RC: Conceptualization, Investigation, Writing—original draft, Writing—review & editing, Supervision. VC and LR: Investigation, Visualization, Writing—review & editing. All authors read and approved the submitted version.
Conflicts of interest
The authors declare that they have no conflicts of interest.
Ethical approval
The authors confirm that, in accordance with Italian law 211/2003, the research involved in this article is retrospective and does not require ethical approval. All patient data were collected anonymously in accordance with the Declaration of Helsinki and the study adhered to Good Clinical Practice guidelines.
Consent to participate
Patient consent was waived due to the retrospective and observational nature of the study.
Consent to publication
Not applicable.
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References
Gigon L, Fettrelet T, Yousefi S, Simon D, Simon HU. Eosinophils from A to Z.Allergy. 2023;78:1810–46. [DOI] [PubMed]
Hu G, Wang S, Zhong K, Xu F, Huang L, Chen W, et al. Tumor-associated tissue eosinophilia predicts favorable clinical outcome in solid tumors: a meta-analysis.BMC Cancer. 2020;20:454. [DOI] [PubMed] [PMC]
Ueki S, Melo RC, Ghiran I, Spencer LA, Dvorak AM, Weller PF. Eosinophil extracellular DNA trap cell death mediates lytic release of free secretion-competent eosinophil granules in humans.Blood. 2013;121:2074–83. [DOI] [PubMed] [PMC]
Uribe Echevarría L, Leimgruber C, García González J, Nevado A, Álvarez R, García LN, et al. Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease?Int J Chron Obstruct Pulmon Dis. 2017;12:885–96. [DOI] [PubMed] [PMC]
Barroso MV, Gropillo I, Detoni MAA, Thompson-Souza GA, Muniz VS, Vasconcelos CRI, et al. Structural and Signaling Events Driving Aspergillus fumigatus-Induced Human Eosinophil Extracellular Trap Release.Front Microbiol. 2021;12:633696. [DOI] [PubMed] [PMC]
Fukuchi M, Kamide Y, Ueki S, Miyabe Y, Konno Y, Oka N, et al. Eosinophil ETosis-Mediated Release of Galectin-10 in Eosinophilic Granulomatosis With Polyangiitis.Arthritis Rheumatol. 2021;73:1683–93. [DOI] [PubMed] [PMC]
Koike H, Nishi R, Furukawa S, Mouri N, Fukami Y, Iijima M, et al. In vivo visualization of eosinophil secretion in eosinophilic granulomatosis with polyangiitis: An ultrastructural study.Allergol Int. 2022;71:373–82. [DOI] [PubMed]
Neves VH, Palazzi C, Bonjour K, Ueki S, Weller PF, Melo RCN. In Vivo ETosis of Human Eosinophils: The Ultrastructural Signature Captured by TEM in Eosinophilic Diseases.Front Immunol. 2022;13:938691. [DOI] [PubMed] [PMC]
Hashimoto T, Ueki S, Kamide Y, Miyabe Y, Fukuchi M, Yokoyama Y, et al. Increased Circulating Cell-Free DNA in Eosinophilic Granulomatosis With Polyangiitis: Implications for Eosinophil Extracellular Traps and Immunothrombosis.Front Immunol. 2022;12:801897. [DOI] [PubMed] [PMC]
Neves VH, Palazzi C, Malta KK, Bonjour K, Kneip F, Dias FF, et al. Extracellular sombrero vesicles are hallmarks of eosinophilic cytolytic degranulation in tissue sites of human diseases.J Leukoc Biol. 2024;116:398–408. [DOI] [PubMed] [PMC]
