Table Info

Table 1.

Characteristics of TAM-resistant breast cancer cell lines derived from MCF-7

Parental cell line	TAM resistant derivative
Parental cell line	Cell line	Method of induction of TAM resistance
WT MCF-7 (oestrogen-dependent, TAM-sensitive)	TAMr 1	Chronic TAM exposure (12 months)
	TAMr 2	Chronic TAM exposure (21 months)
	MCF-7-5C	Long-term oestrogen deprivation
	MCF-7-WS8	Long-term oestrogen deprivation
MCF-7/LCC1 (oestrogen-independent, endocrine therapy responsive)	LCC2	Stepwise selection against TAM
	LCC9	Stepwise selection against fulvestrant

Declarations

Acknowledgments

We thank Werbena Hamilton-Burke, Loaie Maraqa and Mark Peter for providing clinical data on the breast tumours which populated the TMAs used in this study.

Author contributions

Conception and design: MAH, VS. Acquisition of data: AMS, BA, PYM, BMB, RH, PF, HB, MV, MC, VCJ, VS. Analysis and interpretation of data: AMS, BA, PYM, BMB, RH, PF, HB, MV, MC, VCJ, VS. Writing and/or revision of the manuscript: MV, MC, MAH, VS. All authors read and approved the manuscript before submission.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

Access to histopathology archives for TMA construction and immunohistochemistry was approved by the Leeds (East) Research Ethics (06/Q1206/180).

Consent to participate

The informed consent to participate in the study was obtained from all participants.

Consent to publication

The informed consent to publication was obtained from relevant participants.

Availability of data and materials

Part of the results described is based upon data generated by the TCGA Research Network (https://www.cancer.gov/about-nci/organization/ccg/research/structural-genomics/tcga), the online resources of KMplot (https://kmplot.com/analysis/) and OncoLnc (http://www.oncolnc.org/).

Funding

This work was supported in part by Cancer Research UK grant C13432/A9047; Yorkshire Cancer Research grant L314; the Robert Bosch Foundation, Stuttgart, Germany; the NIH; MD Anderson’s Cancer Center support grant CA016672; the George and Barbara Bush Foundation for Innovative Cancer Research; and the Dallas/Fort Worth Living Legend Chair of Cancer Research. The study sponsor(s) played no part in the study design; in the collection, analysis and interpretation of the data; in the writing of the report; and in the decision to submit the paper for publication.

Copyright

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