Exploration of Endocrine and Metabolic Diseases

Table 1. Primer sequences.

Species	Gene	Primer sequence (5’–3’)	Accession number
Mouse	Actb (β-actin)	F: GCAAGTGCTTCTAGGCGGAC	NM_007393.5
Mouse	Actb (β-actin)	R: AAGAAAGGGTGTAAAACGCAGC	NM_007393.5
Mouse	Grk2 (GRK2)	F: GCGCCAGCAAGAAGATCCT	NM_001289480.1
Mouse	Grk2 (GRK2)	R: GCAGAAGTCCCGGAAAAGCA	NM_001289480.1
Mouse	Ucp1 (UCP-1)	F: AGGCTTCCAGTACCATTAGGT	NM_009463.3
Mouse	Ucp1 (UCP-1)	R: CTGAGTGAGGCAAAGCTGATTT	NM_009463.3
Mouse	Nrf1 (NRF1)	F: TCTGCTGTGGCTGATGGAGAGG	NM_001164230.2
Mouse	Nrf1 (NRF1)	R: GATGCTTGCGTCGTCTGGATGG	NM_001164230.2
Mouse	Cox4i1 (COX-IV)	F: GTACCGCATCCAGTTTAACGA	NM_053094.2
Mouse	Cox4i1 (COX-IV)	R: CCATACACATAGCTCTTCTCCCA	NM_053094.2
Mouse	Ppara (PPARα)	F: AGAGCCCCATCTGTCCTCTC	NM_001113418.2
Mouse	Ppara (PPARα)	R: ACTGGTAGTCTGCAAAACCAAA	NM_001113418.2
Mouse	Cidea (CIDEA)	F: TGCTCTTCTGTATCGCCCAGT	NM_001285731.1
Mouse	Cidea (CIDEA)	R: GCCGTGTTAAGGAATCTGCTG	NM_001285731.1
Mouse	Prdm16 (PRDM16)	F: CCACCAGCGAGGACTTCAC	NM_001177332.2
Mouse	Prdm16 (PRDM16)	R: GGAGGACTCTCGTAGCTCGAA	NM_001177332.2
Mouse	Ebf2 (EBF2)	F: GCGGTTCCAGGTCGTGTTGTC	NM_001113197.2
Mouse	Ebf2 (EBF2)	R: GCCTTCTTGCTCTCCTTCCATGC	NM_001113197.2
Mouse	Tbx1 (TBX1)	F: CACCAAGGCAGGCAGACGAATG	NM_011537.3
Mouse	Tbx1 (TBX1)	R: CATCTACGGGCACAAAGTCCATGAG	NM_011537.3
Mouse	Ppargc1a (PGC-1α)	F: TATGGAGTGACATAGAGTGTGCT	NM_008904.2
Mouse	Ppargc1a (PGC-1α)	R: CCACTTCAATCCACCCAGAAAG	NM_008904.2

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GRK2: G protein-coupled receptor kinase 2; UCP-1: uncoupling protein-1; NRF1: nuclear respiratory factor 1; COX-IV: cytochrome c oxidase IV; PPARα: peroxisome proliferators-activated receptor α; CIDEA: cell death inducing DFFA like effector A; PRDM16: PR domain-containing 16; EBF2: early B cell factor 2; PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1 α.

Supplementary materials

The supplementary figures for this article are available at: https://www.explorationpub.com/uploads/Article/file/101441_sup_1.pdf.

Declarations

Author contributions

Zhuqi W: Investigation, Formal analysis, Visualization, Writing—original draft. JL: Investigation, Validation. Ziqing W and HZ: Investigation. XS: Conceptualization, Supervision, Writing—review & editing. KL: Conceptualization, Supervision, Visualization, Writing—review & editing. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The animal protocol was approved by the Ethics Committee of China Pharmaceutical University (registration code: 2022-08-006).

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

Datasets are available on request.

Funding

The work was supported by the National Natural Science Foundation of China (Grant No. [82174009]). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

Publisher’s note

Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.

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