Quantitative MTT test results of new formulation (L929 fibroblast cells)
Test material
Degree
Reaction
Situations of cultures
Negative control
0
Ø
Discrete intra-cytoplasmic granules, no cell destruction, no reduction in cell proliferation
Positive control
4
Strong
All or almost all of the cell layers were destroyed
Sample (1: 1)
0
Ø
Discrete intra-cytoplasmic granules, no cell destruction, no reduction in cell proliferation
Sample (1: 2)
0
Ø
Discrete intra-cytoplasmic granules, no cell destruction, no reduction in cell proliferation
Sample (1: 4)
0
Ø
Discrete intra-cytoplasmic granules, no cell destruction, no reduction in cell proliferation
Sample (1: 8)
0
Ø
Discrete intra-cytoplasmic granules, no cell destruction, no reduction in cell proliferation
Sample (1: 16)
0
Ø
Discrete intra-cytoplasmic granules, no cell destruction, no reduction in cell proliferation
Sample (1: 32)
0
Ø
Discrete intra-cytoplasmic granules, no cell destruction, no reduction in cell proliferation
Sample (1: 64)
0
Ø
Discrete intra-cytoplasmic granules, no cell destruction, no reduction in cell proliferation
Ø stands for no reaction seen, 0 stands for the reaction is not detectable and degradable
Declarations
Acknowledgments
The authors thank Mrs. Belgin Berker Budak for her support for this research. We also thank Mrs. Begüm Buğdaycı Açıkkol, Director of Kobay Exper. Animals Inc., Turkey.
Author contributions
GGB conducted this study and wrote this paper with SÖ. MB edited this manuscript. GGB did the compound formulation and HPLC analysis. TŞ performed LC–MS–MS analysis. BM applied QTOF analysis. ŞSM did total phenolic and flavonoid content determination. CÇÇ proceeded In vitro cytotoxicity and genotoxicity tests: In vivo acute/sub-acute oral toxicity and LD50 studies. OT performed In vivo acute/sub-acute oral toxicity and LD50 studies. MB assessed the tissue samples microscopically. SÖ made Statistical analysis. All authors have read and agreed to the published version of the manuscript.
Conflicts of interest
The authors declare no conflicts of interest.
Ethical approval
Institutional Review Board statement: the animal experiments were approved by the regional Ethical Committee for animal research. The licensing ethical committee approved the experiments, including any relevant details before the animal experiments (27.11.2020/514). The authors confirm that all experiments were performed in accordance with relevant international guidelines and regulations. The authors also complied with the ARRIVE guidelines. All manipulations were made to minimize animal suffering and reduce the number of animals used.
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