Recovery efficiency of Ascaris suum eggs in artificially contaminated HEP 1 (leafy vegetables and orange juice) across four processing protocols comparing homogenization methods and dissociation solutions.
| Nº protocol | Nº of eggs recovered trial 1 (%) | Nº of eggs recovered trial 2 (%) | Nº of eggs recovered trial 3 (%) | Final average of recovered eggs (%) | Standard deviation | p1 | p2 |
|---|---|---|---|---|---|---|---|
| Dose 1—207 eggs | |||||||
| 1 | 157 (76) | 129 (62) | 117 (57) | 134 (65) | 20 | 0.01 | 0.110 |
| 2 | 134 (65) | 164 (79) | 112 (54) | 137 (66) | 26 | ||
| 3 | 109 (53) | 106 (51) | 104 (50) | 106 (51) | 2.5 | ||
| 4 | 148 (71) | 117 (57) | 109 (53) | 125 (60) | 21 | ||
| Dose 2—76 eggs | |||||||
| 1 | 38 (50) | 48 (63) | 32 (42) | 39 (52) | 8 | 0.74 | 0.204 |
| 2 | 39 (51) | 42 (55) | 45 (59) | 42 (55) | 3 | ||
| 3 | 39 (51) | 44 (58) | 41 (54) | 41 (54) | 2.5 | ||
| 4 | 38 (50) | 36 (47) | 34 (45) | 36 (47) | 2 | ||
1: Manual agitation with glycine; 2: homogenization with magnetic stirrer and glycine; 3: manual agitation with Alconox®; 4: homogenization with magnetic stirrer and Alconox®; p1: Kruskal-Wallis analysis for dose and protocol used; p2: Kruskal-Wallis analysis for average and % of eggs recovered.
LK: Conceptualization, Data curation, Investigation, Methodology, Writing—original draft, Writing—review & editing, Formal analysis, Visualization. ECdM, MAMM, IARC, JVdA, MRB, and LSF: Data curation, Investigation, Writing—review & editing. DAGL: Conceptualization, Data curation, Investigation, Methodology, Writing—original draft, Writing—review & editing, Supervision, Formal analysis, Visualization. All authors read and approved the submitted version.
The authors declare that there are no conflicts of interest.
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The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.
This study did not receive external funding support.
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