From:  Protocol workflow for whole blood memory B cell phenotyping and vaccine-specific antibody quantification

 List of monoclonal antibodies, ELISA kits, vaccines, instruments, and software applied in this protocol.

ResourceCompanyVolumeCatalogue number
Monoclonal antibodies
CD19-PerCP-CY5.5BD Pharmingen™10 µL340951
IgD-PEBD Pharmingen™10 µL555779
IgM-BB515BD Pharmingen™5 µL564622
CD24-APC-H7BD Pharmingen™5 µL658331
CD27-APCBD Pharmingen™5 µL337169
CD38-PE-Cy7BD Pharmingen™5 µL335790
ELISA kits
VaccZyme™ anti-tetanus toxoid IgGBinding Site, UK96-well plateMK010.0
VaccZyme™ anti-PCP IgGBinding Site, UK96-well plateMK012.0
Vaccines
Pneumovax23, PCP vaccineMerck Sharp & Dohme, Netherlands0.5 mL-
Tetanus toxoid vaccineBiofarma, Indonesia0.5 mL-
Instruments & software
Flow cytometer (FACS Lyrics)BD Biosciences, US--
BD FACS Suite softwareBD Biosciences, US--
FlowJo softwareBD Biosciences, US--
Microplate reader (Multiskan FC)Thermo Fisher Scientific, US--
Microplate reader software (SkanIt)Thermo Fisher Scientific, US--
Automated plate washer (Wellwash)Thermo Fisher Scientific, US--

PCP: pneumococcal polysaccharide.