Spearman’s rank correlation of cytokines and antibodies at post-infestation days
Cytokines/Antibodies
N
Mean
TNF-α
IFN-γ
IL-4
IL-10
IgE
IgG
TNF-α
16
192.931
1
IFN-γ
16
389.6611
0.636**
1
IL-4
16
309.0694
0.548**
0.100
1
IL-10
16
340.2647
–0.369
–0.518**
0.739**
1
IgE
16
154.469
0.774**
0.246
0.896**
0.444**
1
IgG
16
245.295
0.479**
0.094
0.791**
0.241
0.626**
1
** Correlation is significant at the 0.01 level (2-tailed)
Declarations
Acknowledgments
We are grateful to Simon Masinde Kasili and Maurice Ndiema for the phlebotomy expertise in the withdrawal of blood from the animal. Linet Kim, Elisha Kimutai, Willy Aganyanya, and Lynn Omari for the technical assistance. We are also indebted to Evelyn Angariuki, Florence Avugwi, and Joram Omuoma for coordinating and providing their homes as the site of infestation.
Author contributions
JT: Conceptualization, Methodology, Validation, Data curation, Investigation, Writing—original draft, Writing—review & editing. JM: Conceptualization, Investigation, Resources, Writing—review & editing, Supervision, Funding acquisition. DMWO and MO: Funding acquisition. KK: Investigation, Methodology, Validation, Data curation. NK: Investigation, Writing—review & editing. JC: Conceptualization, Investigation, Writing—review & editing. All authors have read and agreed to the published version of the manuscript.
Conflicts of interest
The authors declare that they have no conflicts of interest.
Ethical approval
The experimental procedure was done in accordance with regulations of the Institutional Scientific and Ethical Review Committee of Masinde Muliro University of Science and Technology (MMUST/IERC/172/2023), and the National Commission for Science Technology and Innovation granted the licence for the study (NACOSTI/P/23/26624).
Consent to participate
Not applicable.
Consent to publication
Not applicable.
Availability of data and materials
All relevant data are available from the corresponding author on request [jcheruiyot@mmust.ac.ke].
Funding
The study was partially supported by Kenya National Research Fund Grants (NRF), reference number: [NRF/GRANT/VOL III/55]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.
References
Abrha S, Heukelbach J, Peterson GM, Christenson JK, Carroll S, Kosari S, et al. Clinical interventions for tungiasis (sand flea disease): a systematic review.Lancet Infect Dis. 2021;21:e234–45. [DOI] [PubMed]
Mutebi F, Krücken J, Feldmeier H, von Samsom-Himmelstjerna G. Clinical implications and treatment options of tungiasis in domestic animals.Parasitol Res. 2021;120:4113–23. [DOI] [PubMed]
Linardi PM. Fleas and Diseases.In: Arthropod Borne Diseases. Cham: Springer; 2017. pp. 517–36. [DOI]
Silvestri V, Mushi V, Ngasala B. Tungiasis.In: Vascular Damage in Neglected Tropical Diseases. Cham: Springer; 2024. pp. 81–9. [DOI]
Deka MA. Mapping the Geographic Distribution of Tungiasis in Sub-Saharan Africa.Trop Med Infect Dis. 2020;5:122. [DOI] [PubMed] [PMC]
Deka MA, Morshed N. Geospatial Analysis of Tungiasis Disease Transmission Risk in East Africa. In: Braimah JA, Bisung E, Kuuire V, editors. Health Geography in Sub-Saharan Africa. Cham: Springer; 2023. pp. 177–93. [DOI]
Heukelbach J, Harvey TV, Calheiros CML. Tunga Spp. and Tungiasis in Latin America.In: Infectious Tropical Diseases and One Health in Latin America. Cham: Springer; 2022. pp. 151–68. [DOI]
Obebe OO, Aluko OO. Epidemiology of tungiasis in sub-saharan Africa: a systematic review and meta-analysis.Pathog Glob Health. 2020;114:360–9. [DOI] [PubMed] [PMC]
Nagy N, Abari E, D’Haese J, Calheiros C, Heukelbach J, Mencke N, et al. Investigations on the life cycle and morphology of Tunga penetrans in Brazil.Parasitol Res. 2007;101:233–42. [DOI] [PubMed]
Nwalozie R, Ezenwaka CO. Tungiasis: Biology, Life Cycle, Epidemiology, Diagnosis, Prevention, and Treatment.South Asian J Parasitol. 2023;6:83–93.
