From:  Diagnostic performance and cutoff optimisation of the CTD Screen assay for ANA detection compared with HEp-2 IFA

 Disease association with different patterns in HEp-2 IFA-positive patients.

DiagnosisType of HEp-2 IFA patterns numbers (%)Specific ANA found in LIA (%)
NuclearCytoplasmicMitoticTotalSSA52KdSSA60KdU1RNPSmRibo-PScl-70SSB/LaCENP-BdsDNAAMA-M2HistoneNucleosomePCNAJo-1PM-Scl
SLE58 (25.8)21 (17.9)1 (20)80 (23.1)9.511.111.115.920.601.61.614.306.37.9000
SjS21 (9.3)17 (14.5)1 (20)39 (11.2)38.234.43.1000.8132.303.81.501.51.50
MCTD23 (10.2)10 (8.5)033 (9.5)14.610.921.216.1664.42.92.26.63.62.93.62.91.50
LSSc26 (11.6)0026 (7.5)8.88.85.902.944.12.920.60002.9002.9
DSSc9 (4.0)2 (1.7)1 (20)12 (3.5)012.512.50031.202506.26.206.206.2
Overlap syndrome15 (6.7)11 (9.4)026 (7.5)23.223.25.87.24.32.98.72.94.35.88.74.301.42.9
UCTD5 (2.2)005 (1.4)16.716.716.716.700033.30000000
RA16 (7.1)5 (4.3)021 (6.1)36.427.39.1009.1000009.1000
Others51 (22.7)52 (44.4)2 (40)105 (30.3)20209.1731.801.803.618.21.85.57.31.81.8

ANA: antinuclear antibody; DSSc: diffuse systemic sclerosis; HEp-2 IFA: HEp-2 cell indirect immunofluorescence assay; LIA: line immunoassay; LSSc: limited systemic sclerosis; MCTD: mixed connective tissue disease; RA: Rheumatoid arthritis; SjS: Sjogren’s syndrome; SLE: systemic lupus erythematosus; UCTD: undifferentiated connective tissue disease.