Cytotoxicity of 2A fractions: 2AFH, 2AFM and 2AFU against HeLa and MRC5-SV2 cells
2A Fractions
Cell line
ET (h)
Cell viability (negative control normalised to 100%)
IC50 (μg/mL)
10 μg/mL
50 μg/mL
100 μg/mL
200 μg/mL
2AFH
HeLa
48
72.3 ± 2.5***
48.7 ± 3.0***
39.0 ± 4.9***
32.7 ± 4.7***
59.4 ± 11.1
72
104.6 ± 3.3
59.6 ± 2.8***
40.3 ± 3.6***
31.2 ± 1.2***
84.0 ± 6.1
MRC5-SV2
48
80.4 ± 5.1*
55.3 ± 4.3***
42.1 ± 3.6***
33.3 ± 4.0***
63.2 ± 12.4
72
104.3 ± 3.9
49.4 ± 6.6***
30.3 ± 5.3***
21.5 ± 3.5***
60.4 ± 11.8
2AFM
HeLa
48
120.6 ± 4.3
120.8 ± 6.8
109.0 ± 2.4
48.2 ± 7.5***
> 700
72
106.7 ± 5.8
111.4 ± 2.0
78.9 ± 6.5
27.5 ± 3.0***
235.0 ± 17.8
MRC5-SV2
48
105.0 ± 3.5
115.4 ± 3.7
98.9 ± 5.4
46.6 ± 11.8***
> 500
72
136.3 ± 15.2
112.8 ± 1.1
100.5 ± 4.2
63.0 ± 3.1***
> 600
2AFU
HeLa
48
74.8 ± 2.0***
60.9 ± 2.7***
50.1 ± 4.1***
41.3 ± 4.3***
99.0 ± 14.2
72
79.6 ± 4.6**
63.0 ± 3.4***
49.5 ± 3.3***
30.2 ± 3.0***
87.0 ± 10.7
MRC5-SV2
48
74.3 ± 3.1***
62.1 ± 2.7***
54.5 ± 1.9***
43.8 ± 0.9***
109.0 ± 12.2
72
106.9 ± 4.4
76.7 ± 1.7*
56.5 ± 4.1***
38.5 ± 4.6***
146.0 ± 17.5
*P < 0.05, **P < 0.01, and ***P < 0.001 indicate a significant difference between the cell viability of treated cells and the cell viability of untreated cells of the negative control wells. ET: exposure time in hours
Declarations
Acknowledgments
The Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, UK is gratefully acknowledged for the access to instruments, resources, and training provided BEA while he conducted the research at the centre as a visiting PhD student between June and October 2019, hosted by AAF and supervised by AAF, SDS and DWS.
The work described in this manuscript was partly supported by a Seed Corn Grant (2017) awarded to AAF by Liverpool John Moores University (LJMU). This funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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