ROC curve analysis of IL-6, IL-10, and IL-6/IL-10 ratio for differentiating NRPL vs. RPL and 2 vs. > 2 pregnancy losses
| Parameters | NRPL vs. RPL | 2 losses vs. > 2 losses | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| AUC | Cut-off | Sn% | Sp.% | p-value | AUC | Cut-off | Sn% | Sp.% | p-value | |
| IL-6 | 0.992 | > 5.59 | 100 | 98.82 | 0.0001 | 0.502 | > 6.64 | 46.88 | 66.04 | 0.974 |
| IL-10 | 0.789 | < 14.08 | 100 | 63.53 | 0.0001 | 0.589 | > 9.58 | 28.13 | 92.45 | 0.170 |
| IL-6/IL-10 | 0.982 | > 0.62 | 96.47 | 92.94 | 0.0001 | 0.524 | < 0.97 | 43.75 | 69.81 | 0.703 |
IL-6: interleukin-6; RPL: recurrent pregnancy loss; NRPL: non-recurrent pregnancy loss; 2: two; > 2: more than two; AUC: area under the curve; Sn%: sensitivity; Sp.%: specificity; p < 0.05: statistically significant. Unit for IL-6, IL-10: pg/mL
We are grateful to Dr. Rajeshwari Bonu, Gynaecologist, Niloufer Government Maternity Hospital, Hyderabad, India, for giving us the opportunity to carry forward our research by providing blood samples, and we express gratitude to the blood donors for their cooperation in sharing their clinical data.
SJ: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing—original draft, Writing—review & editing. RB: Data curation, Investigation. PJ: Conceptualization, Project administration, Supervision, Funding acquisition, Resources, Formal analysis, Writing—review & editing.
The authors declare that they have no conflicts of interest.
The present study has approval from the Ethical Committee (Reg. No. ECR/300/Inst/AP/2013/RR-16), Osmania Medical College, Hyderabad, Telangana state, India, and informed consent from the study subjects. This study complies with the 2013 version of the Declaration of Helsinki.
Informed consent to participate in the study was obtained from all participants.
Not applicable.
The preprint for this study is available on Authorea. The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.
The authors have received research funding from the Indian Council of Medical Research, New Delhi, India [ICMR, Id No.: 2019–1022]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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