From:  Diagnostic performance and cutoff optimisation of the CTD Screen assay for ANA detection compared with HEp-2 IFA

 A comparison of CTD Screen results with HEp-2 IFA and LIA.

HEp-2 IFA patternsHEp-2 IFALIACTD Screen
PositivePositiveNegativePositiveEquivocalNegative
Total347228 (65.7%)119 (34.3%)198 (57.1%)17 (4.9%)132 (38.0%)
Nuclear225 (64.8%)159 (45.8%)67 (19.3%)139 (40.1%)8 (2.3%)77 (22.2%)
Cytoplasmic117 (33.7%)66 (19.0%)50 (14.4%)56 (16.1%)9 (2.6%)53 (15.3%)
Mitotic5 (1.4%)3 (0.9%)2 (0.6%)3 (0.9%)02 (0.6%)

CTD Screen: connective tissue disease Screen; HEp-2 IFA: HEp-2 cell indirect immunofluorescence assay; LIA: line immunoassay.