Inotropic crosslinking of chitosan with lignin (crosslinker)
Support cell adhesion (confirmed by HR SEM and fluorescence microscopy); porosity of hydrogel was found to be 72.53%; no cytotoxicity at 50 µg/mL concentration
HK: Conceptualization, Visualization, Data curation, Writing—review & editing, Writing—original draft. Disha: Data curation, Writing—review & editing. KS: Data curation, Writing—review & editing. OS: Project administration, Supervision, Writing—review & editing. BS: Conceptualization, Investigation, Project administration, Supervision, Writing—review & editing. All authors read and approved the submitted version.
Conflicts of interest
The authors declare that they have no conflicts of interest.
Ethical approval
Not applicable.
Consent to participate
Not applicable.
Consent to publication
Not applicable.
Availability of data and materials
Not applicable.
Funding
The authors would like to thank the Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala and Bioinformatics center (BIC) sponsored by Department of Biotechnology for providing a computational facility under the BIC (Bt/PR39876/Btis/137/7/2021), New Delhi, India. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.
References
Ullah F, Othman MB, Javed F, Ahmad Z, Md Akil H. Classification, processing and application of hydrogels: A review.Mater Sci Eng: C. 2015;57:414–33. [DOI] [PubMed]
Meng Y, Li C, Liu X, Lu J, Cheng Y, Xiao LP, et al. Preparation of magnetic hydrogel microspheres of lignin derivate for application in water.Sci Total Environ. 2019;685:847–55. [DOI] [PubMed]
Chamkouri H. A Review of Hydrogels, Their Properties and Applications in Medicine.Am J Biomed Sci Res. 2021;11:485–93. [DOI]
Mondal AK, Uddin MT, Sujan SMA, Tang Z, Alemu D, Begum HA, et al. Preparation of lignin-based hydrogels, their properties and applications.Int J Biol Macromol. 2023;245:125580. [DOI] [PubMed]
Xue B, Wen J, Sun R. Ethanol organosolv lignin as a reactive filler for acrylamide‐based hydrogels.J Appl Polym Sci. 2015;132:e132. [DOI]
Abdullah T, İlyasoğlu G, Memić A. Designing Lignin-Based Biomaterials as Carriers of Bioactive Molecules.Pharmaceutics. 2023;15:1114. [DOI] [PubMed] [PMC]
Khadem E, Ghafarzadeh M, Kharaziha M, Sun F, Zhang X. Lignin derivatives-based hydrogels for biomedical applications.Int J Biol Macromol. 2024;261:129877. [DOI] [PubMed]
Mukheja Y, Kaur J, Pathania K, Sah SP, Salunke DB, Sangamwar AT, et al. Recent advances in pharmaceutical and biotechnological applications of lignin-based materials.Int J Biol Macromol. 2023;241:124601. [DOI] [PubMed]
Khan S, Maryam L, Gulzar A, Mansoor MA, Iqbal M. Review: smart and active hydrogels in biotechnology—synthetic techniques and applications.J Mater Sci. 2024;59:16449–71. [DOI]
Zheng Y, Moreno A, Zhang Y, Sipponen MH, Dai L. Harnessing chemical functionality of lignin towards stimuli-responsive materials.Trends Chem. 2024;6:62–78. [DOI]
Karagoz P, Khiawjan S, Marques MPC, Santzouk S, Bugg TDH, Lye GJ. Pharmaceutical applications of lignin-derived chemicals and lignin-based materials: linking lignin source and processing with clinical indication.Biomass Convers Biorefinery. 2023;14:26553–74. [DOI] [PubMed] [PMC]
Behling R, Valange S, Chatel G. Heterogeneous catalytic oxidation for lignin valorization into valuable chemicals: what results? What limitations? What trends?Green Chem. 2016;18:1839–54. [DOI]
Akpan EI. Chemistry and Structure of Lignin.Sustain Lignin Carbon Fibers: Princ Tech Appl. 2019:1–50. [DOI]
Pandit S, Sharma P, Prakash A, Lal B, Bhuyan R, Ahmad I, et al. A comprehensive review on technical lignin, lignin hydrogels, properties, preparation, applications & challenges in lab to market transition.Ind Crops Prod. 2024;211:118262. [DOI]
Kaur R, Sharma R, Chahal GK. Synthesis of lignin-based hydrogels and their applications in agriculture: A review.Chem Pap. 2021;75:4465–78. [DOI]
Gujjala LKS, Kim J, Won W. Technical lignin to hydrogels: An Eclectic review on suitability, synthesis, applications, challenges and future prospects.J Clean Prod. 2022;363:132585. [DOI]
