From:  Biotyping and antibiotic susceptibility of animal streptococci from raw/thermized milks of native Epirus sheep breeds

 API 20 STREP-based identification and biotyping of the Streptococcus isolates from raw or thermized sheep milk (compared to the genomic identification in Table 1)1.

TestReaction/EnzymesStreptococcus parauberisStreptococcus equinusStreptococcus gallolyticusStreptococcus lutetiensis
BiotypeI (4)II (3)(6)(1)(2)
VPAcetoin production++++
HIPHydrolysis (hipuric acid)++
ESCβ-glucosidase hydrolysis++
PYRAPyrolidonyl arylamidase
αGALα-galactosidase+++
βGURβ-glucuronidase+
βGALβ-galactosidase++
PALAlkaline phosphatase+
LAPLeucine aminopeptidase+++++
ADHArginine dihydrolase++
RIBD-ribose (acidification)++
ARAL-arabinose (acidification)
MAND-mannitol (acidification)
SORD-sorbitol (acidification)+/(+)
LACD-lactose (acidification)+++++
TRED-trehalose (acidification)+++++
INUInulin (acidification)+–/(+)**
RAFD-raffinose (acidification)+++
AMDStarch (acidification)–/+*++
GLYGGlycogen (acidification)++
βHEMβ-hemolysis
API code30434103063630
3063631*
0250453
0250473**
56504505240453
API-IDS. agalactiae AcceptableS. uberis UncertainS. pneumoniae
Good/
Very good
S. gallolyticus
pasteurianus
Excellent
S. equinus
(S. bovis I/II)
Very good

1 The number of isolates (in brackets) corresponds with the isolate codes as grouped for each species in Table 1. * Isolate KFM 35 of S. parauberis-biotype II was starch-positive; ** Isolates KTM 2, KTM 8 and KTM 9 of S. equinus gave a weak positive acidification reaction with inulin.