From:  The “lactate window” hypothesis: exercise-induced lactate dynamics in neurometabolic remodeling and symptom-dimensional exercise prescription for depression

 Evidence map for lactate-related mechanisms relevant to exercise-based antidepressant research.

Mechanistic domainCore evidenceDepression-relevant inferenceEvidence strength and key limitationKey refs
Peripheral lactate kineticsExercise intensity, modality, training status, and recovery influence lactate peak, AUC, time-to-peak, and clearance.Links external exercise dose to internal metabolic response.Established exercise physiology; not depression-specific and influenced by nutrition, sleep, sex, age, fitness, medication, and metabolic health.[412]
Brain lactate uptakeHuman physiology and MRS studies show brain lactate uptake during exercise or elevated arterial lactate.Supports peripheral-central metabolic communication.Human physiological evidence; mostly non-depressed samples and not direct proof of antidepressant mechanism.[41, 5965]
MCT transportMCT1, MCT2, and MCT4 mediate lactate transport across BBB, astrocytes, and neurons.Provides a transport route for lactate movement between blood, glia, and neurons.Strong molecular/translational evidence; transporter expression and function may vary by region, disease state, and training.[29, 30, 41]
ANLS and glial-neuronal couplingAstrocyte-derived lactate supports neural activity, memory, and plasticity.Relevant to depression-related circuits requiring metabolic support during cognitive control and effort.Mechanistic evidence is substantial but direct clinical evidence in MDD remains limited.[20, 21, 27, 28, 3134, 7072]
Plasticity signalingLactate can influence BDNF, CREB, SIRT1, Arc, c-Fos, Zif268, and NMDA-related signaling.May support activity-dependent remodeling after exercise.Mostly preclinical/cellular evidence; direction and magnitude in human MDD are uncertain.[28, 3438]
HCAR1-VEGF neurovascular pathwayExercise-induced lactate can signal through HCAR1 to induce VEGF and angiogenesis in experimental models.Links exercise metabolism to vascular and neurogenic adaptation.Preclinical evidence; clinical relevance and dose-response in depression are unproven.[39]
Glial-immunometabolic regulationLactate affects microglia, inflammation, phagocytosis, and histone lactylation.May modulate neuroinflammatory aspects of fatigue and motivational symptoms.Context-dependent mechanisms; lactate can have divergent immune effects depending on concentration, duration, cell type, and disease state.[4346]
Antidepressant-like behaviorPeripheral or acute lactate administration produces antidepressant-like effects in animal models.Direct preclinical support for behavioral relevance.Animal evidence; cannot be interpreted as direct clinical antidepressant efficacy.[40, 74]
Lactate paradoxRegional dmPFC/dACC lactate is associated with physical effort-based decision-making in humans.Warns against the claim that more lactate is always beneficial.Human neuroimaging evidence is correlational; directionality, task demand, and confounding remain unresolved.[73]
Translational exercise dosingExercise physiology studies show that blood lactate responses vary with exercise intensity, modality, training status, and recovery.We propose that lactate kinetics be prospectively evaluated as candidate physiological feedback variables for characterizing internal metabolic response, tolerability, and putative responder phenotypes in future MDD exercise trials.Hypothesis-generating only; prospective validation in MDD is required before lactate-informed dosing can be considered a clinical prescription strategy.[412]

ANLS: astrocyte-neuron lactate shuttle; AUC: area under the curve; BBB: blood-brain barrier; BDNF: brain-derived neurotrophic factor; CREB: cAMP response element-binding protein; dACC: dorsal anterior cingulate cortex; dmPFC: dorsomedial prefrontal cortex; HCAR1: hydroxycarboxylic acid receptor 1; MCT: monocarboxylate transporter; MDD: major depressive disorder; MRS: magnetic resonance spectroscopy; NMDA: N-methyl-D-aspartate; SIRT1: sirtuin 1; VEGF: vascular endothelial growth factor.