Gigli M, Crestini C. Fractionation of industrial lignins: opportunities and challenges.Green Chem. 2020;22:4722–46. [DOI]
Rawat K, Kaur R, Pujari AK, Gogde K, Mohne D, Kirar S, et al. Lignin-based biopharmaceuticals from nature to medicine: current trends, future prospects and emerging challenges.RSC Appl Interfaces. 2026. [DOI]
Kim TH, Park SH, Lee S, Bharadwaj AS, Lee YS, Yoo CG, et al. A Review of Biomass-Derived UV-Shielding Materials for Bio-Composites.Energies. 2023;16:2231. [DOI]
Wu Y, Wang H, Yuan L, Zhang Q, Liu Y, Shao C, et al. Lignin-Based Functional Hydrogels: An Eco-friendly Bulk Material.ACS Sustain Chem Eng. 2024;12:17952–76. [DOI]
Wang Q, Guo J, Lu X, Ma X, Cao S, Pan X, et al. Wearable lignin-based hydrogel electronics: A mini-review.Int J Biol Macromol. 2021;181:45–50. [DOI] [PubMed]
Liu R, Dai L, Xu C, Wang K, Zheng C, Si C. Lignin‐Based Micro‐ and Nanomaterials and their Composites in Biomedical Applications.ChemSusChem. 2020;13:4266–83. [DOI] [PubMed]
Quraishi S, Martins M, Barros AA, Gurikov P, Raman S, Smirnova I, et al. Novel non-cytotoxic alginate–lignin hybrid aerogels as scaffolds for tissue engineering.J Supercrit Fluids. 2015;105:1–8. [DOI]
Zhu W, Lu J, Dai L. Multifunctional pH‐Responsive Sprayable Hydrogel Based on Chitosan and Lignin‐Based Nanoparticles.Part Part Syst Charact. 2018;35:1800145. [DOI]
Liu C, Li Y, Zhuang J, Xiang Z, Jiang W, He S, et al. Conductive Hydrogels Based on Industrial Lignin: Opportunities and Challenges.Polymers. 2022;14:3739. [DOI] [PubMed] [PMC]
de Gonzalo G, Colpa DI, Habib MH, Fraaije MW. Bacterial enzymes involved in lignin degradation.J Biotechnol. 2016;236:110–9. [DOI] [PubMed]
Ravishankar K, Venkatesan M, Desingh RP, Mahalingam A, Sadhasivam B, Subramaniyam R, et al. Biocompatible hydrogels of chitosan-alkali lignin for potential wound healing applications.Mater Sci Eng: C. 2019;102:447–57. [DOI] [PubMed]
Li M, Jiang X, Wang D, Xu Z, Yang M. In situ reduction of silver nanoparticles in the lignin based hydrogel for enhanced antibacterial application.Colloids Surf B: Biointerfaces. 2019;177:370–6. [DOI] [PubMed]
Belgodere JA, Zamin SA, Kalinoski RM, Astete CE, Penrod JC, Hamel KM, et al. Modulating Mechanical Properties of Collagen–Lignin Composites.ACS Appl Bio Mater. 2019;2:3562–72. [DOI] [PubMed]
Wang X, Li X, Yadav C, Lan W, Wang L, Uraki Y, et al. Enhancing the mechanical performance of lignin based hydrogel via lignin acetylation.Ind Crops Prod. 2023;199:116780. [DOI]
Mondal AK, Xu D, Wu S, Zou Q, Lin W, Huang F, et al. Lignin-containing hydrogels with anti-freezing, excellent water retention and super-flexibility for sensor and supercapacitor applications.Int J Biol Macromol. 2022;214:77–90. [DOI] [PubMed]
Elsayed MM. Hydrogel Preparation Technologies: Relevance Kinetics, Thermodynamics and Scaling up Aspects.J Polym Environ. 2019;27:871–91. [DOI]
Ma Y, Sun Y, Fu Y, Fang G, Yan X, Guo Z. Swelling behaviors of porous lignin based poly (acrylic acid).Chemosphere. 2016;163:610–9. [DOI] [PubMed]
Lv S, Liang S, Zuo J, Zhang S, Wang J, Wei D. Lignin-based anti-UV functional materials: recent advances in preparation and application.Iran Polym J. 2023;32:1477–97. [DOI]
Li Y, Zhao S, Hu D, Ragauskas AJ, Cao D, Liu W, et al. Role Evaluation of Active Groups in Lignin on UV-Shielding Performance.ACS Sustain Chem Eng. 2022;10:11856–66. [DOI]
Raghuwanshi VS, Garnier G. Characterisation of hydrogels: Linking the nano to the microscale.Adv Colloid Interface Sci. 2019;274:102044. [DOI] [PubMed]
Zerpa A, Pakzad L, Fatehi P. Hardwood Kraft Lignin-Based Hydrogels: Production and Performance.ACS Omega. 2018;3:8233–42. [DOI] [PubMed] [PMC]
Kai D, Ren W, Tian L, Chee PL, Liu Y, Ramakrishna S, et al. Engineering Poly(lactide)–Lignin Nanofibers with Antioxidant Activity for Biomedical Application.ACS Sustain Chem Eng. 2016;4:5268–76. [DOI]
Wang J, Brugnoli B, Foglietta F, Andreana I, Longo G, Dinarelli S, et al. Tuning stiffness of hyaluronan-cholesterol nanogels by mussel-inspired dopamine-Fe3+ coordination: Preparation and properties evaluation.Int J Biol Macromol. 2024;280:135553. [DOI]
