Exploration of BioMat-X

From: Chitin from chicken bones and feet: reality or confusion? A brief analysis of the current situation

Table 1. Some of the FTIR signals used for chitin identification.

Signal (cm^–1)	Work reference									Average	± SD
Signal (cm^–1)	[19]	[20]	[21]	[21]	[22]	[23]	[24]	[25]	[26]	Average	± SD
O–H stretching	3,448	3,439	3,431	3,433	3,436	3,433	3,447	3,440	3,444	3,439	6
C–H stretching	2,883	2,885	2,889	2,882	2,877		2,890		2,891	2,885	5
Amide I (A)	1,660	1,662	1,653	1,656	1,649	1,652	1,652	1,660	1,660	1,656	5
Amide I (B)	1,627	1,630	1,622	1,622	1,621	1,621	1,619	1,627	1,623	1,624	4
Amide II (N–H flexion)	1,558	1,560	1,555	1,555	1,553	1,555	1,555	1,553		1,556	2
Amide III (C–N flexion)	1,312	1,319	1,308	1,312	1,307	1,308	1,310	1,312		1,311	4
C–O stretching	1,021	1,025	1,011	1,015	1,008	1,011	1,020			1,016	6

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References

Secher A. Travels with Trilobites: Adventures in the Paleozoic. New York: Columbia University Press; 2022.

Lárez-Velásquez C, López F. Chito-oligosaccharides: A mini-review on sources, production, and agricultural ap-plications. Adv Mod Agric. 2024;5:2730. [DOI]

Wasule DL, Shinde RM. Versatile Applications of Chitosan and Its Derivatives Across Diverse Industries. Biopolymers. 2026;117:e70073. [DOI] [PubMed]

Wu CC, Lai N, Chen BY, Hsueh CC. Feasibility study of chitosan extraction from waste leaves of Luffa cylindrica for bioresource recycling. Sust Chem Pharm. 2022;30:100864. [DOI]

Yahcob-Saddalani S, Pansacala J, Fernando J, Toledo R. Chitin extraction from sardine fish scales. Int J Biosci. 2022;21:5, 216–222. [DOI]

Jalal AF, Risheed CM, Ibrahim BM. Optimization of Chitin Extraction from Chicken Feet. J Anal Bioanal Techniques. 2012;03:05. [DOI]

Zaeed NA, Issa N, Karabet F. Preparation and characterization of chitosan from chicken feet. Am J Research. 2017;4:26–41.

Muhsin ZAA, Aldhamin AS, Shafik SS. Chicken Bone as Chitin Source. J Univ Shanghai Sci Technol. 2021;23:238–44.

Muhsin ZAA, Aldhamin AS, Shafik SS. Chitin Preparation and Characterization from Chicken Bone. Eco Env Cons. 2021;27:S150–6.

10.

National Center for Biotechnology Information - Gene database (NCBI Gene) [Internet]. National Library of Medicine; [cited 2026 Jan 19]. Available from: https://www.ncbi.nlm.nih.gov/gene/

11.

Genome Browser Gateway [Internet]. Genome Institute of the University of California, Santa Cruz; [cited 2026 Feb 13]. Available from: https://ucsc.crg.eu/cgi-bin/hgGateway

12.

Holen MM, Vaaje-Kolstad G, Kent MP, Sandve SR. Gene family expansion and functional diversification of chitinase and chitin synthase genes in Atlantic salmon (Salmo salar). G3 (Bethesda). 2023;13:jkad069. [DOI] [PubMed] [PMC]

13.

Stern R. Go Fly a Chitin: The Mystery of Chitin and Chitinases in Vertebrate Tissues. Front Biosci (Landmark Ed). 2017;22:580–95. [DOI] [PubMed]

14.

Weigel PH, West CM, Zhao P, Wells L, Baggenstoss BA, Washburn JL. Hyaluronan synthase assembles chitin oligomers with -GlcNAc(α1→)UDP at the reducing end. Glycobiology. 2015;25:632–43. [DOI] [PubMed] [PMC]

15.

DeAngelis PL, Zimmer J. Hyaluronan synthases; mechanisms, myths, & mysteries of three types of unique bifunctional glycosyltransferases. Glycobiology. 2023;33:1117–27. [DOI] [PubMed] [PMC]

16.

Sargent TD, Dawid IB. Differential gene expression in the gastrula of Xenopus laevis. Science. 1983;222:135–9. [DOI] [PubMed]

17.

Bulawa CE. CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: the CSD2 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis. Mol Cell Biol. 1992;12:1764–76. [DOI] [PubMed] [PMC]

18.

