inhibitors are essential in the treatment of PD, as they increase dopamine levels and could potentially slow down the progression of the disease. MAO-B inhibitors block the ability of the enzyme to degrade dopamine in the brain. MAO-B inhibitors work by inhibiting this enzyme, which raises dopamine levels and helps reduce motor symptoms, such as akinesia and stiffness in the muscles. In addition to their impact on dopamine levels, MAO-B inhibitors may possess neuroprotective properties. Research indicates that these inhibitors can shield neurons from the harmful byproducts of dopamine breakdown, such as dihydroxy acetaldehyde and hydrogen peroxide. This neuroprotective effect could potentially slow the progression of PD and protect against neuronal damage. MAO-B inhibitors are effective in treating both advanced and early stages of PD. They are recommended as initial treatments
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by both non-motor and motor symptoms, due to the loss of dopamine-producing neurons in the brain. Monoamine oxidase-B (MAO-B) inhibitors are essential in the treatment of PD, as they increase dopamine levels and could potentially slow down the progression of the disease. MAO-B inhibitors block the ability of the enzyme to degrade dopamine in the brain. MAO-B inhibitors work by inhibiting this enzyme, which raises dopamine levels and helps reduce motor symptoms, such as akinesia and stiffness in the muscles. In addition to their impact on dopamine levels, MAO-B inhibitors may possess neuroprotective properties. Research indicates that these inhibitors can shield neurons from the harmful byproducts of dopamine breakdown, such as dihydroxy acetaldehyde and hydrogen peroxide. This neuroprotective effect could potentially slow the progression of PD and protect against neuronal damage. MAO-B inhibitors are effective in treating both advanced and early stages of PD. They are recommended as initial treatments
DOI: https://doi.org/10.37349/ent.2024.00085
Aim:
The pleiotropic effect of fibroblast growth factor 2 (FGF2) on promoting myogenesis, angiogenesis, and innervation makes it an ideal growth factor
Methods:
Heparin was passively adsorbed or carbodiimide-conjugated to microthreads, creating a biomimetic binding strategy, mimicking FGF2 sequestration in the extracellular matrix (ECM). It was also evaluated whether FGF2 incorporated into fibrin microthreads would yield sustained release. It was hypothesized that heparin-conjugated and co-incorporated (co-inc) fibrin microthreads would facilitate sustained release of FGF2 from the scaffold and enhance in vitro myoblast proliferation and outgrowth.
Results:
Toluidine blue staining and Fourier trans
Conclusions:
It was anticipated that the combined effects of fibrin microthread structural properties, topographic alignment cues, and FGF2 release profiles will facilitate the fabrication of a biomimetic scaffold that enhances the regeneration of functional muscle tissue
Aim:
The pleiotropic effect of fibroblast growth factor 2 (FGF2) on promoting myogenesis, angiogenesis, and innervation makes it an ideal growth factor
Methods:
Heparin was passively adsorbed or carbodiimide-conjugated to microthreads, creating a biomimetic binding strategy, mimicking FGF2 sequestration in the extracellular matrix (ECM). It was also evaluated whether FGF2 incorporated into fibrin microthreads would yield sustained release. It was hypothesized that heparin-conjugated and co-incorporated (co-inc) fibrin microthreads would facilitate sustained release of FGF2 from the scaffold and enhance in vitro myoblast proliferation and outgrowth.
Results:
Toluidine blue staining and Fourier trans
Conclusions:
It was anticipated that the combined effects of fibrin microthread structural properties, topographic alignment cues, and FGF2 release profiles will facilitate the fabrication of a biomimetic scaffold that enhances the regeneration of functional muscle tissue
DOI: https://doi.org/10.37349/ebmx.2024.00006
Aim:
Fragment crystallizable (Fc) glycans modulate
Methods:
Molecular dynamics simulations were used to generate con
Results:
Two important interaction patterns have been observed. The first is the strong but non-specific interactions between two carbohydrate chains in free antibody. Secondly, it has been found that N-glycans carbohydrate chains can directly interact with CH3 domain in free antibody, and that the distance distribution between carbohydrate chains and CH3 domain clearly differentiate the free antibody, antibody-antigen complex, antibody-hFcγRI complex, and final antibody-antigen-hFcγRI complex.
Conclusions:
N-glycans partially acts as allosteric sensor and respond to antigen and hFcγRI binding.
Aim:
Fragment crystallizable (Fc) glycans modulate
Methods:
Molecular dynamics simulations were used to generate con
Results:
Two important interaction patterns have been observed. The first is the strong but non-specific interactions between two carbohydrate chains in free antibody. Secondly, it has been found that N-glycans carbohydrate chains can directly interact with CH3 domain in free antibody, and that the distance distribution between carbohydrate chains and CH3 domain clearly differentiate the free antibody, antibody-antigen complex, antibody-hFcγRI complex, and final antibody-antigen-hFcγRI complex.
Conclusions:
N-glycans partially acts as allosteric sensor and respond to antigen and hFcγRI binding.
DOI: https://doi.org/10.37349/ei.2021.00004
Aim:
Respiratory failure is common after esophagectomy
Methods:
A single-center observational study from China was conducted on 262 patients with EC who underwent thoracoscopic esophagectomy between April 2014 and June 2016. The patients were divided into two groups: group I (respiratory failure) and group II (without respiratory failure). Demographic and perioperative variables, tumor-related factors, surgical factors, Acute Physiology and Chronic Health Evaluation II (APACHE II) scores, and clinical course were compared between the groups. Univariable and multivariable logistic regression analyses were per
Results:
Among the 262 patients, 24 (9.2%) developed respiratory failure. Univariable analysis revealed several risk factors, including age, smoking, comorbidities, partial pressure of oxygen (PO2), partial pressure of carbon dioxide (PCO2),
Conclusions:
As a common complication following esophagectomy
Aim:
Respiratory failure is common after esophagectomy
Methods:
A single-center observational study from China was conducted on 262 patients with EC who underwent thoracoscopic esophagectomy between April 2014 and June 2016. The patients were divided into two groups: group I (respiratory failure) and group II (without respiratory failure). Demographic and perioperative variables, tumor-related factors, surgical factors, Acute Physiology and Chronic Health Evaluation II (APACHE II) scores, and clinical course were compared between the groups. Univariable and multivariable logistic regression analyses were per
Results:
Among the 262 patients, 24 (9.2%) developed respiratory failure. Univariable analysis revealed several risk factors, including age, smoking, comorbidities, partial pressure of oxygen (PO2), partial pressure of carbon dioxide (PCO2),
Conclusions:
As a common complication following esophagectomy
DOI: https://doi.org/10.37349/emed.2023.00195
Desensitization (DSZ) or oral tolerance induction is increasingly used in children who do not outgrow their food allergies. Off-label omalizumab (OMZ) is used as adjuvant therapy
Desensitization (DSZ) or oral tolerance induction is increasingly used in children who do not outgrow their food allergies. Off-label omalizumab (OMZ) is used as adjuvant therapy
DOI: https://doi.org/10.37349/eaa.2024.00025
This article belongs to the special issue The Different Faces of Food Allergy
