From:  Response-adaptive breast cancer care in the grey zones: integrating ER and HER2 targeted PET, FDG PET/CT under immunotherapy, and ctDNA kinetics

 Succinct summary of the response-adaptive breast cancer care in the grey zones.

TopicCore valueMain clinical use caseKey limitationTake-home message
ER-targeted PET ([18F]FES PET/CT)Whole-body functional ER mapping (captures heterogeneity and receptor discordance)Suspected ER discordance, mixed endocrine response, biopsy not feasiblePhysiologic/background uptake in some organs; not a substitute for histology in all cases
Evidence status: supported by meta-analyses and growing clinical experience in selected ER-positive scenarios; not a universal replacement for biopsy.
Best used to answer a specific endocrine decision question (continue/switch endocrine strategy, target biopsy)
HER2-targeted PETWhole-body HER2 expression assessment beyond a single biopsySuspected HER2 conversion/heterogeneity in metastatic disease, especially when tissue is inaccessibleLimited availability, logistics, tracer timing, and standardisation
Evidence status: promising but mostly small, feasibility-based or specialised-centre evidence; clinical utility still requires validation.
Useful as a problem-solving biomarker to support anti-HER2 treatment selection or biopsy prioritisation
FDG PET/CT under immunotherapyWhole-body tumour burden + early metabolic response + potential detection of immune-related adverse eventsResponse assessment during immune checkpoint inhibitor treatment; equivocal progressionPseudoprogression/dissociated response can mislead early interpretation
Evidence status: Biologically plausible and clinically useful in selected cases, but immunotherapy-specific criteria require TNBC-specific validation.
Interpret with immunotherapy-aware criteria and confirmation strategy
FDG PET/CT + ctDNA kinetics (combined approach)Combines spatial lesion-level information (PET) with temporal systemic molecular dynamics (ctDNA)Early escalation/de-escalation decisions, discordant response patterns, oligoprogressionTiming/threshold harmonisation, assay variability, and risk of overtesting
Evidence status: conceptual and trial-ready; not yet validated for routine escalation/de-escalation.
Most powerful when embedded in a predefined action algorithm

[18F]FES: 16α-[18F]fluoro-17β-estradiol; ctDNA: circulating tumour DNA; ER: oestrogen receptor; TNBC: triple-negative breast cancer; HER2: human epidermal growth factor receptor 2; PET/CT: positron emission tomography/computed tomography.