Wang Y, Liu S, Wang Q, Ji X, An X, Liu H, et al. Nanolignin filled conductive hydrogel with improved mechanical, anti-freezing, UV-shielding and transparent properties for strain sensing application.Int J Biol Macromol. 2022;205:442–51. [DOI] [PubMed]
Kim SJ, Park SJ, Kim SI. Swelling behavior of interpenetrating polymer network hydrogels composed of poly(vinyl alcohol) and chitosan.React Funct Polym. 2003;55:53–9. [DOI]
Sharma S, Kumar R, Kumari P, Kharwar RN, Yadav AK, Saripella S. Mechanically magnified chitosan-based hydrogel as tissue adhesive and antimicrobial candidate.Int J Biol Macromol. 2019;125:109–15. [DOI] [PubMed]
Mahamoud MM, Ketema TM, Kuwahara Y, Takafuji M. Enhancement of Mechanical Properties of Benign Polyvinyl Alcohol/Agar Hydrogel by Crosslinking Tannic Acid and Applying Multiple Freeze/Thaw Cycles.Gels. 2024;10:527. [DOI] [PubMed] [PMC]
Lin J, Chen M, Zhao W, Zhang S, Liu J, Zhou Y, et al. Lignin-Mediated Dual Conductive Hydrogels with High Conductivity, Antibacterial Activity and Biocompatibility for Chronic Wound Repair.Gels. 2025;11:283. [DOI] [PubMed] [PMC]
Zhu J. Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering.Biomaterials. 2010;31:4639–56. [DOI] [PubMed] [PMC]
Zustiak SP, Leach JB. Characterization of protein release from hydrolytically degradable poly(ethylene glycol) hydrogels.Biotechnol Bioeng. 2010;108:197–206. [DOI] [PubMed] [PMC]
Xia J, Liu Z, Chen Y, Wang Z, Cao Y. Fabrication of thermo-sensitive lignocellulose hydrogels with switchable hydrophilicity and hydrophobicity through an SIPN strategy.RSC Adv. 2019;9:29600–8. [DOI] [PubMed] [PMC]
Oveissi F, Naficy S, Le TYL, Fletcher DF, Dehghani F. Tough and Processable Hydrogels Based on Lignin and Hydrophilic Polyurethane.ACS Appl Bio Mater. 2018;1:2073–81. [DOI] [PubMed]
Hachimi Alaoui C, Réthoré G, Weiss P, Fatimi A. Sustainable Biomass Lignin-Based Hydrogels: A Review on Properties, Formulation, and Biomedical Applications.Int J Mol Sci. 2023;24:13493. [DOI] [PubMed] [PMC]
Yu C, Wang F, Zhang C, Fu S, Lucia LA. The synthesis and absorption dynamics of a lignin-based hydrogel for remediation of cationic dye-contaminated effluent.React Funct Polym. 2016;106:137–42. [DOI]
Jin Z, Yu Y, Shao S, Ye J, Lin L, Iiyama K. Lignin as a cross-linker of acrylic acid-grafted carboxymethyl lignocellulose.J Wood Sci. 2010;56:470–6. [DOI]
Munguía-Quintero MF, Vega-Hernández MÁ, Rosas-Aburto A, Hernández-Luna MG, López-Ramírez S, Barragán-Aroche JF, et al. An Early Study on the Synthesis of Lignin-Graft-(Net-Poly(acrylamide-co-N,N′methylenebisacrylamide)), Characterization of the Produced Copolymer, and Evaluation of Its Performance as Adsorbent for Lead Removal from Wastewater Purposes.Processes. 2023;11:2309. [DOI]
Passauer L. Highly Swellable Lignin Hydrogels: Novel Materials with Interesting Properties.ACS Symp Ser. 2012:211–28. [DOI]
Wang X, Zhou Z, Guo X, He Q, Hao C, Ge C. Ultrasonic-assisted synthesis of sodium lignosulfonate-grafted poly(acrylic acid-co-poly(vinyl pyrrolidone)) hydrogel for drug delivery.RSC Adv. 2016;6:35550–8. [DOI]
Rajan K, Mann JK, English E, Harper DP, Carrier DJ, Rials TG, et al. Sustainable Hydrogels Based on Lignin-Methacrylate Copolymers with Enhanced Water Retention and Tunable Material Properties.Biomacromolecules. 2018;19:2665–72. [DOI] [PubMed]
Gupta C, Washburn NR. Polymer-Grafted Lignin Surfactants Prepared via Reversible Addition–Fragmentation Chain-Transfer Polymerization.Langmuir. 2014;30:9303–12. [DOI] [PubMed]
Liu H, Chung H. Lignin‐based polymers via graft copolymerization.J Polym Sci A: Polym Chem. 2017;55:3515–28. [DOI]
Liu Z, Lu X, Xie J, Feng B, Han Q. Synthesis of a novel tunable lignin-based star copolymer and its flocculation performance in the treatment of kaolin suspension.Sep Purif Technol. 2019;210:355–63. [DOI]
Liu X, Yin H, Zhang Z, Diao B, Li J. Functionalization of lignin through ATRP grafting of poly(2-dimethylaminoethyl methacrylate) for gene delivery.Colloids Surf B: Biointerfaces. 2015;125:230–7. [DOI] [PubMed]
