Summary of validation parameters for HPLC method

Validation parametersOXYPMB
Absorption maxima (λ max; nm)303
Linearity and range (μg/mL)5–25
Coefficient of determination (R2)0.99150.9961
Regression equation (y)3,280.7x – 9,906.62,437.6x – 10,926
Slope (m)3,280.72,437.6
Intercept (c)9,90610,926
LOD (μg/mL)0.1970.173
LOQ (μg/mL)0.5990.525
Precision1.210.89
Robustness1.5690.879