Main laboratory exploration methods for autoantibodies associated with hemostasis disorders
| Laboratory testing | Assay principle | Measurement type |
|---|---|---|
| Functional assays | Inhibition of activity | Measurement of factor activity; mixing studies. |
| Titration of inhibitor titer (Bethesda method) | Incubation of a normal sample with serial dilutions of patient sample and testing residual activity | Dilution inhibiting 50% of activity (corresponds to 1 unit) |
| Flow cytometry | Detection of circulating autoantibodies through their binding to Plt or blood cell GPs | Detection of bound antibodies to specific GPs |
| ELISA | Autoantigen capture-based assays | Detection of autoantibody by ELISA |
| MAIPA | Immunocapture of Plt GP and reactive autoantibodies | Identification of specific anti-GP autoantibodies |
| Coombs test | Detection of cell-bound antibodies | Detection of global anti-cell autoantibodies |
JA: Conceptualization, Writing—original draft, Writing—review & editing.
JA is consultant for HYPHEN BioMed SAS.
Not applicable.
Not applicable.
Not applicable.
Not applicable.
Not applicable.
© The Author(s) 2024.