The schematic workflow of a comprehensive study on constructing and evaluating a risk score model based on M1 macrophage-related genes for calculating prognostic risk in NSCLC patients. Step 1. Four bulk RNA-seq and one scRNA-seq datasets were collected for this study. Step 2. Two M1-related gene lists were identified separately using WGCNA and maker gene screening algorithms. Step 3. The candidate M1-related factor genes were defined as the overlap between module genes and marker genes in the M1 sub-population. These factor genes were then filtered using LASSO regression analysis. Step 4. A risk score model was constructed using Cox regression analysis and validated by protein and copy number variation (CNV) levels