Exploration of Foods and Foodomics

Table 4. Cytotoxicity as LDH activity and intracellular ROS scavenging in Caco2-TC7 cells treated with bioaccessible fractions (DF_b, DI_b, DB_b and RB_b).

Sample	Dilution	SP (mg/mL)	LDH (% cytotoxicity)	% ROS inhibition
DF_b	-	20	19 ± 1^β	92 ± 1^δ
	1/5	4	16 ± 2^αβ	87 ± 2^γδ
	1/10	2	16 ± 5^αβ	80 ± 4^βγ
	1/20	1	14 ± 1^α	58 ± 5^α
DI_b	-	8.3	9 ± 1^α	92 ± 2^δ
	1/5	1.66	8 ± 2^α	86 ± 1^γ
	1/10	0.83	8 ± 2^α	74 ± 3^β
	1/20	0.42	7 ± 4^α	59 ± 4^α
DB_b	-	2	27.9 ± 0.1^δ	76 ± 2^δ
	1/5	0.4	1.0 ± 0.1^α	64 ± 1^γ
	1/10	0.2	6 ± 1^β	38 ± 2^β
	1/20	0.1	14 ± 3^γ	19 ± 6^α
RB_b	-	0.4	62 ± 1^δ	-
	1/5	0.08	43 ± 1^γ	-
	1/10	0.04	31 ± 4^β	-
	1/20	0.02	16 ± 2^α	-

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SP: soluble protein. Means followed by different superscript letters indicate significant differences between the dilutions of each sample (P < 0.05). Repeated superscript letters indicate that the means were not significantly different (P > 0.05).

Declarations

Acknowledgements

The authors thank Bioseminis (https://bioseminis.com/) for providing yellow pea seeds and Dr. Fabricio Lareu (technical staff) for their assistance in handling cell cultures.

The authors M.A.C., S.G.F., and V.A.T. are members of the INNOPROT network (Network of Innovation in the processing of plant proteins endemic to Latin America), funded by CYTED (ref. 124RT0164).

Author contributions

MAC: Investigation, Methodology, Formal analysis, Visualization, Writing—original draft, Writing—review & editing. SFGF: Methodology, Formal analysis. AF: Methodology, Formal analysis, Writing—review & editing. VAT: Conceptualization, Methodology, Supervision, Writing—original draft, Writing—review & editing. All authors read and approved the submitted version.

Conflicts of interest

There are no conflicts to declare.

Ethical approval

Not applicable.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.

Funding

Support for this research came from grants X870 from Universidad Nacional de La Plata (UNLP) and PICT-2020-1367 from Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

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Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.

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