Exploration of Immunology

Table 4. Allelic frequency and genotyping frequency association of the TLR4 Gene.

(rs1927914) Locus G>A	TLR4	Abortion (n, %)	Pregnant (n, %)	X²	P_(HWE)	OR (95% CI)	P- value
Alleles	A	123 (61.5%)	118 (59%)	0.261	0.609	Ref.
	G	77 (38.5%)	82 (41%)			0.901 (0.603–1.345)	0.610
	Total	200 (100%)	200 (100%)			0.901 (0.603–1.345)	0.610
Codominant	AA	41 (41%)	29 (29%)	7.321	0.026^*	Ref.
	GA	41 (41%)	60 (60%)			0.483 (0.26–0.898)	0.021^*
	GG	18 (18%)	11 (11%)			1.157 (0.476–2.813)	0.747
	Total	100 (100%)	100 (100%)			1.157 (0.476–2.813)	0.747
Dominant	AA	41 (41%)	29 (29%)	3.165	0.075	Ref.
	GA-GG	59 (59%)	71 (71%)			0.588 (0.327–1.058)	0.076
	Total	100 (100%)	100 (100%)			0.588 (0.327–1.058)	0.076
Recessive	AA-GA	82 (82%)	89 (89%)	1.976	0.160	Ref.
	GG	18 (18%)	11 (11%)			1.776 (0.792–3.984)	0.163
	Total	100 (100%)	100 (100%)			1.776 (0.792–3.984)	0.163
Over-dominant	AA-GG	59 (59%)	40 (40%)	7.221	0.007^*	Ref.
	GA	41 (41%)	60 (60%)			0.463 (0.263–0.815)	0.008^*
	Total	100 (100%)	100 (100%)			0.463 (0.263–0.815)	0.008^*

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^* Significant differences at P-value < 0.05. P_(HWE): P-value of Hardy–Weinberg equilibrium.

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Declarations

Acknowledgments

The authors would like to extend their sincere thanks to all participants in the research. Especially the staff of Al-Batool Maternity and Children’s Teaching Hospital for facilitating the collection of samples and providing the laboratories.

Author contributions

NMK: Conceptualization, Investigation, Writing—original draft, Writing—review & editing, Visualization, Formal analysis. BJAT: Validation, Writing—review & editing, Supervision. AKG: Validation, Writing—review & editing, Supervision. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The research was approved by the College of Medicine Ethics Committee at the University of Babylon. The collection of samples received approval from the Research and Development Department of the Diyala and Babylon Health Directorates, in accordance with the Ministry of Health letter No. 8199, dated February 12, 2024. This study complies with the Declaration of Helsinki (2024).

Consent to participate

Informed consent to participate in the study was obtained from all participants.

Consent to publication

Not applicable.

Availability of data and materials

The datasets that support the findings of this study are available from the corresponding author upon reasonable request.

Funding

This research received no external funding.

Copyright

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Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.

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