All the authors of the present study declare no conflicts of interest.
Ethical approval
The exploitation of animals complies with the ethical guidelines established by the US National Institutes of Health (NIH). Ethical approval for animal use (FUTA/ETH/24/675) was obtained from the Animal Ethical Committee, Centre for Research and Development (CERAD), Federal University of Technology, Akure.
Consent to participate
Not applicable.
Consent to publication
Not applicable.
Availability of data and materials
The results section provides the summarized data necessary to support the conclusions of this manuscript. The raw data are available upon reasonable request from the corresponding author.
Funding
This study received no funding or financial support from any organization.
Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.
References
Russell C, Grimes C, Baker P, Sievert K, Lawrence MA. The drivers, trends and dietary impacts of non-nutritive sweeteners in the food supply: a narrative review.Nutr Res Rev. 2021;34:185–208. [DOI] [PubMed]
Akanji MA, Rotimi DE, Elebiyo TC, Awakan OJ, Adeyemi OS. Redox Homeostasis and Prospects for Therapeutic Targeting in Neurodegenerative Disorders.Oxid Med Cell Longev. 2021;2021:9971885. [DOI] [PubMed] [PMC]
Moriconi E, Feraco A, Marzolla V, Infante M, Lombardo M, Fabbri A, et al. Neuroendocrine and Metabolic Effects of Low-Calorie and Non-Calorie Sweeteners.Front Endocrinol (Lausanne). 2020;11:444. [DOI] [PubMed] [PMC]
Shaher SAA, Mihailescu DF, Amuzescu B. Aspartame Safety as a Food Sweetener and Related Health Hazards.Nutrients. 2023;15:3627. [DOI] [PubMed] [PMC]
Al-Okbi SY. Date Palm as Source of Nutraceuticals for Health Promotion: a Review.Curr Nutr Rep. 2022;11:574–91. [DOI] [PubMed] [PMC]
Mazi TA, Stanhope KL. Erythritol: An In-Depth Discussion of Its Potential to Be a Beneficial Dietary Component.Nutrients. 2023;15:204. [DOI] [PubMed] [PMC]
Muñoz-Labrador A, Hernandez-Hernandez O, Moreno FJ. A review of the state of sweeteners science: the natural versus artificial non-caloric sweeteners debate. Stevia rebaudiana and Siraitia grosvenorii into the spotlight.Crit Rev Biotechnol. 2024;44:1080–102. [DOI] [PubMed]
Spagnuolo MS, Mazzoli A, Nazzaro M, Troise AD, Gatto C, Tonini C, et al. Long-Lasting Impact of Sugar Intake on Neurotrophins and Neurotransmitters from Adolescence to Young Adulthood in Rat Frontal Cortex.Mol Neurobiol. 2023;60:1004–20. [DOI] [PubMed] [PMC]
Dar W. Aspartame-induced cognitive dysfunction: Unveiling role of microglia-mediated neuroinflammation and molecular remediation.Int Immunopharmacol. 2024;135:112295. [DOI] [PubMed]
Savaliya M, Georrge JJ. The monoaminergic system in humans.In: Recent Trends in Science and Technology-2020. Christ Publications; 2020. pp. 190–203. [DOI]
Ademosun AO, Popoola TV, Oboh G, Fasakin OW. Parquetina nigrescens and Spondias mombin protects against neurochemical alterations in the scopolamine model of cognitive dysfunction.J Food Biochem. 2022;46:e14213. [DOI] [PubMed]
Kępka A, Ochocińska A, Borzym-Kluczyk M, Chojnowska S, Skorupa E, Przychodzeń M, et al. Healthy Food Pyramid as Well as Physical and Mental Activity in the Prevention of Alzheimer’s Disease.Nutrients. 2022;14:1534. [DOI] [PubMed] [PMC]
Oguntuase SO, Ijarotimi OS, Oluwajuyitan TD, Oboh G. Nutritional, antioxidant, carbohydrate hydrolyzing enzyme inhibitory activities, and glyceamic index of wheat bread as influence by bambara groundnut substitution.SN Appl Sci. 2022;4:121. [DOI]
