From:  Innovations in hydrocephalus modeling: bridging animal models, bioengineering platforms, and precision therapies

 Treatment approaches for hydrocephalus.

Therapy typeDescriptionCurrent statusAdvantagesChallengesReferences
Surgical & shuntVPS, ETV ± CPC, antibiotic catheters, programmable valvesClinical useEffective CSF diversion, reduced shunt dependence, and infection reductionInfection, revision, and mechanical complexity[2934, 105, 106]
PharmacologicalAcetazolamide, furosemide, NKCC1 inhibitors, antifibroticsPreclinical/Limited clinicalNon-invasive, adjunct therapyLimited efficacy, systemic toxicity[108110]
Regenerative & cell-freeStem cells, MSCs, EVs, ependymal reprogramming (GemC1/Mcidas)PreclinicalRepair, neuroprotection, and restore the ependymal liningDelivery, tumorigenicity, and scalability[91, 113, 115]
Gene & molecularCRISPR/Cas9, AAV vectors, molecular targets (NKCC1, TRPV4)Preclinical/ExperimentalTargeted therapy, disease-modifyingImmune responses, delivery challenges[117119]
Modeling platformsOrganoids, organ-on-chip, computational modelsPreclinical/RegulatoryPersonalized testing, patient-specific modelingStandardization, reproducibility[35, 39, 83, 84, 88]

This table summarizes proven and newly emerging strategies, including surgical and shunt therapies (VPS, ETV, ETV-CPC, programmable/antibiotic valves), drug therapies (acetazolamide, furosemide, NKCC1 inhibitors, antifibrotics), regenerative and cell-free therapies (stem cells, EVs, ependymal reprogramming), molecular and gene therapies (CRISPR/Cas9, AAV vectors, ion channel targets), and bioengineered modeling platforms (organoids, organ-on-chip, computational fluid dynamics). Each entry clarifies therapeutic rationale and status. AAV: adeno-associated virus; CPC: choroid plexus cauterization; CSF: cerebrospinal fluid; ETV: endoscopic third ventriculostomy; EVs: extracellular vesicles; MSCs: mesenchymal stem cells; NKCC1: Na+-K+-2Cl cotransporter 1; SVZ: subventricular zone; VPS: ventriculoperitoneal shunt; TRPV4: transient receptor potential vanilloid 4.