Determination of compounds of interest (g/kg). Effect of AE, EE, and US methods in the extraction of polyphenols for the broccoli by-product. Results are expressed as mean values of triplicates ± SD
Compound
AE
EE
UAE
Total polyphenols (g GAE/kg DM)
21.32 ± 0.42
20.95 ± 0.31
3.91 ± 0.09
Chlorogenic acid glycoside
2.26 ± 0.05
3.24 ± 0.06
1.36 ± 0.07
Caffeoylquinic acid
8.47 ± 0.20
9.07 ± 0.13
1.64 ± 0.03
Di caffeoylquinic acid
10.04 ± 0.24
7.99 ± 0.10
0.76 ± 0.01
Declarations
Author contributions
MDF and FL: Conceptualization, Investigation, Writing—original draft, Writing—review & editing. DQM and PG: Validation, Writing—review & editing, Supervision. All authors read and approved the submitted version.
Conflicts of Interest
The authors declare that they have no conflict of interest.
Ethical approval
Not applicable.
Consent to participate
Not applicable.
Consent to publication
Not applicable.
Availability of data and materials
Not applicable.
Funding
This research was funded by AGROMATTER project Establecimiento de una red cervera para el desarrollo de materiales técnicos altamente sostenibles derivados de subproductos o residuos, and de la industria agrícola y de las operaciones de conservación de espacios naturales [CER-20211013] from Ministerio de Ciencia e Innovación-CDTI, Spain. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Osorio LLDR, Flórez-López E, Grande-Tovar CD. The potential of selected agri-food loss and waste to contribute to a circular economy: applications in the food, cosmetic and pharmaceutical industries.Molecules. 2021;26:515. [DOI] [PubMed] [PMC]
Chiocchio I, Mandrone M, Tomasi P, Marincich L, Poli F. Plant secondary metabolites: an opportunity for circular economy.Molecules. 2021;26:495. [DOI] [PubMed] [PMC]
Din Shirahigue L, Ceccato-Antonini SR. Agro-industrial wastes as sources of bioactive compounds for food and fermentation industries.Cienc Rural. 2020;50:e20190857. [DOI]
Cruzado M, Cedrón JC. Nutracéuticos, alimentos funcionales y su producción.Rev Quím. 2012;26:1–2. Spanish.
Reynoso-Camacho R, Rodríguez-Villanueva LD, Sotelo-González AM, Ramos-Gómez M, Pérez-Ramírez IZ. Citrus decoction by-product represents a rich source of carotenoid, phytosterol, extractable and non-extractable polyphenols.Food Chem. 2021;350:129239. [DOI] [PubMed]
Raiola A, Errico A, Petruk G, Monti DM, Barone A, Rigano MM. Bioactive compounds in Brassicaceae vegetables with a role in the prevention of chronic diseases.Molecules. 2018;23:15. [DOI] [PubMed] [PMC]
De la Fuente B, López-García G, Máñez V, Alegría A, Barberá R, Cilla A. Antiproliferative effect of bioaccessible fractions of four Brassicaceae microgreens on human colon cancer cells linked to their phytochemical composition.Antioxidants. 2020;9:368. [DOI] [PubMed] [PMC]
Bahadoran Z, Tohidi M, Nazeri P, Mehran M, Azizi F, Mirmiran P. Effect of broccoli sprouts on insulin resistance in type 2 diabetic patients: a randomized double-blind clinical trial.Int J Food Sci Nutr. 2012;63:767–71. [DOI] [PubMed]
Porter Y. Antioxidant properties of green broccoli and purple-sprouting broccoli under different cooking conditions.Biosci Horiz Int J Stud Res. 2012;5:hzs004. [DOI]
Ares AM, Nozal MJ, Bernal J. Extraction, chemical characterization and biological activity determination of broccoli health-promoting compounds.J Chromatogr A. 2013;1313:78–95. [DOI] [PubMed]
Jang HW, Moon JK, Shibamoto T. Analysis and antioxidant activity of extracts from broccoli (Brassica oleracea L.) sprouts.J Agric Food Chem. 2015;63:1169–74. [DOI] [PubMed]
Owis AI. Broccoli; the green beauty: a review.J Pharm Sci Res. 2015;7:696–703.
Dang Y, Zhou T, Hao L, Cao J, Sun Y, Pan D. In vitro and in vivo studies on the angiotensin-converting enzyme inhibitory activity peptides isolated from broccoli protein hydrolysate.J Agric Food Chem. 2019;67:6757–64. [DOI] [PubMed]
Jeffery EH, Araya M. Physiological effects of broccoli consumption.Phytochem Rev. 2009;8:283–98. [DOI]
Moreno DA, Garcia-Viguera C. El brócoli fuente de ingredientes funcionales: glucosinolatos.Aliment Nutricion y Salud. 2008;15:49–53. Spanish.