Fasakin OW, Awosika A, Ogunsanya ST, Benson IO, Olopoda AI. Anti-hypertensive effect of enriched white melon seed protein concentrate biscuit on sodium fluoride exposed rats.World J Exp Med. 2025;15:105798. [DOI] [PubMed] [PMC]
Oyeleye SI, Olasehinde TA, Fasakin OW, Oboh G, Saliu JA. Phyllanthus amarus Schumach. & Thonn. and Momordica charantia L extracts improve memory function, attenuate cholinergic and purinergic dysfunction, and suppress oxidative stress in the brain of doxorubicin–treated rats.Phytomed Plus. 2022;2:100283. [DOI]
Veilleux-Lemieux D, Beaudry F, Hélie P, Vachon P. Effects of endotoxemia on the pharmacodynamics and pharmacokinetics of ketamine and xylazine anesthesia in Sprague-Dawley rats.Vet Med (Auckl). 2012;3:99–109. [DOI] [PubMed] [PMC]
Oguntuase SO, Fasakin OW, Oyeleye IS, Oboh G. Functional Bread From Bambara Groundnut and Orange Peel Attenuates Hyperglycemia and Oxidative Stress in a High‐Fat Diet/Streptozotocin‐Induced Type 2 Diabetes Rat Model.Food Chem Int. 2026:1–11. [DOI]
Fasakin OW, Oboh G, Ademosun AO, Lawal AO. The modulatory effects of alkaloid extracts of Cannabis sativa, Datura stramonium, Nicotiana tabacum and male Carica papaya on neurotransmitter, neurotrophic and neuroinflammatory systems linked to anxiety and depression.Inflammopharmacology. 2022;30:2447–76. [DOI] [PubMed]
Green AL, Haughton TM. A colorimetric method for the estimation of monoamine oxidase.Biochem J. 1961;78:172–5. [DOI] [PubMed] [PMC]
Fasakin OW, Oboh G, Ademosun AO. Alkaloids-rich extracts from Cannabis sativa, Datura stramonium, and Nicotiana tabacum modulate sexual behavior and key enzymes relevant to sexual function in rats.Comp Clin Pathol. 2022;31:397–407. [DOI]
Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity.Biochem Pharmacol. 1961;7:88–95. [DOI] [PubMed]
Fasakin OW, Oboh G, Ademosun AO. Neuromodulatory evaluation of commonly abused plants ex vivo: a comparative study.Comp Clin Path. 2021;30:671–80. [DOI]
Agunloye OM, Aijo AE, Fasakin OW, Oboh G. Efficacy of Lecaniodiscus cupanioide and Alchornea cordifolia supplemented diets on neurotransmitter, neuroinflammatory, and antioxidant systems of scopolamine-induced amnesia in rat hippocampus.Explor Neurosci. 2025;4:100690. [DOI]
Hayashi I, Morishita Y, Imai K, Nakamura M, Nakachi K, Hayashi T. High-throughput spectrophotometric assay of reactive oxygen species in serum.Mutat Res. 2007;631:55–61. [DOI] [PubMed]
Fasakin OW, Oboh G, Ademosun AO. The addictive and neurotoxicological evaluation of Cannabis sativa, male Carica papaya, Nicotiana tabacum and Datura stramonium alkaloid extracts via neuroinflammatory, neurotransmitter and oxidative systems.IBRO Neurosci Rep. 2024;19:1141–56. [DOI] [PubMed] [PMC]
Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase.J Biol Chem. 1972;247:3170–5. [PubMed]
Ademosun AO, Oboh G, Adebayo AA, Olasehinde TA, Fasakin OW. Grapefruit peel extract mitigates paroxetine-induced erectile dysfunction in rats through stimulation of erectile response, antioxidant status, and inhibition of key enzymes related with impaired penile erection.J Food Biochem. 2022;46:e14193. [DOI] [PubMed]
Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.J Biol Chem. 1974;249:7130–9. [PubMed]
Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as a component of glutathione peroxidase.Science. 1973;179:588–90. [DOI] [PubMed]