Xu Y, Li N, Wang G, Wang C, Chu F. Synthesis of Lignin-Based MMA-co-BA Hybrid Resins from Cornstalk Residue via RAFT Miniemulsion Polymerization and Their Characteristics.Polymers. 2021;13:968. [DOI] [PubMed] [PMC]
Wang Z, Wei H, Huang Y, Wei Y, Chen J. Naturally sourced hydrogels: emerging fundamental materials for next-generation healthcare sensing.Chem Soc Rev. 2023;52:2992–3034. [DOI] [PubMed]
Li YE. Sustainable Biomass Materials for Biomedical Applications.ACS Biomater Sci Eng. 2019;5:2079–92. [DOI] [PubMed]
Karunakaran C, Rajkumar R, Bhargava K. Introduction to Biosensors.Biosens Bioelectron. 2015:1–68. [DOI]
Ho JCH, Pawar SV, Hallam SJ, Yadav VG. An Improved Whole-Cell Biosensor for the Discovery of Lignin-Transforming Enzymes in Functional Metagenomic Screens.ACS Synth Biol. 2017;7:392–8. [DOI] [PubMed]
Wang Q, Pan X, Lin C, Lin D, Ni Y, Chen L, et al. Biocompatible, self-wrinkled, antifreezing and stretchable hydrogel-based wearable sensor with PEDOT:sulfonated lignin as conductive materials.Chem Eng J. 2019;370:1039–47. [DOI]
Wang Q, Pan X, Lin C, Ma X, Cao S, Ni Y. Ultrafast gelling using sulfonated lignin-Fe3+ chelates to produce dynamic crosslinked hydrogel/coating with charming stretchable, conductive, self-healing, and ultraviolet-blocking properties.Chem Eng J. 2020;396:125341. [DOI]
Zhang Y, Mao J, Jiang W, Zhang S, Tong L, Mao J, et al. Lignin sulfonate induced ultrafast polymerization of double network hydrogels with anti-freezing, high strength and conductivity and their sensing applications at extremely cold conditions.Compos B: Eng. 2021;217:108879. [DOI]
Wang Q, Pan X, Guo J, Huang L, Chen L, Ma X, et al. Lignin and cellulose derivatives-induced hydrogel with asymmetrical adhesion, strength, and electriferous properties for wearable bioelectrodes and self-powered sensors.Chem Eng J. 2021;414:128903. [DOI]
Hao Y, Wang C, Jiang W, Yoo CG, Ji X, Yang G, et al. Lignin-silver triggered multifunctional conductive hydrogels for skinlike sensor applications.Int J Biol Macromol. 2022;221:1282–93. [DOI] [PubMed]
Chen C, Zheng N, Wu W, Tang M, Feng W, Zhang W, et al. Self-Adhesive and Conductive Dual-Network Polyacrylamide Hydrogels Reinforced by Aminated Lignin, Dopamine, and Biomass Carbon Aerogel for Ultrasensitive Pressure Sensor.ACS Appl Mater Interfaces. 2022;14:54127–40. [DOI] [PubMed]
Wang Q, Zhang H, Pan X, Ma X, Cao S, Ni Y. Adhesive, Transparent Tannic Acid@ Sulfonated Lignin-PAM Ionic Conductive Hydrogel Electrode with Anti-UV, Antibacterial and Mild Antioxidant Function.Materials. 2019;12:4135. [DOI] [PubMed] [PMC]
Li X, Meng Y, Cheng Z, Li B. Research Progress and Prospect of Stimuli-Responsive Lignin Functional Materials.Polymers. 2023;15:3372. [DOI] [PubMed] [PMC]
Cai J, Zhang X, Liu W, Huang J, Qiu X. Synthesis of highly conductive hydrogel with high strength and super toughness.Polymer. 2020;202:122643. [DOI]
Zhao H, Hao S, Fu Q, Zhang X, Meng L, Xu F, et al. Ultrafast Fabrication of Lignin-Encapsulated Silica Nanoparticles Reinforced Conductive Hydrogels with High Elasticity and Self-Adhesion for Strain Sensors.Chem Mater. 2022;34:5258–72. [DOI]
Chen J, Li B, Ma X, Zhou S, Gu Q, Bian H, et al. Modified lignin‐induced composite hydrogels with good mechanical properties, adhesion, and UV resistance for strain sensors.J Appl Polym Sci. 2023;140:54643. [DOI]
Ren K, Shi Y, Wen C, Kang X, Tian Y, Guan Y, et al. Lignin-Based Conductive Hydrogels with Plasticity, Recyclability, and Self-Adhesion as Flexible Strain Sensors for Human Motion Monitoring.ACS Appl Polym Mater. 2024;6:5297–307. [DOI]
He Y, Sun S, Zhang X, Xu Y, Zhang C, Shao C, et al. Self-Adhesive, Anti-Freezing Multifunctional Zwitterionic Hydrogels with Lignin-Promoted Rapid Gelation for Flexible Strain Sensors.ACS Sustain Chem Eng. 2024;12:11809–20. [DOI]
Luo J, Hu Y, Luo S, Wang X, Chen S, Zhang M, et al. Strong and Multifunctional Lignin/Liquid Metal Hydrogel Composite as Flexible Strain Sensors.ACS Sustain Chem Eng. 2024;12:7105–14. [DOI]
