From:  Diagnostic performance and cutoff optimisation of the CTD Screen assay for ANA detection compared with HEp-2 IFA

 Distribution of HEp-2 IFA patterns and their concordance with CTD Screen results.

HEp-2 IFACTD Screen
TypePatternIntensityPositives number (%)Positive number (%)Equivocal number (%)Negative number (%)
Nuclear
(n = 225)
Fine speckled2+/3+/4+89 (25.6%)55 (15.9%)4 (1.2%)30 (8.6%)
Homogeneous2+/3+/4+57 (16.4%)31 (8.9%)2 (0.6%)24 (6.9%)
Large speckled2+/3+/4+27 (7.8%)25 (7.2%)02 (0.6%)
Centromere2+/3+16 (4.6%)14 (4.0%)02 (0.6%)
Nucleolar homogeneous2+/3+12 (3.5%)3 (0.9%)2 (0.6%)7 (2.0%)
Dense fine speckled2+/3+/4+6 (1.7%)2 (0.6%)04 (1.2%)
Topo I3+5 (1.4%)5 (1.4%)00
Multiple discrete dots3+/4+4 (1.2%)2 (0.6%)02 (0.6%)
Pleomorphic2+/3+3 (0.9%)2 (0.6%)01 (0.3%)
Nucleolar punctate3+2 (0.6%)002 (0.6%)
Nucleolar clumpy3+2 (0.6%)002 (0.6%)
Envelope2+1 (0.3%)001 (0.3%)
Cytoplasmic
(n = 117)
Dense fine speckled2+/3+/4+32 (9.2%)31 (8.9%)1 (0.3%)9 (2.6%)
Fine speckled 2+/3+4 (1.2%)3 (0.9%)1 (0.3%)15 (4.3%)
Discrete dots2+/3+5 (1.4%)4 (1.2%)1 (0.3%)3 (0.9%)
Fibrillary, filamentous 2+/3+5 (1.4%)4 (1.2%)1 (0.3%)8 (2.3%)
Golgi1+/2+7 (2.0%)5 (1.4%)2 (0.6%)2 (0.6%)
Reticular2+/3+/4+10 (2.9%)7 (2.0%)3 (0.9%)13 (3.7%)
Fibrillary, linear2+/3+1 (0.3%)1 (0.3%)-1 (0.3%)
Rods & rings2+/3+1 (0.3%)1 (0.3%)-2 (0.6%)
Mitotic
(n = 5)
Centrosome2+/4+2 (0.6%)2 (0.6%)-1 (0.3%)
Intercellular bridge2+1 (0.3%)1 (0.3%)--
Chromosomal2+---1 (0.3%)

CTD Screen: connective tissue disease Screen; HEp-2 IFA: HEp-2 cell indirect immunofluorescence assay.