Redox activity in newborn offspring with and without GDM and sucrose consumption
Oxidation/reduction parameters
Group A
Group B
Group C
Group D
P value
Mean ± SDn = 8
Mean ± SDn = 8
Mean ± SDn = 8
Mean ± SDn = 8
CP (nmol/mg protein)
12.47 ± 1.31
3.70 ± 0.46
44.91 ± 27.09a
23.10 ± 5.56
< 0.001
MDA (µM)
0.193 ± 0.086
0.171 ± 0.039
0.115 ± 0.074
0.139 ± 0.006
0.074
SOD (U/mL)
2.37 ± 0.93
2.98 ± 0.76
2.67 ± 0.92
1.82 ± 0.74
0.063
Catalase (U/L)
0.017 ± 0.002
0.015 ± 0.003
0.015 ± 0.002
0.012 ± 0.005a
< 0.050
RG (µM)
83.42 ± 6.57
67.27 ± 6.58
81.88 ± 20.53
121.06 ± 17a
< 0.001
TAC (µM eq. of Trolox)
0.404 ± 0.164
0.224 ± 0.236
0.607 ± 0.001a
0.469 ± 0.205
< 0.001
The values represent the mean ± standard deviation of the serum concentration of carbonylated proteins (CP), MDA, superoxide dismutase (SOD), catalase, reduced glutathione (RG) and total antioxidant capacity (TAC) with its units in µM eq. of Trolox. Twenty-four CD1 female mice were used to obtain the offspring (n = 6 per group). To process the samples from the offspring, groups of 8 newborns were created. Group A = without GDM and without sucrose supplementation, Group B = without GDM with sucrose supplementation, Group C = with GDM without sucrose supplementation, Group D = with GDM and with sucrose supplementation. The one-way ANOVA test was performed to compare the means between groups, with Tukey’s post hoc test to identify intergroup differences a with group A. P value < 0.05 was considered statistically significant
Declarations
Author contributions
BEMC: Conceptualization, Investigation, Writing—original draft, Writing—review & editing. EAR, CÁRG, and AARA: Investigation, Writing—original draft, Writing—review & editing. RVR, RAJL, and ALGL: Validation, Writing—review & editing, Supervision. All authors read and approved the submitted version.
Conflicts of interest
The authors declare that they have no conflicts of interest.
Ethical approval
The protocol was approved by the Faculty’s Research Ethics Committee (CONBIOETICA-15-CEI-002-20210531). The ethical approval number is “009.2022”.
Consent to participate
Not applicable.
Consent to publication
Not applicable.
Availability of data and materials
The raw data supporting the conclusions in this manuscript will be made available by the authors to any qualified researcher without undue reservation.
Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.
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