Primers sequence for HPV genotyping
| Genotypes | Sequence | Amplicon (bp) |
|---|---|---|
| HPV-16 F | 5’-AGAACTGCAATGTTTCAGGACCCACAG-3’ | 120 |
| HPV-16 R | 5’-TCTGCAACAAGACATACATCGACCGG-3’ | |
| HPV-18 F | 5’-TGAGGATCCAACACGGC-3’ | 120 |
| HPV-18 R | 5’-CCTCTGTAAGTTCCAATACTGTC-3’ |
F: forward primer; R: reverse primer. HPV: human papillomavirus
To the involved institutions, the patients, and those who provided and cared for the study patients.
RMRDL: Data curation, Formal analysis, Investigation, Methodology, Writing—original draft. DBB, CTGS, KGD, and WASJ: Formal analysis, Investigation, Methodology. PES and SFS: Visualization, Validation. NDA and CdO: Visualization, Writing—review & editing. LOR: Funding acquisition, Supervision, Resources, Writing—review & editing. RBdR: Resources, Conceptualization, Project administration. All authors read and approved the submitted version.
The authors declare that they have no conflicts of interest.
This study was approved by the Research Ethics Committee of the Clinical Hospital of Ribeirão Preto (University of São Paulo) under approval number 1.909.052/2016. All procedures involving human participants were performed by the ethical standards of the institutional and national research committees, as well as with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.
Written informed consent was obtained from all participants before inclusion in the study.
Not applicable.
The collected nonpersonal patient data and material transfer of this study are available after communication and agreement of the lead contact.
National Council for Scientific and Technological Development, CNPq, Research Productivity, grant number [310135/2022-2, 408576/2024-3] and INCT-UROGEN. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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