Exploration of Digestive Diseases

Table 1. The effect of dietary cholic acid in high-fat diet model on body parameters

Parameters	ND	HFD	HFDCA
Initial body weight (g)	19.23 ± 0.48	19.56 ± 0.66	19.56 ± 0.32
Final body weight (g)	24.87 ± 0.90^b	30.12 ± 1.59^a	26.37 ± 0.73^ab
eWAT weight (g)	0.63 ± 0.07^b	1.32 ± 0.16^a	0.89 ± 0.06^b
eWAT: body weight (%)	2.74 ± 0.24^b	4.94 ± 0.44^a	3.52 ± 0.21^b
Liver weight (g)	0.87 ± 0.03^b	0.86 ± 0.03^b	1.13 ± 0.02^a
Liver: body weight (%)	3.82 ± 0.06^b	3.29 ± 0.06^c	4.51 ± 0.12^a

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Mice were fed for 6 weeks. Treatment groups: normal diet (ND), high-fat diet with 60% fat (HFD), high-fat diet with 60% fat and 0.5% (w/w) cholic acid (HFDCA). All values are expressed as mean ± SEM (n = 8). Values marked with different letters (a, b, c) are significantly different (p < 0.05) in the Tukey-Kramer post hoc test. eWAT: epididymal white adipose tissue

Supplementary materials

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Declarations

Acknowledgments

The authors thank Dr. Adi Shpaizer for her part in the diet preparation and animals’ maintenance and Dr. Zohar Gavish at Gavish Research Services Ltd for performing the histological work.

Author contributions

DAH: Conceptualization, Methodology, Formal analysis, Investigation, Data curation, Writing—original draft, Writing—review & editing, Visualization. ER: Investigation, Formal analysis. HZ: Methodology, Formal analysis, Writing—review & editing. OT: Conceptualization, Methodology, Validation, Resources, Writing—original draft, Writing—review & editing, Supervision, Project administration, Funding acquisition. All authors have read and agreed to the published version of the manuscript.

Conflicts of interest

Prof. Oren Tirosh is an Associate Editor and Guest Editor of Exploration of Digestive Diseases. However, he was not involved in any aspect of the peer review or editorial decision-making process for this manuscript. The other authors declare no conflicts of interest.

Ethical approval

All animal procedures were performed following the guidelines of the Guide for the Care and Use of Laboratory Animals. The study was approved by the Animal Ethics Committee of the Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, approval number: AG-22-17063-3.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

Data will be available upon request.

Funding

This work is funded by internal funding from the Hebrew University of Jerusalem. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

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