From:  ESR1 mutations in ER-positive breast cancer: from endocrine resistance to ctDNA-guided therapeutic interception

 Clinical trials of oral SERDs and ctDNA-guided switching strategies in ESR1-mutant ER+/HER2advanced breast cancer.

Clinical trialAgentPopulationIntervention armComparator armMedian PFS (ESR1-mut)HR (95% CI)Key findingsReferences
Classical trials: testing at progression
EMERALDElacestrantPost-CDK4/6i progression; ER+/HER2– MBCElacestrantSOC ET (fulvestrant or AI)3.6 vs. 1.9 months0.41 (0.26–0.63)First FDA-approved oral SERD for ESR1-mut; benefit enriched with ≥ 12 months prior CDK4/6i (8.6 vs. 1.9 months)[18]
EMBER-3ImlunestrantPost-AI ± CDK4/6iImlunestrant monotherapy or
imlunestrant + abemaciclib
SOC ET (fulvestrant or exemestane)10.9 vs. 5.5 months (imlunestrant–abemaciclib vs. imlunestran)0.59 (0.47–0.74)mOS 34.5 vs. 23.1 months (HR 0.60) in ESR1-mut; combination with abemaciclib improved PFS regardless of ESR1 status[67]
SERENA-2 (phase 2,
non-registrational)
CamizestrantPost-ET progressionCamizestrant (75 mg or 150 mg)Fulvestrant6.3 vs. 2.2 months0.55 (0.33–0.91)Phase 2: both 75 mg and 150 mg doses superior to fulvestrant; supported dose selection for SERENA-6[16, 72]
VERITAC-2VepdegestrantPost-CDK4/6i progressionVepdegestrant 200 mg daily (oral)Fulvestrant 500 mg IM5.0 vs. 2.1 months0.58 (0.43–0.78)First phase 3 PROTAC ER degrader trial; primary endpoint met in ESR1-mut population only; ORR 18.6% vs. 4.0%; NDA submitted to FDA[68]
persevERAGiredestrant
+ palbociclib
Treatment-naïve, first-line;
ER+/HER2–– LA/MBC
Giredestrant + palbociclibLetrozole + palbociclibNot reportedNot reportedNegative trial; numerical but not statistically significant PFS improvement in ITT population; ESR1-mut subgroup data and full results pending presentationNCT04546009
Interception trials: ctDNA-guided switching ahead of progression
SERENA-6Camizestrant switch
(double-blind,
placebo-controlled)
ESR1 emergence during first-line therapy AI + CDK4/6i; no clinical/radiologic progressionCamizestrant 75 mg + CDK4/6i + placebo for AIContinue AI + CDK4/6i + placebo for camizestrant16.0 vs. 9.2 months0.44 (0.31–0.60)First global registrational ctDNA-guided switching trial; only double-blind, placebo-controlled interception trial; 56% relative reduction in progression/death risk[24]
PADA-1Fulvestrant switchESR1 emergence during first-line therapy AI + palbociclib; no clinical/radiologic progressionSwitch to fulvestrant + palbociclibContinue AI + palbociclib11.9 vs. 5.7 months (fulvestrant and palbociclib group vs. AI and palbociclib group)0.61 (0.43–0.86)Proof-of-concept for ctDNA-guided switching; first trial to demonstrate PFS benefit from early therapy switch guided by liquid biopsy[13]

AI: aromatase inhibitor; CDK4/6i: cyclin-dependent kinase 4 and 6 inhibitors; ctDNA: circulating tumor DNA; ER: estrogen receptor; ET: endocrine therapy; FDA: Food and Drug Administration; HER2: human epidermal growth factor receptor 2; HR: hazard ratio; IM: intramuscular; ITT: intent-to-treat; LA/MBC: locally advanced/metastatic breast cancer; mOS: median overall survival; NDA: new drug application; ORR: objective response rate; PFS: progression-free survival; PROTAC: proteolysis-targeting chimera; SERD: selective estrogen receptor degrader; SOC: standard of care.