Analysis of the serum anti-UBE2E3 antibody (UBE2E3-Ab) levels among HDs and patients with diabetes mellitus (DM)
Sample information and alpha analysis
(A) Sample information
HD
DM
Total sample number
81
275
Male/Female
46/35
156/119
Age (Average ± SD)
45.2 ± 11.0
63.1 ± 12.0
(B) Alpha analysis (antibody level)
UBE2E3-Ab
HD
Average
4,003
SD
2,709
Cutoff value
9,421
Positive No.
4
Positive rate (%)
4.9%
DM
Average
7,034
SD
6,207
Positive No.
61
Positive rate (%)
22.2%
P (vs. HD)
9.8E-10
The upper panel (A) represents the total numbers, sex (male and female), and ages (average ± SD). The lower panel (B) provides a summary of the serum antibody levels, measured as Alpha photon counts using amplified luminescence proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) with UBE2E3-GST protein. Cutoff values were defined as the average healthy donor (HD) values plus two SD, and positive samples higher than the cutoff value were scored. P value < 0.05 and positive rate > 10% are marked in bold text. A scatter dot plot of the same results is shown in Figure 1B
The authors would like to thank Prof. Masaki Takiguchi (Chiba University), Prof. Kenichiro Kitamura (Yamanishi University), Prof. Hao Wang (Jinan University), Dr. Xiao-Meng Zhang (Chiba University), Dr. Mari Oba (National Center of Neurology and Psychiatry, Tokyo), Dr. Risa Kimura (Chiba University), for supporting this research as well as Ms. Seiko Otsuka, Masae Suzuki, Chiho Kusaka, Satoko Ishibashi, and Akiko Kimura for technical assistance.
The authors declare that they have no conflicts of interest.
Ethical approval
This study was approved by the Local Ethical Review Board of Chiba University, Graduate School of Medicine [approved numbers: 2012-438, 2014-44, 2016-86, 2017-251, 2018-320, 2020-1129, 2022-623, 2023-836], Toho University, Faculty of Medicine [approved numbers: A18103_A17052_A16035_A16001_26095_25024_ 24038_22047, 25131_23005], Toho University Omori Medical Center [approved number: 26-255], and Port Square Kashiwado Clinic [approved number: 2012‑001] as well as by the review boards of the participating hospitals. All experimental procedures were performed in accordance with the Declaration of Helsinki, version 2013.
Consent to participate
Written informed consent was obtained from all participants by following the protocols approved by their institutional ethical committees.
This work was supported, in part, by research grants from the Japan Science and Technology Agency [JST: Exploratory Research No. 14657335], Japan Science and Technology Agency SPRING [grant nos. JPMJSP2109], and research grants from Japan Society for the Promotion of Science KAKENHI [Grant Number: 20K17953, 22K07273, 20K07810, 21K19437, 21K08695, 16K10520]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.
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