From:  Drug treatment of MASH from none to (too) many options?

 Recent randomized controlled clinical trials with new drugs in the MASH arena.

DrugMethodFindingsConclusionReference
TirzepatideA Phase 2 dose-finding, multicenter, double-blind RCT with 157 participants who had biopsy-proven MASH and stage F2 or F3 fibrosis. They were randomly assigned to receive either ow sc tirzepatide (5, 10, or 15 mg) or a placebo for 52 weeks.The proportion of participants who met the criteria for resolution of MASH without worsening of fibrosis was 10% in the placebo group, 44% in the 5 mg tirzepatide group (∆ vs. placebo, 34% points; 95% CI, 17 to 50), 56% in the 10 mg tirzepatide group (∆ 46% points; 95% CI, 29 to 62), and 62% in the 15 mg tirzepatide group (∆ 53% points; 95% CI, 37 to 69) (p < 0.001 for all three comparisons).
The proportion of participants who exhibited improvement of ≥ 1 fibrosis stage without worsening of MASH was 30% in the placebo group, 55% in the 5 mg tirzepatide group (∆ vs. placebo, 25% points; 95% CI, 5 to 46), 51% in the 10 mg tirzepatide group (∆, 22% points; 95% CI, 1 to 42), and 51% in the 15 mg tirzepatide group (∆, 21% points; 95% CI, 1 to 42). The most common adverse events in the tirzepatide groups were mostly mild or moderate GI events.
Tirzepatide administration for 52 weeks to participants with MASH and moderate or severe fibrosis was more effective than placebo in inducing MASH resolution without worsening fibrosis.Loomba et al., 2024 [16]
DenifanstatA Phase 2b multi-center, double-blind RCT including 168 participants who were randomized to receive either 50 mg denifanstat od (n = 112) or placebo (n = 56).38% of participants in the denifanstat group had a ≥ 2-point improvement in NAS without worsening of fibrosis versus nine (16%) of 56 participants in the placebo group (p = 0.0035). 26% of participants in the denifanstat group showed MASH resolution with a ≥ 2-point improved NAS without fibrosis worsening vs. 11% of participants in the placebo group (p = 0.0173). The most common treatment-emergent adverse events were COVID-19 (17% of those receiving denifanstat vs. 11% in the placebo group), ocular dryness, and hair loss.Denifanstat induced a significant improvement in NAS, MASH resolution, and fibrosis.Loomba et al., 2024 [17]
SemaglutideOngoing Phase 3 multicenter, double-blind RCT involving 1,197 subjects with biopsy-proven MASH and stage 2–3 fibrosis. They were assigned to receive either ow sc 2.4 mg semaglutide or a placebo for 240 weeks.MASH resolution without worsening of fibrosis was observed in 62.9% of those receiving semaglutide vs. 34.3% of those in the placebo group (p < 0.001). Reduced liver fibrosis without MASH worsening was found in 36.8% of those assigned to semaglutide vs. 22.4% of those receiving placebo (p < 0.001).Ow 2.4 mg semaglutide improved liver histology findings at week 72 among individuals with MASH and moderate or advanced liver fibrosis.Sanyal et al., 2025 [15]
Dapagliflozin54 adults with biopsy-proven MASH, with or without T2D, were randomly assigned to receive 10 mg of dapagliflozin orally or a matching placebo od for 48 weeks.MASH improvement without worsening of fibrosis was reported in 53% of those assigned to dapagliflozin vs. 30% receiving placebo (p = 0.006). The mean difference in NAS was also significant (p < 0.001). MASH resolution without worsening of fibrosis occurred in 23% of participants receiving dapagliflozin vs. 8% in the placebo group (p = 0.01). Fibrosis improvement without worsening of MASH was reported in 45% of participants treated with dapagliflozin vs. 20% of those receiving placebo (p = 0.001). The proportion of those who had adverse events leading to discontinued treatment was 1% with dapagliflozin vs. 3% with placebo.Compared to placebo, dapagliflozin administration for 48 weeks led to a higher percentage of individuals with MASH improvement without worsening fibrosis, as well as MASH resolution without worsening fibrosis and fibrosis improvement without worsening of MASH.Lin et al., 2025 [18]
EfruxiferminPhase 2b multicenter, double-blind, RCT including 126 participants who were assigned to receive sc efruxifermin (28 or 50 mg) once per week or a placebo.Of 88 participants with week-96 biopsies, ≥ 1-stage fibrosis improvement without MASH worsening was observed in 24% of 34 participants randomized to the placebo group, 46% of those receiving 28 mg (p = 0.070), and 75% of those in the 50 mg group (p < 0.0001). Mild-to-moderate GI adverse events were more common with efruxifermin than with placebo.After 96 weeks, treatment with efruxifermin resulted in greater improvements in fibrosis than placebo.Noureddin et al., 2025 [19]

CI: confidence interval; ∆: difference; GI: gastrointestinal; MASH: metabolic dysfunction-associated steatohepatitis; NAS: nonalcoholic fatty liver disease activity score; od: once daily; ow: once-weekly; RCT: randomized, placebo-controlled trial; sc: subcutaneous; T2D: type 2 diabetes.