Exploration of Targeted Anti-tumor Therapy

Table 2. IC₅₀ values for the cytotoxic effects of 2AFH fractions on HeLa and MRC5-SV2 cells following 24 h and 48 h treatments (n = 3)

2AFH fractions	HeLa (IC₅₀, μg/mL)		MRC5-SV2 (IC₅₀, μg/mL)
2AFH fractions	24 h	48 h	24 h	48 h
2AFH25	60.9 ± 7.7	39.6 ± 7.2	41.8 ± 12.0	27.9 ± 4.3
2AFH40	25.5 ± 3.4	18.9 ± 0.4	24.6 ± 2.8	26.7 ± 4.7
2AFH50	31.3 ± 4.4	14.9 ± 0.8	26.4 ± 4.5	12.8 ± 1.0
2AFH60	24.6 ± 2.1	18.5^a	26.4 ± 4.7	15.9 ± 0.9
2AFH80	24.3^a	27.0 ± 6.9^b	22.6 ± 3.1^b	16.4 ± 0.6
2AFH100	83.2 ± 3.6	35.4 ± 1.2	47.5 ± 1.5	30.9 ± 3.6
2AFH100MeOH	ND	104.0 ± 15.2	ND	80.4 ± 11.2

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ND: not determined. ^a value is for n = 1, and ^b value is for n = 2

Declarations

Acknowledgments

The Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, UK is gratefully acknowledged for the access to instruments, resources, and training provided BEA while he conducted the research at the centre as a visiting PhD student between June and October 2019, hosted by AAF and supervised by AAF, SDS and DWS.

Author contributions

BEA: Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Validation, Visualization, Writing—original draft, Writing—review & editing. BO: Conceptualization, Supervision, Writing—review & editing. JBM: Conceptualization, Supervision, Writing—review & editing. DWS: Formal analysis, Investigation, Methodology, Supervision, Validation, Writing—review & editing. SDS: Methodology, Resources, Supervision, Validation, Writing—review & editing. AAF: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing—review & editing. All authors read and approved the manuscript.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

Not applicable.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

Datasets are available from the authors upon reasonable request. Contact tobitune@yahoo.com or A.A.Fatokun@ljmu.ac.uk.

Funding

The work described in this manuscript was partly supported by a Seed Corn Grant (2017) awarded to AAF by Liverpool John Moores University (LJMU). This funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

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