Specifications of the five Fuc-specific lectins 
|Number||Lectin||Preferred binding Fuc||Substances used for elution|
|1||Lens culinaris agglutinin||Fucα1-6GlcNAc||methyl α-D-mannoside|
|2||Lotus tetragonolobus lectin||Fucα1-3GlcNAc, Fucα1-3 (Lewis X and Y), sLeX||L-Fuc|
|3||Ulex europaeus lectin I||Fucα1-2Gal β1-4Glc(NAc)||L-Fuc|
|4||Aleuria aurantia lectin||Fucα1-2, Fucα1-3/4, Fucα1-6GlcNAc||L-Fuc|
|5||Aspergillus oryzae lectin||Fucα1-2, Fucα1-3/4, Fucα1-6GlcNAc||L-Fuc|
Note. Adapted from “Comparison of fucose-specific lectins to improve quantitative AFP-L3 assay for diagnosing hepatocellular carcinoma using mass spectrometry,” by Lee J, Yeo I, Kim Y, Shin D, Kim J, Kim Y, et al. J Proteome Res. 2022;21:1548–57 (https://pubs.acs.org/doi/10.1021/acs.jproteome.2c00196). © 2022, American Chemical Society.
QC: Conceptualization, Writing—original draft, Writing—review & editing. HL: Writing—original draft, Writing—review & editing. XL: Conceptualization, Writing—original draft, Writing—review & editing, Supervision.
The authors declare that they have no conflicts of interest.
The work was funded by the Laboratory for Synthetic Chemistry and Chemical Biology Limited under the Health@InnoHK Program by the Innovation and Technology Commission. The Funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
© The Author(s) 2023.