From:  Neural glial network instability in epilepsy

 From acute disturbance to chronic epilepsy.

Disease stage/instability phaseDominant processesNeural contributionGlial contributionTissue/Circuit consequenceClinical or translational relevance
Stable/Compensated networkBalanced excitation and inhibition, preserved extracellular homeostasis, effective buffering and recovery mechanisms, restrained inflammatory tone, adaptive plasticityNeurons maintain controlled firing, appropriate inhibitory restraint, and normal response to perturbationAstrocytes, microglia, and other glial cells support potassium buffering, glutamate clearance, inflammatory restraint, metabolic coupling, and synaptic stabilityNetwork remains resistant to hypersynchrony and can absorb transient perturbations without progressing to seizure-prone organizationRepresents the physiological reference state and highlights the stabilizing systems that later therapies may seek to preserve or restore [9, 82, 184]
Early destabilizationPartial weakening of inhibitory restraint, impaired buffering, altered neurotransmitter clearance, inflammatory priming, reduced recovery efficiencyIncreased responsiveness to perturbation, emerging excitability changes, less effective inhibitory containment, fluctuating thresholds for synchronized activityReduced astrocytic homeostatic efficiency, early microglial activation, beginning loss of inflammatory restraint, stress-sensitive glial signalingNetwork appears partly compensated but becomes more vulnerable to recurrent excitation and less able to return fully to baseline after stressIdentifies a potentially intervention-sensitive phase in which compensatory mechanisms still exist but are becoming less reliable [178]
Acute seizure-associated disturbanceIntense neuronal firing, extracellular ionic disruption, neurotransmitter imbalance, calcium overload, acute glial activation, tissue stress signalingPathological discharge, hypersynchrony, recruitment of seizure-relevant ensembles, acute excitatory–inhibitory breakdownAstrocytic buffering stress, microglial activation, inflammatory signaling, altered water and neurotransmitter regulationAcute instability, local and distributed network disruption, cellular stress, and increased risk that seizure activity will leave a biological imprintClinically relevant for seizure termination, injury limitation, and prevention of secondary destabilizing cascades [37, 185, 186]
Epileptogenic transitionSustained inflammatory signaling, danger-associated responses, blood–brain barrier dysfunction, maladaptive plasticity, incomplete recovery, repeated reactivation of destabilizing pathwaysPersistent threshold lowering, altered synaptic responsiveness, impaired recovery, reinforcement of pro-excitatory circuit behaviorChronic or repeated glial activation, inflammatory amplification, impaired homeostatic support, glia-mediated contribution to remodeling and persistent vulnerabilityAcute disturbances become consolidated into more durable seizure-prone states with impaired resilience and increased susceptibility to recurrenceRepresents a major translational target because it is the phase in which chronic epilepsy may be prevented or attenuated before remodeling becomes more fixed [169, 178]
Chronic remodeled epileptic networkSynaptic reorganization, mossy fiber sprouting where relevant, interneuron network alteration, rewiring after injury, persistent connectivity and synchrony changesNeuronal networks become chronically permissive for recruitment, propagation, and recurrent pathological synchronizationGlial signaling, inflammatory persistence, extracellular matrix remodeling, metabolic and calcium-linked support of maladaptive circuit organizationSeizure-proneness becomes embedded in tissue and circuit architecture, allowing instability to persist even between overt seizuresHighlights why long-standing epilepsy often requires network-level and mechanism-guided therapies rather than acute suppression alone [37, 167, 169]