Concordance of BRAF mutational status established by RT-PCR and NGS methods

RT-PCR Total
BRAF mutated BRAF wild-type
NGS BRAF mutated 12 2 14
BRAF wild-type 0 15 15
Total 12 17 29

Kappa = 0.89, 95% confidence interval (CI) 0.68 ± 1.05. Concordance samples are in bold.