Ex/In vivo 4-functional contribution rate of ACs (A) alone or mixed with BPSDS or BSS via [(ACs – V) – (V – S)]/[(BPSDS or BSS – V) – (V – S)] calculating.
| Group/Treatment | Ex vivo detection of H2O2-treated RAEC cells | Extrapolated value/% difference | In vitro detection of acute forced swim | ||||||
|---|---|---|---|---|---|---|---|---|---|
| NO (μmol/L)Mean ± SDContribution | eNOS (μmol/L)Mean ± SDContribution | ROS (μmol/L)Mean ± SDContribution | VTIMean ± SDContribution | FSTMean ± SDContribution | GEMean ± SDContribution | IPMean ± SDContribution | Error range% | ||
| S | 22.30 ± 0.22 | 37.20 ± 1.19 | 2,433.60 ± 41.88 | — | 28.75 ± 5.08 | 82.60 ± 12.68 | 0.795 ± 0.053 | 0.793 ± 0.055 | — |
| — | — | — | — | — | — | — | — | — | |
| V | 11.00 ± 0.52 | 17.53 ± 1.36 | 20,432.47 ± 411.95 | — | 18.35 ± 1.63 | 179.63 ± 3.70 | 0.587 ± 0.106 | 0.601 ± 0.075 | — |
| — | — | — | — | — | — | — | — | — | |
| BPSDS or BSS | 20.76 ± 1.10 | 33.76 ± 2.26 | 7,652.73 ± 65.49 | — | 25.87 ± 5.85* | 103.70 ± 5.56* | 0.722 ± 0.073* | 0.721 ± 0.037* | — |
| — | — | — | — | — | — | — | — | — | |
| MH | 18.13 ± 0.23 | 24.00 ± 0.11 | 11,328.17 ± 174.61 | NO 92.1% | 21.20 ± 0.98 | 97.06 ± 25.92 | 0.882 ± 0.091 | 0.734 ± 0.052 | — |
| 92.1% | 72.81% | 88.06% | eNOS 72.81% | 73.94% | 103.84% | 146.65%# | 104.14% | — | |
| — | — | — | Difference | 1.13% | 11.74% | 54.55% | 12.04% | 1.13–12.04 | |
| FA | 16.96 ± 0.41 | 23.61 ± 0.71 | 15,090.6 ± 188.99 | NO 81.96% | 28.07 ± 0.52 | 120.37 ± 10.37 | 0.729 ± 0.104 | 0.882 ± 0.046 | — |
| 81.96% | 71.73% | 75.83% | — | 112.28% | 90.36% | 102.04% | 151.27%# | — | |
| — | — | — | Difference | 30.32% | 8.4% | 20.08% | 69.31% | 8.4–30.32 | |
| MH + FA | 20.96 ± 0.52 | 32.17 ± 0.70 | 7,683.34 ± 174.82 | NO 100.94% | 18.77 ± 19.71 | 95.77 ± 19.71 | 0.643 ± 0.014 | 0.650 ± 0.042 | — |
| 100.94% | 95.57% | 100.09% | eNOS 95.57% | 60.37% | 104.59% | 76.97% | 77.39% | — | |
| — | — | — | Difference | 35.2% | 3.65% | 18.6% | 18.18% | 3.65–35.2 | |
| 2 ACs | 19.67 ± 0.68 | 45.97 ± 0.61 | 2,721.30 ± 84.48 | NO 94.82% | 23.61 ± 1.57 | 123.92 ± 24.11 | — | — | — |
| 94.82% | 134.01% | 116.02% | — | 87.39% | 88.31% | — | — | — | |
| — | — | — | Difference | 7.43% | 6.51% | — | — | 6.51–7.43 | |
| 3 ACs | 16.29 ± 0.61 | 30.22 ± 1.84 | 2,230.70 ± 14.39 | eNOS 90.14% | — | — | 0.547 ± 0.096 | 0.717 ± 0.061 | — |
| 78.77% | 90.14% | 117.62% | — | — | 48.98% | 98.73% | — | ||
| — | — | — | Difference | — | — | 41.16% | 8.59% | 8.59–41.16 | |
| 10 ACs | 19.24 ± 1.39 | 29.52 ± 2.66 | 11,911.43 ± 70.90 | NO 97.78% | 25.22 ± 2.52 | 118.52 ± 14.81 | 0.904 ± 0.095 | 0.765 ± 0.080 | — |
| 97.78% | 88.19% | 86.16% | eNOS 88.19% | 96.37% | 91.43% | 153.06%# | 114.01% | — | |
| — | — | — | Difference | 1.41% | 6.35% | 55.28% | 16.23% | 1.41–16.23 | |
#: As strong prokinetic trait docking traditional therapy; 2 ACs: Saikosaponin A + hesperidin; 3 ACs: Saikosaponin A + hesperidin + tangeretin. V: Vehicle; RAECs: rat aortic endothelial cells; BSS: Bupleurum chinense Shugan-San; eNOS: endothelial nitric oxide; GE: gastric emptying; MH: meranzin hydrate; S: Sham; VTI: velocity-time integrals; FA: ferulic acid.