Huang J, Yang Y, Chao L, Liu W, Li H, Zhao L, et al. A tough lignin/nanocellulose based hydrogels strain sensor with ultrafast gelling process.Cellulose. 2025;32:2289–301. [DOI]
Du T, Su X, Zhu Y, Zhao G, Zhang M, Li C, et al. Efficient Fabrication of Highly Elastic, Self-Adhesive MXene-Doped Lignin-Based Conductive Hydrogels for Flexible Strain Sensing Applications.ACS Appl Electron Mater. 2025;7:2862–72. [DOI]
Han X, Lv Z, Ran F, Dai L, Li C, Si C. Green and stable piezoresistive pressure sensor based on lignin-silver hybrid nanoparticles/polyvinyl alcohol hydrogel.Int J Biol Macromol. 2021;176:78–86. [DOI] [PubMed]
Ma Y, Liu N, Li L, Hu X, Zou Z, Wang J, et al. A highly flexible and sensitive piezoresistive sensor based on MXene with greatly changed interlayer distances.Nat Commun. 2017;8:1207. [DOI] [PubMed] [PMC]
Wang Q, Lan J, Hua Z, Ma X, Chen L, Pan X, et al. An oriented Fe3+-regulated lignin-based hydrogel with desired softness, conductivity, stretchability, and asymmetric adhesiveness towards anti-interference pressure sensors.Int J Biol Macromol. 2021;184:282–8. [DOI] [PubMed]
Miao Y, Tang Z, Zhang Q, Reheman A, Xiao H, Zhang M, et al. Biocompatible Lignin-Containing Hydrogels with Self-Adhesion, Conductivity, UV Shielding, and Antioxidant Activity as Wearable Sensors.ACS Appl Polym Mater. 2022;4:1448–56. [DOI]
Jin Z, Gong H, Chen B, Jiang Y, Su Y, Zhou J, et al. Novel functional hydrogels based on ligninsilver nanoparticles with adhesion, antimicrobial, antioxidant and anti-freezing properties for wound dressings and pressure strain sensors.Int J Biol Macromol. 2025;291:138853. [DOI] [PubMed]
Kesharwani P, Bisht A, Alexander A, Dave V, Sharma S. Biomedical applications of hydrogels in drug delivery system: An update.J Drug Deliv Sci Technol. 2021;66:102914. [DOI]
Yang L, Yuan QY, Lou CW, Li TT, Lin JH. Modified Nanocellulose Hydrogels and Applications in Sensing Fields.Gels. 2025;11:140. [DOI] [PubMed] [PMC]
Slaughter BV, Khurshid SS, Fisher OZ, Khademhosseini A, Peppas NA. Hydrogels in Regenerative Medicine.Adv Mater. 2009;21:3307–29. [DOI] [PubMed] [PMC]
Moreno A, Sipponen MH. Lignin-based smart materials: a roadmap to processing and synthesis for current and future applications.Mater Horiz. 2020;7:2237–57. [DOI]
Li J, Mooney DJ. Designing hydrogels for controlled drug delivery.Nat Rev Mater. 2016;1:e1. [DOI] [PubMed] [PMC]
Preet J, Pathania K, Kaur J, Singh R, Salunke DB, Pawar SV. A lignin-based biocomposite hydrogel for antimicrobial and wound healing applications.Mater Adv. 2024;5:9445–57. [DOI]
Morales A, Labidi J, Gullón P. Influence of lignin modifications on physically crosslinked lignin hydrogels for drug delivery applications.Sustain Mater Technol. 2022;33:e00474. [DOI]
Balk M, Sofia P, Neffe AT, Tirelli N. Lignin, the Lignification Process, and Advanced, Lignin-Based Materials.Int J Mol Sci. 2023;24:11668. [DOI] [PubMed] [PMC]
Pajer N, Cestari C, Argyropoulos DS, Crestini C. From lignin self assembly to nanoparticles nucleation and growth: A critical perspective.npj Mater Sustain. 2024;2:31. [DOI]
Zhou Y, Han Y, Li G, Chu F. Effects of Lignin-Based Hollow Nanoparticle Structure on the Loading and Release Behavior of Doxorubicin.Materials. 2019;12:1694. [DOI] [PubMed] [PMC]
Oliveira SC, Pereira FM, Ferraz A, Silva FT, Gonçalves AR. Mathematical Modeling of Controlled-Release Systems of Herbicides Using Lignins as Matrices.Appl Biochem Biotechnol. 2000;84-86:595–615. [DOI] [PubMed]
Tian Z, Li G, Chen X, Li C, Liu R, Yue X, et al. Innovative lignin-based MOFs and COFs for biomedicine, energy storage, and environmental remediation.Adv Compos Hybrid Mater. 2025;8:114. [DOI]
Farhat W, Venditti R, Mignard N, Taha M, Becquart F, Ayoub A. Polysaccharides and lignin based hydrogels with potential pharmaceutical use as a drug delivery system produced by a reactive extrusion process.Int J Biol Macromol. 2017;104:564–75. [DOI] [PubMed]
Culebras M, Barrett A, Pishnamazi M, Walker GM, Collins MN. Wood-Derived Hydrogels as a Platform for Drug-Release Systems.ACS Sustain Chem Eng. 2021;9:2515–22. [DOI] [PubMed] [PMC]