Mphande FA. Skin Disorders in Vulnerable Populations: Causes, Impacts and Challenges. Singapore: Springer; 2020. [DOI]
Leiferman KM, Peters MS. Eosinophil-Related Disease and the Skin.J Allergy Clin Immunol Pract. 2018;6:1462–82.e6. [DOI] [PubMed]
Feldmeier H, Heukelbach J, Eisele M, Sousa AQ, Barbosa LM, Carvalho CB. Bacterial superinfection in human tungiasis.Trop Med Int Health. 2002;7:559–64. [DOI] [PubMed]
Feldmeier H, Heukelbach J, Eisele M, Ribeiro R, Harms G, Mehlhorn H, et al. Investigations on the biology, epidemiology, pathology and control of Tunga penetrans in Brazil: III. Cytokine levels in peripheral blood of infected humans.Parasitol Res. 2003;91:298–303. [DOI] [PubMed]
Feldmeier H, Witt LH, Schwalfenberg S, Albuquerque Ribeiro R, Queiroz Cunha F, Harms G, et al. Investigations on the biology, epidemiology, pathology and control of Tunga penetrans in Brazil. V. Cytokine concentrations in experimentally infected Wistar rats.Parasitol Res. 2004;94:371–6. [DOI] [PubMed]
Galli SJ, Metz M, Starkl P, Marichal T, Tsai M. Mast cells and IgE in defense against lethality of venoms: Possible “benefit” of allergy.Allergo J Int. 2020;29:46–62. [DOI] [PubMed] [PMC]
Vega-López F, Ritchie S. Skin Tropical Infections and Dermatology in Travellers.In: Principles and Practice of Travel Medicine. John Wiley & Sons, Inc.; 2013. pp. 165–96. [DOI]
Wells B, Burgess ST, McNeilly TN, Huntley JF, Nisbet AJ. Recent developments in the diagnosis of ectoparasite infections and disease through a better understanding of parasite biology and host responses.Mol Cell Probes. 2012;26:47–53. [DOI] [PubMed]
Birck MM, Tveden-Nyborg P, Lindblad MM, Lykkesfeldt J. Non-Terminal Blood Sampling Techniques in Guinea Pigs.J Vis Exp. 2014e51982. [DOI] [PubMed] [PMC]
Diehl KH, Hull R, Morton D, Pfister R, Rabemampianina Y, Smith D, et al.; European Federation of Pharmaceutical Industries Association and European Centre for the Validation of Alternative Methods. A good practice guide to the administration of substances and removal of blood, including routes and volumes.J Appl Toxicol. 2001;21:15–23. [DOI] [PubMed]
Parasuraman S, Raveendran R, Kesavan R. Blood sample collection in small laboratory animals.J Pharmacol Pharmacother. 2010;1:87–93. [DOI] [PubMed] [PMC]
Osorio-Pinzon J, Palencia A, Cruz-Calderon S, Rodriguez-Morales AJ. Myiasis and Tungiasis.Curr Trop Med Rep. 2021;8:112–20. [DOI]
Padilla-Carlin DJ, McMurray DN, Hickey AJ. The guinea pig as a model of infectious diseases.Comp Med. 2008;58:324–40. [PubMed] [PMC]
Feldmeier H, Eisele M, Van Marck E, Mehlhorn H, Ribeiro R, Heukelbach J. Investigations on the biology, epidemiology, pathology and control of Tunga penetrans in Brazil: IV. Clinical and histopathology.Parasitol Res. 2004;94:275–82. [DOI] [PubMed]
Liu X, Walton SF, Murray HC, King M, Kelly A, Holt DC, et al. Crusted scabies is associated with increased IL-17 secretion by skin T cells.Parasite Immunol. 2014;36:594–604. [DOI] [PubMed]
Mishra PK, Palma M, Bleich D, Loke P, Gause WC. Systemic impact of intestinal helminth infections.Mucosal Immunol. 2014;7:753–62. [DOI] [PubMed]
Bhat SA, Mounsey KE, Liu X, Walton SF. Host immune responses to the itch mite, Sarcoptes scabiei, in humans.Parasit Vectors. 2017;10:385. [DOI] [PubMed] [PMC]
Walton SF, Pizzutto S, Slender A, Viberg L, Holt D, Hales BJ, et al. Increased Allergic Immune Response to Sarcoptes scabiei Antigens in Crusted versus Ordinary Scabies.Clin Vaccine Immunol. 2010;17:1428–38. [DOI] [PubMed] [PMC]
Athanasiou LV, Spanou VM, Katsogiannou EG, Katsoulos PD. Hematological Features in Sheep with IgG and IgM Antibodies against Borrelia burgdorferi sensu lato.Pathogens. 2021;10:164. [DOI] [PubMed] [PMC]
