Table Info

Table 1.

Optimization of chromatographic conditions for reversed-phase (RP)-HPLC method

Chromatographic parameters	Variations	Peak shape
Mobile phase composition (v/v)
ACN:Methanol:Water	50:30:20	No ideal peak
ACN:Water	50:50	Broad peak
	60:40	Not symmetric
	70:30	Sharp resolved peak, tailing
	80:20	Well resolved peak
pH variation
ACN:Orthophosphoric acid (pH 4.0);	4.0	Broad peaks
optimized ratio (80:20)	3.0	Resolved sharp peak, tailing
optimized ratio (80:20)	3.5	Sharp, well separated peaks

Supplementary materials

The supplementary material for this article is available at: https://www.explorationpub.com/uploads/Article/file/10082_sup_1.pdf.

Declarations

Author contributions

DS: Conceptualization, Investigation, Writing—original draft. TC: Conceptualization, Investigation, Writing—original draft. BDK: Validation, Writing—review & editing, Supervision.

Conflicts of interest

The authors declared that they have no conflict of interest.

Ethical approval

Not applicable.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

Not applicable.

Funding

Not applicable.

Copyright

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