Caruso R, Caruso V, Rigoli L. Ultrastructural Evidence of Eosinophil Clustering and ETosis in Association with Damage to Single Tumour Cells in a Case of Poorly Cohesive NOS Gastric Carcinoma.Eur J Case Rep Intern Med. 2023;10:004016. [DOI] [PubMed] [PMC]
Leonidas DD, Elbert BL, Zhou Z, Leffler H, Ackerman SJ, Acharya KR. Crystal structure of human Charcot-Leyden crystal protein, an eosinophil lysophospholipase, identifies it as a new member of the carbohydrate-binding family of galectins.Structure. 1995;3:1379–93. [DOI] [PubMed]
Ueki S, Miyabe Y, Yamamoto Y, Fukuchi M, Hirokawa M, Spencer LA, et al. Charcot-Leyden Crystals in Eosinophilic Inflammation: Active Cytolysis Leads to Crystal Formation.Curr Allergy Asthma Rep. 2019;19:35. [DOI] [PubMed] [PMC]
Ueki S, Tokunaga T, Melo RCN, Saito H, Honda K, Fukuchi M, et al. Charcot-Leyden crystal formation is closely associated with eosinophil extracellular trap cell death.Blood. 2018;132:2183–7. [DOI] [PubMed] [PMC]
Melo RCN, Wang H, Silva TP, Imoto Y, Fujieda S, Fukuchi M, et al. Galectin-10, the protein that forms Charcot-Leyden crystals, is not stored in granules but resides in the peripheral cytoplasm of human eosinophils.J Leukoc Biol. 2020;108:139–49. [DOI] [PubMed] [PMC]
Neves JS, Perez SA, Spencer LA, Melo RC, Reynolds L, Ghiran I, et al. Eosinophil granules function extracellularly as receptor-mediated secretory organelles.Proc Natl Acad Sci U S A. 2008;105:18478–83. [DOI] [PubMed] [PMC]
Muniz VS, Baptista-Dos-Reis R, Neves JS. Functional extracellular eosinophil granules: a bomb caught in a trap.Int Arch Allergy Immunol. 2013;162:276–82. [DOI] [PubMed]
Melo RCN, Weller PF. Contemporary understanding of the secretory granules in human eosinophils.J Leukoc Biol. 2018;104:85–93. [DOI] [PubMed] [PMC]
Legrand F, Driss V, Delbeke M, Loiseau S, Hermann E, Dombrowicz D, et al. Human eosinophils exert TNF-α and granzyme A-mediated tumoricidal activity toward colon carcinoma cells.J Immunol. 2010;185:7443–51. [DOI] [PubMed]
Gatault S, Delbeke M, Driss V, Sarazin A, Dendooven A, Kahn JE, et al. IL-18 Is Involved in Eosinophil-Mediated Tumoricidal Activity against a Colon Carcinoma Cell Line by Upregulating LFA-1 and ICAM-1.J Immunol. 2015;195:2483–92. [DOI] [PubMed]
Andreone S, Spadaro F, Buccione C, Mancini J, Tinari A, Sestili P, et al. IL-33 Promotes CD11b/CD18-Mediated Adhesion of Eosinophils to Cancer Cells and Synapse-Polarized Degranulation Leading to Tumor Cell Killing.Cancers (Basel). 2019;11:1664. [DOI] [PubMed] [PMC]
Mattei F, Andreone S, Marone G, Gambardella AR, Loffredo S, Varricchi G, et al. Eosinophils in the Tumor Microenvironment. In: Birbrair A, editor. Tumor Microenvironment: Hematopoietic Cells – Part B. Cham: Springer International Publishing; 2020. pp. 1–28. [DOI] [PubMed]
Li X, Ye Y, Peng K, Zeng Z, Chen L, Zeng Y. Histones: The critical players in innate immunity.Front Immunol. 2022;13:1030610. [DOI] [PubMed] [PMC]
Aoki A, Hirahara K, Kiuchi M, Nakayama T. Eosinophils: Cells known for over 140 years with broad and new functions.Allergol Int. 2021;70:3–8. [DOI] [PubMed]
Thompson-Souza GA, Vasconcelos CRI, Neves JS. Eosinophils: Focus on DNA extracellular traps.Life Sci. 2022;311:121191. [DOI] [PubMed]
