Specifications of the five Fuc-specific lectins [163]

Number Lectin Preferred binding Fuc Substances used for elution
1 Lens culinaris agglutinin Fucα1-6GlcNAc methyl α-D-mannoside
2 Lotus tetragonolobus lectin Fucα1-3GlcNAc, Fucα1-3 (Lewis X and Y), sLeX L-Fuc
3 Ulex europaeus lectin I Fucα1-2Gal β1-4Glc(NAc) L-Fuc
4 Aleuria aurantia lectin Fucα1-2, Fucα1-3/4, Fucα1-6GlcNAc L-Fuc
5 Aspergillus oryzae lectin Fucα1-2, Fucα1-3/4, Fucα1-6GlcNAc L-Fuc

Note. Adapted from “Comparison of fucose-specific lectins to improve quantitative AFP-L3 assay for diagnosing hepatocellular carcinoma using mass spectrometry,” by Lee J, Yeo I, Kim Y, Shin D, Kim J, Kim Y, et al. J Proteome Res. 2022;21:1548–57 (https://pubs.acs.org/doi/10.1021/acs.jproteome.2c00196). © 2022, American Chemical Society.