Atkinson EM, Long SR. Homology of Rhizobium meliloti NodC to polysaccharide polymerizing enzymes. Mol Plant Microbe Interact. 1992;5:439–42. [DOI] [PubMed]

19.

Cárdenas G, Cabrera G, Taboada E, Miranda SP. Chitin characterization by SEM, FTIR, XRD, and 13C cross polarization/mass angle spinning NMR. J App Polym Sci. 2004;93:1876–85. [DOI]

20.

Hajji S, Younes I, Ghorbel-Bellaaj O, Hajji R, Rinaudo M, Nasri M, et al. Structural differences between chitin and chitosan extracted from three different marine sources. Int J Biol Macromol. 2014;65:298–306. [DOI] [PubMed]

21.

Ibitoye EB, Lokman IH, Hezmee MNM, Goh YM, Zuki ABZ, Jimoh AA. Extraction and physicochemical characterization of chitin and chitosan isolated from house cricket. Biomed Mater. 2018;13:025009. [DOI] [PubMed]

22.

Kaya M, Sofi K, Sargin I, Mujtaba M. Changes in physicochemical properties of chitin at developmental stages (larvae, pupa and adult) of Vespa crabro (wasp). Carbohydr Polym. 2016;145:64–70. [DOI] [PubMed]

23.

Kaya M, Baran T, Asan-Ozusaglam M, Cakmak YS, Tozak KO, Mol A, et al. Extraction and characterization of chitin and chitosan with antimicrobial and antioxidant activities from cosmopolitan Orthoptera species (Insecta). Biotechnol Bioproc Eng. 2015;20:168–79. [DOI]

24.

Daraghmeh NH, Chowdhry BZ, Leharne SA, Al Omari MM, Badwan AA. Chitin. In: Brittain HG, editor. Profiles of Drug Substances, Excipients and Related Methodology, Vol. 36. Burlington: Academic Press; 2011. pp. 35–102.

25.

Aouadi A, Saoud DH, Laouini SE, Rebiai A, Achouri A, Mohammed HA, et al. Synergistic chitin-zinc nanocomposites from shrimp shell waste: characterization, antioxidant, and antibacterial properties. Biomass Conv Biorefin. 2025;15:545–61. [DOI]

26.

Focher B, Naggi A, Torri G, Cosani A, Terbojevich M. Structural differences between chitin polymorphs and their precipitates from solutions—evidence from CP-MAS 13C-NMR, FT-IR and FT-Raman spectroscopy. Carbohydr Polym. 1992;17:97–102. [DOI]

27.

Psarianos M, Rossi G, Van Der Borght M, Schlüter OK. Methods for estimating the chitin content of edible insects: Advantages and challenges. Carbohydr Polym. 2025;367:124009. [DOI] [PubMed]

28.

Cardozo FA, Facchinatto WM, Colnago LA, Campana-Filho SP, Pessoa A. Bioproduction of N-acetyl-glucosamine from colloidal α-chitin using an enzyme cocktail produced by Aeromonas caviae CHZ306. World J Microbiol Biotechnol. 2019;35:114. [DOI] [PubMed]

29.

Kumirska J, Czerwicka M, Kaczyński Z, Bychowska A, Brzozowski K, Thöming J, et al. Application of spectroscopic methods for structural analysis of chitin and chitosan. Mar Drugs. 2010;8:1567–636. [DOI] [PubMed] [PMC]

30.

Hemmami H, Zeghoud S, Amor IB, Ahmed S. Chitin and chitosan characterization: spectroscopic methods. In: Ahmed S, Zeghoud S, Hemmami H, Amor IB, editors. Chitin and Chitosan: Physical and Chemical Properties. Singapore: Jenny Stanford Publishing Pte. Ltd; 2025. pp. 221–54. [DOI]

31.

Cabib E, Sburlati A. Enzymatic determination of chitin. Methods Enzymol. 1988;161;457–9. [DOI]

32.

Brugnerotto J, Lizardi J, Goycoolea FM, Argüelles-Monal W, Desbrières J, Rinaudo M. An infrared investigation in relation with chitin and chitosan characterization. Polymer. 2001;42:3569–80. [DOI]

33.

Garnjanagoonchorn W, Wongekalak L, Engkagul A. Determination of chondroitin sulfate from different sources of cartilage. Chem Eng Process: Process Intensif. 2007;46:465–71. [DOI]

34.

de Oliveira SA, da Silva BC, Riegel-Vidotti IC, Urbano A, de Sousa Faria-Tischer PC, Tischer CA. Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb. Int J Biol Macromol. 2017;97:642–53. [DOI] [PubMed]