From:  Features of peripheral blood Treg and Th17 subsets during physiological pregnancy

 Characteristics of monoclonal antibodies used for flow cytometry

Cellular markersFluorochromeCloneIsotypeManufactured
Panel 1
    CD4FITCOKT-4Mouse IgGb, κeBioscience
    CD25 (IL-2Rα)Brilliant Violet 605BC96Mouse IgG1, κBioLegend
    FOXP3PerCP-Cyanine5.5PCH101Rat IgG2a, κeBioscience
    RORγtAPCAFKJS-9Rat IgG2a, κeBioscience
    IL-17APerCP-Cyanine5.5eBio64DEC17Mouse IgG1, κeBioscience
    CD45RAAlexa Fluor 700HI100Mouse IgG2b, κBioLegend
    CD31Pacific BlueWM59Mouse IgG1, κBioLegend
    RAG2-3G4A26Mouse IgG2b, κBioLegend
    Anti-mouse IgG2bPERMG2b-1Rat IgG2a, κBioLegend
Panel 2
    CD4PET4Mouse IgG2b, κBioLegend
    CD25 (IL-2Rα)Brilliant Violet 605BC96Mouse IgG1, κBioLegend
    FOXP3PerCP-Cyanine5.5PCH101Rat IgG2a, κeBioscience
    RORγtPerCPNR1F3Mouse IgG1, κR&D Systems
    IL-17AFITCBL168Mouse IgG1, κBioLegend
    Tim-3 (CD366)APCF38-2E2Mouse IgG1, κBioLegend

FOXP3: X-linked Foxhead box P3