Anghel N, Melinte V, Spiridon I, Pertea M. Antioxidant, Antimicrobial, and Kinetic Studies of Β-Cyclodextrin Crosslinked with Lignin for Drug Delivery.Pharmaceutics. 2022;14:2260. [DOI] [PubMed] [PMC]
Chiani E, Beaucamp A, Hamzeh Y, Azadfallah M, Thanusha AV, Collins MN. Synthesis and characterization of gelatin/lignin hydrogels as quick release drug carriers for Ribavirin.Int J Biol Macromol. 2023;224:1196–205. [DOI] [PubMed]
Cheng G, Wang S, Li W, Jiang Y, Liu X, Huang Q. A lignocellulose nanofibril-poly(vinyl alcohol) hydrogel with controlled drug delivery for wound healing.Ind Crops Prod. 2024;220:119234. [DOI]
Zarei Z, Kharaziha M, Karimzadeh F, Khadem E. Synthesis and biological applications of nanocomposite hydrogels based on the methacrylation of hydroxypropyl methylcellulose and lignin loaded with alpha-pinene.Carbohydr Polym. 2024;346:122642. [DOI] [PubMed]
Nirbhavane P, Kale SS, Kumar S, Athare T, Magar AG, Chalikwar SS, et al. Cotton stalk derived lignin-based hydrogel and its therapeutic utility in diabetic retinopathy.Biochem Biophys Res Commun. 2025;775:152123. [DOI] [PubMed]
Singh V, Dhukia S, Khullar L, Sihag S, Kaur R, Harjai K, et al. Synthesis and Characterization of Biomass Derived Modified Lignin Based PVA Hydrogel as Drug Delivery System for Ciprofloxacin.ChemistrySelect. 2025;10:202404597. [DOI]
Zhang W, Liu L, Cheng H, Zhu J, Li X, Ye S, et al. Hydrogel-based dressings designed to facilitate wound healing.Mater Adv. 2024;5:1364–94. [DOI]
Zhang L, Liu M, Zhang Y, Pei R. Recent Progress of Highly Adhesive Hydrogels as Wound Dressings.Biomacromolecules. 2020;21:3966–83. [DOI] [PubMed]
Gounden V, Singh M. Hydrogels and Wound Healing: Current and Future Prospects.Gels. 2024;10:43. [DOI] [PubMed] [PMC]
Zheng L, Lu G, Pei W, Yan W, Li Y, Zhang L, et al. Understanding the relationship between the structural properties of lignin and their biological activities.Int J Biol Macromol. 2021;190:291–300. [DOI] [PubMed]
Dizhbite T, Telysheva G, Jurkjane V, Viesturs U. Characterization of the radical scavenging activity of lignins??natural antioxidants.Bioresour Technol. 2004;95:309–17. [DOI] [PubMed]
Ciolacu DE, Nicu R, Suflet DM, Rusu D, Darie-Nita RN, Simionescu N, et al. Multifunctional Hydrogels Based on Cellulose and Modified Lignin for Advanced Wounds Management.Pharmaceutics. 2023;15:2588. [DOI] [PubMed] [PMC]
Lourençon TV, de Lima GG, Ribeiro CSP, Hansel FA, Maciel GM, da Silva K, et al. Antioxidant, antibacterial and antitumoural activities of kraft lignin from hardwood fractionated by acid precipitation.Int J Biol Macromol. 2021;166:1535–42. [DOI] [PubMed]
Gordobil O, Herrera R, Yahyaoui M, İlk S, Kaya M, Labidi J. Potential use of kraft and organosolv lignins as a natural additive for healthcare products.RSC Adv. 2018;8:24525–33. [DOI] [PubMed] [PMC]
Reyes DC, Ma Z, Romero JJ. The Antimicrobial Properties of Technical Lignins and Their Derivatives—A Review.Polymers. 2024;16:2181. [DOI] [PubMed] [PMC]
Mahata D, Jana M, Jana A, Mukherjee A, Mondal N, Saha T, et al. Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation.Sci Rep. 2017;7:46412. [DOI] [PubMed] [PMC]
Larrañeta E, Imízcoz M, Toh JX, Irwin NJ, Ripolin A, Perminova A, et al. Synthesis and Characterization of Lignin Hydrogels for Potential Applications as Drug Eluting Antimicrobial Coatings for Medical Materials.ACS Sustain Chem Eng. 2018;6:9037–46. [DOI] [PubMed] [PMC]
Gan D, Xing W, Jiang L, Fang J, Zhao C, Ren F, et al. Plant-inspired adhesive and tough hydrogel based on Ag-Lignin nanoparticles-triggered dynamic redox catechol chemistry.Nat Commun. 2019;10:1487. [DOI] [PubMed] [PMC]
Xu C, Liu L, Renneckar S, Jiang F. Chemically and physically crosslinked lignin hydrogels with antifouling and antimicrobial properties.Ind Crops Prod. 2021;170:113759. [DOI]
Cao J, Zhao Y, Jin S, Li J, Wu P, Luo Z. Flexible Lignin-based hydrogels with Self-healing and adhesive ability driven by noncovalent interactions.Chem Eng J. 2022;429:132252. [DOI]
