Table Info

Table 1.

List of necessary primary antibodies, reagents, instruments and software

Resource	Company	Clone	Concentration	Catalogue number
Antibody
CD3e	Invitrogen	SP7	n.d.¹	MA5-14524
Pan-γδ	Santa Cruz Biotechnology	H-41	0.1 mg/mL	Sc-100289
Granzyme B	Cell Signaling	D6E9W	6 μg/mL	#46890
BTN2A1	Atlas Antibodies	Polyclonal	50 μg/mL	HPA019208
BTN3A1	Antibodies Online	4E1B9	0.1 mg/mL	ABIN5684130
PanCK	Abcam	AE1/AE3	80 μg/mL	Ab27988
Melanoma Cocktail²	Abcam	HMB45 + M2-7C10 + M2-9E3	n.d.	Ab732
Reagent
Manual Opal™ 7-Color IHC Kit³	Akoya Biosciences			NEL811001KT
VECTASHIELD Vibrance™	Abacus dx			VEH170010
Instrument
Vectra^® 3 Automated	Akoya Biosciences
Quantitative Pathology Imaging System
Software
inForm^® Tissue Analysis Software	Akoya Biosciences
HALO^® Image Analysis Platform	Indica Labs

not determined (supplied as cell culture supernatant); 2

this cocktail includes HMB45 and MART-1; 3

includes antigen retrieval (AR) buffer at pH6 (AR6 buffer), AR9 buffer, antibody diluent and blocking buffer formulation (Blocking/Antibody Diluent), Opal™ Polymer horseradish peroxidase (HRP) mouse and rabbit (reacts specifically with primary antibodies raised in mouse and rabbit), Opal™ signal amplification diluent (1× Plus Amplification Diluent), 6 reactive Opal™ fluorophores (Opal™ 520, 540, 570, 620, 650 and 690), and spectral DAPI

Declarations

Acknowledgments

The Olivia Newton-John Cancer Research Institute acknowledges the support of the Victorian Government Operational Infrastructure Support Program, and the Ian Potter Foundation for providing funds to purchase the Vectra System. We would also like to thank Dr Khashaya Asadi, Austin Pathologist, for the provision of lung cancer FFPE tissue slides.

Author contributions

JDGD, LTQ, ET, SO and AB contributed to the concept, design and execution of the method; JDGD wrote the original manuscript; LTQ, SO and AB reviewed and edited the manuscript; JDGD and AB supervised the project; AB acquired funding for the project. All authors have read and agreed to the published version of the manuscript.

Conflicts of interest

AB is the recipient of research funding for work on butyrophilins in cancer; AB and SO are inventors on multiple patents related to the topic. No other authors declare a conflict of interest.

Ethical approval

Not applicable.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

Not applicable.

Funding

JDGD is supported by Cure Cancer Australia through the Cancer Australia Priority—Driven Cancer Research Scheme (#1187815). The contents of the published material are solely the responsibility of La Trobe University and do not reflect the views of Cancer Australia. AB is the recipient of a Fellowship from the Victorian Government Department of Health and Human Services acting through the Victorian Cancer Agency. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

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