Rodríguez García SL, Raghavan V. Microwave-assisted extraction of phenolic compounds from broccoli (Brassica oleracea) stems, leaves, and florets: optimization, characterization, and comparison with maceration extraction.Recent Prog Nutr. 2022;2:1. [DOI]
Reche C, Rosselló C, Umaña MM, Eim V, Simal S. Mathematical modelling of ultrasound-assisted extraction kinetics of bioactive compounds from artichoke by-products.Foods. 2021;10:931. [DOI] [PubMed] [PMC]
Fernandes FA, Heleno SA, Pinela J, Carocho M, Prieto MA, Ferreira ICFR, et al. Recovery of citric acid from citrus peels: ultrasound-assisted extraction optimized by response surface methodology.Chemosensors. 2022;10:257. [DOI]
Ferreira SS, Passos CP, Cardoso SM, Wessel DF, Coimbra MA. Microwave assisted dehydration of broccoli by-products and simultaneous extraction of bioactive compounds.Food Chem. 2018;246:386–93. [DOI] [PubMed]
Saifullah M, McCullum R, McCluskey A, Vuong Q. Comparison of conventional extraction technique with ultrasound assisted extraction on recovery of phenolic compounds from lemon scented tea tree (Leptospermum petersonii) leaves.Heliyon. 2020;6:e03666. [DOI] [PubMed] [PMC]
Lu SY, Chu YL, Sridhar K, Tsai PJ. Effect of ultrasound, high-pressure processing, and enzymatic hydrolysis on carbohydrate hydrolyzing enzymes and antioxidant activity of lemon (Citrus limon) flavedo.LWT. 2021;138:110511. [DOI]
Barbosa PPM, Ruviaro AR, Martins IM, Macedo JA, LaPointe G, Macedo GA. Enzyme-assisted extraction of flavanones from citrus pomace: obtention of natural compounds with anti-virulence and anti-adhesive effect against Salmonella enterica subsp. enterica serovar Typhimurium.Food Control. 2021;120:107525. [DOI]
Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent.Meth Enzymol. 1999;299:152–78. [DOI]
Velasco P, Francisco M, Moreno DA, Ferreres F, García-Viguera C, Cartea ME. Phytochemical fingerprinting of vegetable Brassica oleracea and Brassica napus by simultaneous identification of glucosinolates and phenolics.Phytochem Anal. 2011;22:144–52. [DOI] [PubMed]
Álvarez R, Araya H, Navarro-Lisboa R, Lopez de Dicastillo C. Evaluation of polyphenol content and antioxidant capacity of fruits and vegetables using a modified enzymatic extraction.Food Technol Biotechnol. 2016;54:462–7. [DOI] [PubMed] [PMC]
Rajha HN, Abi-Khattar AM, El Kantar S, Boussetta N, Lebovka N, Maroun RG, et al. Comparison of aqueous extraction efficiency and biological activities of polyphenols from pomegranate peels assisted by infrared, ultrasound, pulsed electric fields and high-voltage electrical discharges.Innovative Food Sci Emerging Technol. 2019;58:102212. [DOI]
Xiong LG, Chen YJ, Tong JW, Huang JA, Li J, Gong YS, et al. Tea polyphenol epigallocatechin gallate inhibits Escherichia coli by increasing endogenous oxidative stress.Food Chem. 2017;217:196–204. [DOI] [PubMed]
Lou Z, Wang H, Zhu S, Ma C, Wang Z. Antibacterial activity and mechanism of action of chlorogenic acid.J Food Sci. 2011;76:M398–403. [DOI] [PubMed]
Mendoza-Isaza NA, Hoyos-Arbeláez JA, Peláez-Jaramillo CA. Capacidad antioxidante y contenido de polifenoles totales de extractos de tallo de Stevia rebaudiana en varios modelos in vitro.Rev EIA. 2020;17:34. Spanish. [DOI]
López-Hernández AA, Ortega-Villarreal AS, Vázquez Rodríguez JA, López-Cabanillas Lomelí M, González-Martínez BE. Application of different cooking methods to improve nutritional quality of broccoli (Brassica oleracea var. italica) regarding its compounds content with antioxidant activity.Int J Gastron Food Sci. 2022;28:100510. [DOI]