Wang B, Qiu D, Gu Y, Shan Z, Shi R, Luo J, et al. A lignin-based controlled/sustained release hydrogel by integrating mechanical strengthening and bioactivities of lignin.J Bioresour Bioprod. 2025;10:62–76. [DOI]
Antunes F, Mota IF, Fangueiro JF, Lopes G, Pintado M, Costa PS. From sugarcane to skin: Lignin as a multifunctional ingredient for cosmetic application.Int J Biol Macromol. 2023;234:123592. [DOI] [PubMed]
Sadeghifar H, Ragauskas A. Lignin as a UV Light Blocker—A Review.Polymers. 2020;12:1134. [DOI] [PubMed] [PMC]
Darmawan MA, Ramadhani NH, Hubeis NA, Ramadhan MYA, Sahlan M, Abd-Aziz S, et al. Natural sunscreen formulation with a high sun protection factor (SPF) from tengkawang butter and lignin.Ind Crops Prod. 2022;177:114466. [DOI]
Wang F, Nithianandam S, Pylypchuk I, Sipponen MH. Lignin gel emulsions for environmentally benign hair conditioning.Sci Adv. 2025;11:eadr8372. [DOI] [PubMed] [PMC]
Trinh TA, Nguyen TL, Kim J. Lignin-Based Antioxidant Hydrogel Patch for the Management of Atopic Dermatitis by Mitigating Oxidative Stress in the Skin.ACS Appl Mater Interfaces. 2024;16:33135–48. [DOI] [PubMed]
Chavooshi R, Ranjkesh MR, Hashemi B, Roshangar L. Cellulose and Lignin-Derived Scaffold and Their Biological Application in Tissue Engineering, Drug Delivery, and Wound Healing: A Review.Cell J. 2023;25:158–64. [DOI] [PubMed] [PMC]
Shorey R, Salaghi A, Fatehi P, Mekonnen TH. Valorization of lignin for advanced material applications: a review.RSC Sustain. 2024;2:804–31. [DOI]
Restu WK, Rusumayanti F, Septiyanti M, Muryanto, Aryana N, Mawarni RS, et al. Formulation and Characteristics of Sunscreen Cream based on Isolated Lignin from Oil Palm Empty Fruit Bunch (OPEFB).J Sains Materi Indones. 2025;26:122–30. [DOI]
Silva MN, Scopel E, Rezende CA. From black liquor to tinted sunscreens: Washing out Kraft lignin unpleasant odor and improving its properties by lignin nanoparticle preparation.Ind Crops Prod. 2024;218:118910. [DOI]
Sarengan N, Raja PB, Safian MT, Ibrahim MNM. A review on tailoring lignin derivatives for sunscreen formulations: Structural modification and antioxidant properties for enhanced UV protection.Int J Biol Macromol. 2025;318:144871. [DOI] [PubMed]
Dhandayuthapani B, Yoshida Y, Maekawa T, Kumar DS. Polymeric Scaffolds in Tissue Engineering Application: A Review.Int J Polym Sci. 2011;2011:290602. [DOI]
El-Sherbiny IM, Yacoub MH. Hydrogel scaffolds for tissue engineering: Progress and challenges.Glob Cardiol Sci Pract. 2013;2013:316–42. [DOI] [PubMed] [PMC]
Pei W, Deng J, Wang P, Wang X, Zheng L, Zhang Y, et al. Sustainable lignin and lignin-derived compounds as potential therapeutic agents for degenerative orthopaedic diseases: A systemic review.Int J Biol Macromol. 2022;212:547–60. [DOI] [PubMed]
Bakshi MI, Nazir S, Restu WK, Rajamanickam R, Selvasembian R, Hua LS, et al. Recent advances in lignin from forest residue for hydrogel application.Biomass Convers Biorefinery. 2024;15:11475–91. [DOI]
Chen Y, Zheng K, Niu L, Zhang Y, Liu Y, Wang C, et al. Highly mechanical properties nanocomposite hydrogels with biorenewable lignin nanoparticles.Int J Biol Macromol. 2019;128:414–20. [DOI] [PubMed]
Wang D, Jang J, Kim K, Kim J, Park CB. “Tree to Bone”: Lignin/Polycaprolactone Nanofibers for Hydroxyapatite Biomineralization.Biomacromolecules. 2019;20:2684–93. [DOI] [PubMed]
Zheng L, Yu P, Zhang Y, Wang P, Yan W, Guo B, et al. Evaluating the bio-application of biomacromolecule of lignin-carbohydrate complexes (LCC) from wheat straw in bone metabolism via ROS scavenging.Int J Biol Macromol. 2021;176:13–25. [DOI] [PubMed]
Abudula T, Colombani T, Alade T, Bencherif SA, Memić A. Injectable Lignin-co-Gelatin Cryogels with Antioxidant and Antibacterial Properties for Biomedical Applications.Biomacromolecules. 2021;22:4110–21. [DOI] [PubMed]
Sood N, Bhardwaj A, Mehta S, Mehta A. Stimuli-responsive hydrogels in drug delivery and tissue engineering.Drug Deliv. 2014;23:758–80. [DOI] [PubMed]
Masteikova R, Chalupova Z, Sklubalova Z. Stimuli-sensitive hydrogels in controlled and sustained drug delivery.Medicina. 2003;39:19–24.
