Main human vimentin phosphorylation sites

GeneSitePutative kinaseDiscovered by/inhibitorEffectReference
VIMSer5/7/8/9/10PKCαCalyculin A (phosphatase)Cell polarity[63]
Ser26/51/66PAK1Biochemical assays, selumetinib, vemurafenibAbnormal assembly[113, 114]
Ser39AKT/PKBA-674563Proteolytic protection, slowed polymerization?[69]
Ser56AKT/PKB
ROCK
A-674563
siRNA
Filament disassembly[69]
[72]
Ser72ROCK1Targeted mutation, Y27632Increased cell migration[75, 76, 115]
Ser73AURKBBiochemical assaysMitosis?[114, 116]
Ser83PLK-1, CaMKIIBiochemical assays, KN-93 (CaMKII inhibitor)Filament disassembly and pathogen interaction[77, 117, 118]

CaMKII: calmodulin-dependent protein kinase II