Mean ± standard deviation (n = 3). Where C: control diet, T1: diet with 1 mL/kg inclusion level of the essential oil nanoemulsion, T2: diet with 3 mL/kg inclusion level of the essential oil nanoemulsion, T3: diet with 5 mL/kg inclusion level of the essential oil nanoemulsion.
Declarations
Acknowledgments
We gratefully acknowledge Mr. Ioannis Agrokostas (Roi Pelle Snail Farm, Magnesia, Greece) for supplying the snails and feed used in the experiments and the undergraduate students Apostolia Tsitsaki and Ioannis Tzoutzoulis for their assistance during the experimental procedure.
The authors declare that they have no conflicts of interest.
Ethical approval
The authors confirm that the ethical policies of the journal, as noted on the journal’s author guidelines page, have been adhered to, and the appropriate ethical review committee approval has been received. The authors confirm that they have followed EU standards for the protection of animals used for scientific purposes and feed legislation. No ethical approval was required for using Cornu aspersum maximum in animal studies. However, our experiments were conducted according to the internal guidelines of the University of Thessaly, Greece.
Consent to participate
Not applicable.
Consent to publication
Not applicable.
Availability of data and materials
The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.
Funding
This study was part of the project coded ΔΜΡ1-0010874 financed under the Partnership and Cooperation Agreement (NSRF) 2014–2020, the European Regional Development Fund (ERDF), and National Resources, Operational Program entitled “West Macedonia, Action 2014–2020”. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.
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