Exploration of Endocrine and Metabolic Diseases

Table 1. Target, host, and source of primary and secondary antibodies employed for immunofluorescent imaging experiments.

Antibody	Target/Host	Dilution	Source/Fluorescent dilution and source^*
Primary antibodies	Insulin/Mouse	1:500	Abcam, ab6995
	Glucagon/Guinea pig	1:200	Raised in-house PCA2/4
	PYY/Rabbit	1:500	Abcam, ab22663
	SST/Rat	1:500	Biorad, 8330-009
	GLP-1/Rabbit	1:200	Raised in-house XJIC8
	GIP/Rabbit	1:400	RIC34/111J, kindly donated by Professor L Morgan, Guildford, UK
	Ki-67/Rabbit	1:200	Abcam, ab15580
Secondary antibodies	Mouse/Goat IgG	1:500	A-11005, Alexa Flour 594, Invitrogen, UK
	Guinea pig/Goat IgG	1:500	A-11073, Alexa Flour 488, Invitrogen, UK
	Rabbit/Goat IgG	1:500	A-11012, Alexa Flour 594, Invitrogen, UK
	Rat/Goat IgG	1:500	A-11006, Alexa Flour 488, Invitrogen, UK

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^*: for secondary antibodies. GLP-1: glucagon-like peptide-1; PYY: peptide YY; GLP-1: glucagon-like peptide-1; GIP: glucose-dependent insulinotropic polypeptide; SST: somatostatin.

Supplementary materials

The supplementary figure for this article is available at: https://www.explorationpub.com/uploads/Article/file/101475_sup_1.pdf.

Declarations

Author contributions

AS: Conceptualization, Data curation, Formal analysis, Writing—original draft, Writing—review & editing, Investigation. VNI: Data curation, Formal analysis, Writing—original draft. OM: Data curation, Formal analysis. PRF: Conceptualization, Supervision, Visualization, Writing—review & editing. CRM: Conceptualization, Funding acquisition, Supervision, Visualization, Writing—review & editing. DK: Conceptualization, Data curation, Supervision, Visualization, Writing—review & editing. All authors read and approved the submitted version.

Conflicts of interest

Dr. Dawood Khan, who is the Guest Editor of Exploration of Endocrine and Metabolic Diseases, had no involvement in the decision-making or the review process of this manuscript. The other authors declare no conflicts of interest.

Ethical approval

All experiments were conducted under the UK Animals (Scientific Procedures) Act 1986 & EU Directive 2010/63EU as well as the UK Home Office animal project license number PPL2902, and approved by the University of Ulster Animal Welfare and Ethical Review Body (AWERB).

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

The datasets supporting the findings of this study are available from the corresponding author upon reasonable request.

Funding

These studies were supported by Diabetes UK RD Lawrence Fellowship grant to RCM and Ulster University strategic funding. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

Publisher’s note

Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.

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