From:  Cancer vaccines: advances, hurdles, and future directions

 Comparative overview of cancer vaccine modalities.

FeatureFully personalizedSemi-personalizedOff-the-shelf
Antigen sourceUnique neoantigens from each patient’s tumorShared neoepitopes (e.g., hotspot mutations, public neoantigens)Tumor-associated antigens (TAAs) are common across patients
HLA matchingPatient-specific HLA binding predictionsDesigned for frequent HLA alleles (e.g., HLA-A*02:01)Broad population, minimal HLA stratification
Manufacturing lead time6–12 weeks (custom synthesis, QC, formulation)1–3 weeks (batch-based pre-manufactured panels)Immediate (pre-made and stocked)
Production scaleIndividual (n = 1)Small-to-medium batches matched to subgroupsMass production possible
Cost per dose (estimated)$$$$ (high; $100k+ per patient)$$$$$ (moderate; depends on HLA/mutation match rates)$ (low; standardized production)
Regulatory pathwayComplex; often under IND or adaptive frameworksMore streamlined; batch-based approval possibleConventional biologics license application (BLA)
Example technologiesmRNA vaccines tailored to private neoantigensmRNA or peptide vaccines targeting KRAS G12D, TP53 R175H, etc.PROSTVAC, NY-ESO-1, HPV E6/E7 vaccines
Clinical flexibilityMaximal specificity; can target unique mutanomesBalances specificity and scalabilityLow specificity; limited by immune tolerance
Turnaround from biopsy to dosing~8–12 weeks~2–3 weeks0 weeks
ChallengesHigh cost, time, and individualized QA/QCHLA restrictions, partial personalizationPoor immunogenicity, immune tolerance

HLA: human leukocyte antigen; HPV: human papillomavirus; mRNA: messenger ribonucleic acid.