Table Info

Table 3.

Primer characteristics

Gene name/NCBI accession NR	Efficiency test mRNA	Amplicon size (bp)	Primer forward	Primer reverse
GAPDH/NM_001357943.2	2.00/universal RNA	115	GCTCTCTGCTCCTCCTGTTC	ACGACCAAATCCGTTGACTC
UBC/NM_021009.7	2.00/universal RNA	105	CTGATCAGCAGAGGTTGATCTTT	GACGGAGTACCAGGTGCAAG
HPRT1/NM_000194.3	1.98/universal RNA	94	TGACACTGGCAAAACAATGCA	GGTCCTTTTCACCAGCAAGCT
PGK1/NM_000291.4	2.00/universal RNA	181	GGACAATGGAGCCAAGTCGG	TGGGTTGGCACAGGCTTTCT
COL1A1/NM_000088.4	1.96/universal RNA	185	TGGTTCGTGACCGTGACCTC	CAGGTTGCAGCCTTGGTTGG
ZNF750/NM_024702.3	2.00/UDSCC5	83	GCACAGAATGCCTACCTGCC	AGGAGGGTCTCCGTTCACAAC
MAGEA6/NM_005363.5	2.00/universal RNA	182	TCGGGGATCCCAAGAAGCTG	AATGCGAGGTCCTCCACTGA
Ly6D/NM_003695.3	1.89/UDSCC5	77	GCCAGCTCTCGCTTCTGCAA	TCCGCACAGTCCTTCTTCACC
WNT5a/XM_011534089.2	2.00/universal RNA	66	AGCGGGTTCCTGAGTGAAT	CCCCTGACCCCTCTCTAGTT
FAT1/NM_005245.4	2.00/universal RNA	153	ATCCTGCAACCGGCTCTCTC	CGTGGTCATTCGTGTCGCTG
KRT5/NM_000424.4	2.00/universal RNA	91	GCAGATCAAGACCCTCAACAAT	CCACTTGGTGTCCAGAACCT
EGFR/NM_005228.5	2.00/universal RNA	200	TCCTCATTGCCCTCAACACAGT	GGCAGGGTTGTTGCTGAACC
PDPN/NM_198389.2	1.95/universal RNA	176	CTCAAACGTGGCCACCAGTC	GCCTTCCCGACATTTTTCGCA
CDH11/NM_001330576.2	2.00/universal RNA	195	ACACGGCCAATGGACCAAGA	TGCTACTCATGGGCGGGATG
CA12/NM_206925.3	2.00/universal RNA	173	AGTTTTCCGAAACCCCGTGC	GGATGATGCCTTGGGAGAAGGA
RBP1/NM_001130992.3	2.00/universal RNA	161	GCTCCAGTCACTCCCCGAAA	CACCGTCCTGCACGATCTCT
PLA2G4E/XM_011521238.2, XM_011521239.2, NM_001206670.1, XM_011521238.2	2.00/universal RNA UDSCC5	76, 143, 193, 197	GCCAGCTACATCACCGGTCT, TGTCAACTCCAGCTACCCGC, CAGAGCCCACAAACGGATGA, TGCAGAAGCGGAAGGTCGT	TGGAGGACCAGTCAGGGTCA, TGGGGAAGGGGATGTTCTGC, CAGCCTGCCGGACATTTTTCA, ACCGGTGATGTAGCTGGCAC

NR: number; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; UBC: ubiquitin C; HPRT1: hypoxanthine phosphoribosyltransferase 1; PGK1: phosphoglycerate kinase 1; COL1A1: collagen type I alpha 1 chain; ZNF750: zinc finger protein 750; MAGEA6: MAGE family member A6; Ly6D: lymphocyte antigen 6 family member D; WNT5a: Wnt family member 5a; FAT1: FAT atypical cadherin 1; KRT5: keratin 5; PDPN: podoplanin; CDH11: cadherin 11; CA12: carbonic anhydrase 12; RBP1: retinol binding protein 1; PLA2G4E: phospholipase A2 group IVE

Supplementary materials

The supplementary material for this article is available at: https://www.explorationpub.com/uploads/Article/file/1002143_sup_1.xlsx.

Declarations

Acknowledgments

Thanks are due to Ivonne Görlich, Katja Hasch, Gabriela Cudek and Monika Jerg for excellent technical assistance.

Author contributions

HAK and CB: Conceptualization, Methodology, Data Curation, Supervision, Project administration. HAK, MG, JMK and JE: Software. HAK, JMK, MG, LTH, AW, JE and CB: Validation, Investigation. JMK, MG, LTH, JE and AW: Formal analysis. HAK, TKH and CB: Resources. LTH and CB: Writing—original draft. HAK, JMK, MG, AW, JE, BW, SL and TKH: Writing—review and editing. JMK, JE and LTH: Visualization. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The study was approved by the local ethics committee of the Ulm University [90/15] and complies with the Declaration of Helsinki.

Consent to participate

Informed consent to participate in the study was obtained from all participants.

Consent to publication

Not applicable.

Availability of data and materials

RNA-seq data are available from Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156554) under Record ID GSE156554.

Funding

Not applicable.

Copyright

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