IC50 values for the cytotoxic effects of 2AFH fractions on HeLa and MRC5-SV2 cells following 24 h and 48 h treatments (n = 3)
2AFH fractions
HeLa (IC50, μg/mL)
MRC5-SV2 (IC50, μg/mL)
24 h
48 h
24 h
48 h
2AFH25
60.9 ± 7.7
39.6 ± 7.2
41.8 ± 12.0
27.9 ± 4.3
2AFH40
25.5 ± 3.4
18.9 ± 0.4
24.6 ± 2.8
26.7 ± 4.7
2AFH50
31.3 ± 4.4
14.9 ± 0.8
26.4 ± 4.5
12.8 ± 1.0
2AFH60
24.6 ± 2.1
18.5a
26.4 ± 4.7
15.9 ± 0.9
2AFH80
24.3a
27.0 ± 6.9b
22.6 ± 3.1b
16.4 ± 0.6
2AFH100
83.2 ± 3.6
35.4 ± 1.2
47.5 ± 1.5
30.9 ± 3.6
2AFH100MeOH
ND
104.0 ± 15.2
ND
80.4 ± 11.2
ND: not determined. a value is for n = 1, and b value is for n = 2
Declarations
Acknowledgments
The Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, UK is gratefully acknowledged for the access to instruments, resources, and training provided BEA while he conducted the research at the centre as a visiting PhD student between June and October 2019, hosted by AAF and supervised by AAF, SDS and DWS.
The work described in this manuscript was partly supported by a Seed Corn Grant (2017) awarded to AAF by Liverpool John Moores University (LJMU). This funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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