From:  Essential molecular biology methods in biomaterials research: a guide for emerging investigators

 Typical concentrations of the reagents are added to the reaction mixture

ComponentConcentrationVolume
Buffer (MgCl2)10×5 μL
dNTPs10 mM1 μL
Mg2+1.5 mM-
Each primer20–50 pmol-
Template DNA1–1,000 ng0.5 μL
DNA polymerase0.5–2.5 units-
Water-Complete 50 μL