Table Info

Table 1.

Primer sequences for B2M and CD58 PCR

Name	Primer sequences (5'–3')	Location	PCR product size (bp)
Gene B2M
B2M1D	CAGACAGCAAACTCACCCAGT	Exon 1	451
B2M1R	CTTCCCCGAGATCCAGCCCT	Exon 1	451
B2M2Dx	CTTGACACCAAGTTAGCCCCA	Exon 2	510
B2M2Rx	GAACATTCCCTGACAATCCCA	Exon 2	510
Gene CD58
CD58D1	GGAGCCCTACTTCTGGCCGA	Exon 1	276
CD58R1	CCGTCCCCACCCGTCTCTGA	Exon 1	276
CD58D2x	GTGTCAGCAGTTTGTCAGCT	Exon 2	517
CD58R2x	CCCTGACAACAGGTAACATCT	Exon 2	517
CD58D3	GGAGTTTGTCTGCTCATCCT	Exon 3	450
CD58R3	GAACCTTGTGTTAGTCACCACA	Exon 3	450

D: forward primers; R: reverse primers. B2M: beta-2-microglobulin; PCR: polymerase chain reaction

Declarations

Author contributions

YKM: Conceptualization, Project administration, Data curation, Writing—review & editing. RRA: Investigation, Writing—original draft, Formal analysis, Visualization. NVR: Conceptualization, Investigation, Project administration, Data curation, Methodology, Resources, Writing—review & editing. BVB: Investigation, Data curation, Writing—review & editing. TVA: Investigation, Data curation, Writing—original draft. VLS: Methodology, Resources. IAS: Investigation, Data curation. TNO: Conceptualization, Writing—review & editing, Resources. RII: Investigation, Data curation, Writing—original draft. YAC: Methodology, Formal analysis, Visualization. AUM: Conceptualization. LEN: Investigation. SMK: Methodology, Formal analysis, Visualization. EEZ: Conceptualization. AMK: Conceptualization, Writing—review & editing, Resources. ABS: Conceptualization, Writing—review & editing, Supervision. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The present study has been accepted by the institutional ethical committee (National Medical Research Center for Hematology, Moscow, Russian Federation, protocol #165/30.06.2022). At this committee, we have approved the use of retrospective data from patients who received treatment before 2022 for our study. For patients who were included in the study and received treatment between 2007 and 2013, the study was conducted in accordance with the Helsinki Declaration of 1975, revised in 2008. As for the patients who received treatment after 2013, the study was conducted in accordance with the Helsinki Declaration of 1975, revised in 2013. All patients provided informed consent under National Medical Research Center for Hematology approved protocol allowing collection and analysis of data.

Consent to participate

Informed consent to participate in the study was obtained from all participants.

Consent to publication

Not applicable.

Availability of data and materials

The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.

Funding

This study was supported by RAKFOND (THE FOUNDATION FOR CANCER RESEARCH SUPPORT, Russia) grant [2.2020]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

Publisher’s note

Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.

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