Charan J, Kantharia ND. How to calculate sample size in animal studies?J Pharmacol Pharmacother. 2013;4:303–6. [DOI] [PubMed] [PMC]
Contreras-Rodriguez O, Solanas M, Escorihuela RM. Dissecting ultra-processed foods and drinks: Do they have a potential to impact the brain?Rev Endocr Metab Disord. 2022;23:697–717. [DOI] [PubMed]
Asdaq SMB, Almutiri AA, Alenzi A, Shaikh M, Shaik MA, Alshehri S, et al. Unveiling the Neuroprotective Potential of Date Palm (Phoenix dactylifera): A Systematic Review.Pharmaceuticals (Basel). 2024;17:1221. [DOI] [PubMed] [PMC]
Grembecka M. Sugar alcohols as sugar substitutes in food industry. In: Mérillon JM, Ramawat K, editors. Sweeteners. Reference Series in Phytochemistry. Springer, Cham; 2018. pp. 547–73. [DOI]
Behl T, Kaur D, Sehgal A, Singh S, Sharma N, Zengin G, et al. Role of Monoamine Oxidase Activity in Alzheimer’s Disease: An Insight into the Therapeutic Potential of Inhibitors.Molecules. 2021;26:3724. [DOI] [PubMed] [PMC]
Witek K, Wydra K, Filip M. A High-Sugar Diet Consumption, Metabolism and Health Impacts with a Focus on the Development of Substance Use Disorder: A Narrative Review.Nutrients. 2022;14:2940. [DOI] [PubMed] [PMC]
Villeda-González JD, Gómez-Olivares JL, Baiza-Gutman LA. New paradigms in the study of the cholinergic system and metabolic diseases: Acetyl-and-butyrylcholinesterase.J Cell Physiol. 2024;239:e31274. [DOI] [PubMed]
Adefegha SA, Okeke BM, Oboh G. Antioxidant properties of eugenol, butylated hydroxylanisole, and butylated hydroxyl toluene with key biomolecules relevant to Alzheimer's diseases-In vitro.J Food Biochem. 2021;45:e13276. [DOI] [PubMed]
Zhang Q, Jin K, Chen B, Liu R, Cheng S, Zhang Y, et al. Overnutrition Induced Cognitive Impairment: Insulin Resistance, Gut-Brain Axis, and Neuroinflammation.Front Neurosci. 2022;16:884579. [DOI] [PubMed] [PMC]
Li F, Luo Q, Guo T, Zhou S, Cheng Z, Pan H, et al. The impact of high-sugar diets on central nervous system disorders: mechanisms, pathogenesis, and dietary implication.Ann Med. 2025;57:2561789. [DOI] [PubMed] [PMC]
Bacil GP, Romualdo GR, Rodrigues J, Barbisan LF. Indole-3-carbinol and chlorogenic acid combination modulates gut microbiome and attenuates nonalcoholic steatohepatitis in a murine model.Food Res Int. 2023;174:113513. [DOI] [PubMed]
Abdelghffar EA, Obaid WA, Mohammedsaleh ZM, Ouchari W, Eldahshan OA, Sobeh M. Ajwa dates (Phoenix dactylifera L.) attenuate cisplatin-induced nephrotoxicity in rats via augmenting Nrf2, modulating NADPH oxidase-4 and mitigating inflammatory/apoptotic mediators.Biomed Pharmacother. 2022;156:113836. [DOI] [PubMed]
Dubey P, Pandey KB. Neurotransmitter modulation by phytochemicals. In: Egbuna C, Rudrapal M, editors. Phytochemical Drug Discovery for Central Nervous System Disorders. John Wiley & Sons, Ltd; 2023. pp. 311–25. [DOI]
Echegaray N, Gullón B, Pateiro M, Amarowicz R, Misihairabgwi JM, Lorenzo JM. Date fruit and its by-products as promising source of bioactive components: A review.Food Rev Int. 2023;39:1411–32. [DOI]
Han JY, Park M, Lee HJ. Stevia (Stevia rebaudiana) extract ameliorates insulin resistance by regulating mitochondrial function and oxidative stress in the skeletal muscle of db/db mice.BMC Complement Med Ther. 2023;23:264. [DOI] [PubMed] [PMC]
Soetan OA, Ajao FO, Ajayi AF. Blood glucose lowering and anti-oxidant potential of erythritol: An in vitro and in vivo study.J Diabetes Metab Disord. 2023;22:1217–29. [DOI] [PubMed] [PMC]