Dai L, Ma M, Xu J, Si C, Wang X, Liu Z, et al. All-Lignin-Based Hydrogel with Fast pH-Stimuli Responsiveness for Mechanical Switching and Actuation.Chem Mater. 2020;32:4324–30. [DOI]
Li T, Wang S, Huang Y, Zhou H, Zhang L, Wang Z. Lignin-induced rapid-synthesis of deep eutectic solvent-based gel with high robustness and conductivity applied for flexible quasi-solid-state supercapacitors.Chem Eng J. 2023;472:144864. [DOI]
Li X, Zhang L, Liu Z, Wang R, Jiao T. Recent progress in hydrogels combined with phototherapy for bacterial infection: A review.Int J Biol Macromol. 2024;274:133375. [DOI] [PubMed]
Nanda D, Behera D, Pattnaik SS, Behera AK. Advances in natural polymer-based hydrogels: synthesis, applications, and future directions in biomedical and environmental fields.Discov Polym. 2025;2:6. [DOI]
Kumar AC, Erothu H. Synthetic polymer hydrogels. Biomedical applications of polymeric materials and composites. In: Francis R, Kumar DS, editors. Biomedical applications of polymeric materials and composites. Germany: John Wiley & Sons; 2016. pp. 141–62. [DOI]
Jin C, Song W, Liu T, Xin J, Hiscox WC, Zhang J, et al. Temperature and pH Responsive Hydrogels Using Methacrylated Lignosulfonate Cross-Linker: Synthesis, Characterization, and Properties.ACS Sustain Chem Eng. 2018;6:1763–71. [DOI]
Chen Q, Li S, Zhao W, Zhao C. A rapid-triggered approach towards antibacterial hydrogel wound dressing with synergic photothermal and sterilization profiles.Biomater Adv. 2022;138:212873. [DOI] [PubMed]
Chandna S, Paul S, Kaur R, Gogde K, Bhaumik J. Photodynamic Lignin Hydrogels: A Versatile Self-Healing Platform for Sustained Release of Photosensitizer Nanoconjugates.ACS Appl Polym Mater. 2022;4:8962–76. [DOI]
Chao Y, Yu S, Zhang H, Gong D, Li J, Wang F, et al. Architecting Lignin/Poly(vinyl alcohol) Hydrogel with Carbon Nanotubes for Photothermal Antibacterial Therapy.ACS Appl Bio Mater. 2023;6:1525–35. [DOI] [PubMed]
Tadesse MG, Lübben JF. Review on Hydrogel-Based Flexible Supercapacitors for Wearable Applications.Gels. 2023;9:106. [DOI] [PubMed] [PMC]
Luo P, Liu Q, Chen R, Shao H, Ma Y, Zhao Y. Recent progress in biocompatible miniature supercapacitors.Energy Mater. 2025;5:e5. [DOI]
Muralee Gopi CVV, Alzahmi S, Narayanaswamy V, Raghavendra KVG, Issa B, Obaidat IM. A review on electrode materials of supercapacitors used in wearable bioelectronics and implantable biomedical applications.Mater Horiz. 2025;12:4092–132. [DOI] [PubMed]
Yi Y, Zhuang J, Liu C, Lei L, He S, Hou Y. Emerging Lignin-Based Materials in Electrochemical Energy Systems.Energies. 2022;15:9450. [DOI]
Li F, Wang X, Sun R. A metal-free and flexible supercapacitor based on redox-active lignosulfonate functionalized graphene hydrogels.J Mater Chem A. 2017;5:20643–50. [DOI]
Park JH, Rana HH, Lee JY, Park HS. Renewable flexible supercapacitors based on all-lignin-based hydrogel electrolytes and nanofiber electrodes.J Mater Chem A. 2019;7:16962–8. [DOI]
Liu T, Ren X, Zhang J, Liu J, Ou R, Guo C, et al. Highly compressible lignin hydrogel electrolytes via double-crosslinked strategy for superior foldable supercapacitors.J Power Sources. 2020;449:227532. [DOI]
Cui L, Li Y, Jia M, Cheng C, Jin X. A Self-Assembled and Flexible Supercapacitor based on Redox-Active Lignin-Based Nitrogen-Doped Activated Carbon Functionalized Graphene Hydrogels.J Electrochem Soc. 2021;168:053504. [DOI]
