Methods to analyze T cell responses using moDCs as stimulatory cells in an MLR
| Responder cells | Source of moDCs | Days of stimulation | Proliferation method | Application | Reference and year |
|---|---|---|---|---|---|
| Peripheral blood CD4+ and CD8+ T cells purified by MACS | Peripheral blood CD14+ monocytes purified by MACS | 5 days | CD25 and Ki-67 expression | To evaluate phenotypic changes associated with lymphocyte responsiveness | [9]2024 |
| Peripheral blood naïve T cells purified by MACS | Peripheral blood CD14+ monocytes purified by MACS | 7 days | CFSE dilution | To evaluate the effect of Sulfavant A in an MLR | [10]2023 |
| PBMC | Monocytes from leukapheresis products | 5 days | CFSE dilution | To evaluate recipient T cell responses facing donor-derived moDC before liver transplantation | [11]2021 |
| Peripheral blood CD4+ and CD8+ T cells purified by MACS | Peripheral blood CD14+ monocytes purified by MACS | 5 days | WST-8/CCK8 tetrazolium assay | To evaluate the effect of IL-35 on moDCs differentiation and stimulatory capability | [12]2018 |
| Peripheral blood CD4+CD45RA– T cells purified by MACS | Peripheral blood CD14+ monocytes purified by MACS | 5 days | CFSE dilution | To analyze tolerance induction on effector/memory T cells | [13]2010 |
| Peripheral blood CD2+ T cells purified by MACS | Peripheral blood CD14+ monocytes purified by MACS | 4 days | [3H]-thymidine | To assess theimpact of stably immature, donor-derived DC on alloimmune reactivity | [119]2007 |
| CD4+ peripheral blood T cells purified by MACS | Peripheral blood CD14+ monocytes purified by MACS | 7 days | CFSE dilution and [3H]-thymidine | To evaluate alloreactive T-cell responses before and after depletion of alloantigen-specificT cells | [14]2004 |
| CD3+ cells obtained from the peripheral blood | Peripheral blood CD14+ monocytes purified by MACS | 5 days | [3H]-methyl-thymidine | To monitor immunological and clinical response to DC vaccination | [15]2004 |
moDCs: monocyte-derived DCs; MLR: mixed leukocyte reaction; MACS: magnetic cell sorting; CFSE: carboxy fluorescein succinimidyl ester; 3H: tritium; DC: dendritic cells; PBMC: peripheral blood mononuclear cells
The authors thank the Laboratorio Nacional de Citometría (LabNalCit), Oaxaca for their support for the moDCs-based MLR analysis from our laboratory, which has provided results for the writing of this review. During the preparation of this work, the authors utilized GitMind AI to create the hand elements of Figure 1. After using the tool, authors reviewed and edited the content as needed and will take full responsibility for the content of the publication.
HTA: Conceptualization, Funding acquisition. SASL, AAA, WdJRR: Data curation, Investigation, Writing—original draft. All authors have read and approved the submitted version.
The authors declare that they have no conflicts of interest.
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This research was funded by the Clinical and Basic Immunology Research Department Biochemical Sciences Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca. A.A.A. has doctoral fellowships of CONAHCyT number 799779. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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