Fourier-transform infrared spectroscopy (FT-IR) spectroscopic analysis of the crude methanolic extract of B. asiatica and correlation with identified bioactive compounds.
Wave number (cm–1)
Functional group/vibration
Tentative assignment to compounds identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS)
3,983.78
O-H stretching
Hydroxyl groups in phenolics and anthocyanins (cyanidin-3-O-glucoside, pelargonidin-3-O-glucoside)
2,924.55–2,852.79
C-H stretching (aliphatic)
Aliphatic chains in catechin, epicatechin, and fatty acid derivatives
1,721.07
C=O stretching
Carbonyl groups in glycosidic linkages, esters, or ketones of glycosylated flavonoids and anthocyanins
1,600.11–1,514.08
Aromatic C=C stretching
Conjugated aromatic rings in flavonoids and berberine-type alkaloids
1,384.26
O-H bending/deformation
Hydroxyl groups in phenolic compounds and anthocyanins
1,266.85–1,071.53
C-O stretching
Glycosidic bonds in glycosylated flavonoids and sugars
873.75–779.33
Aromatic C-H out-of-plane bending
Substituted aromatic rings in isoquinoline alkaloids and complex phenolics
619.69
C-C skeleton vibration
Aromatic ring backbone, supporting polyphenolic structures
Authors acknowledge the support and cooperation to Analytical and Instrumentation Facility, NCS Group, Nagpur, Maharashtra for LC-MS/MS and FT-IR analysis.
Author contributions
MK and TK: Conceptualization, Formal analysis, Writing—original draft. NV and PB: Formal analysis, Methodology, Writing—review & editing. Rajesh K: Formal analysis, Methodology, Writing—review & editing, Formal analysis, Methodology. MH and Ravendra K: Project administration, Supervision, Writing—review & editing. EdAFJ: Formal analysis, Methodology. MSdO: Writing—review & editing. All authors read and approved the submitted version.
Conflicts of interest
The authors declare that they have no conflicts of interest.
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