CellSave preservative tubes (Janssen) containing EDTA and a cell fixative;
not reported; pts: patients; n: number; N: nivolumab; P: pembrolizumab
Declarations
Author contributions
RD, FC and PMM conducted literature researches, wrote the paper and made the figure, FC, SS, EFG, and VD conducted literature researches and made the tables. FC and PMM revised the paper. All authors contributed to manuscript revision, read and approved the submitted version.
Conflicts of interest
The authors declare that they have no conflicts of interest.
Azevedo AS, Follain G, Patthabhiraman S, Harlepp S, Goetz JG.Metastasis of circulating tumor cells: favorable soil or suitable biomechanics, or both?Cell Adhes Migr. 2015;9:345–56. [DOI]
Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, Kaye J, et al. Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature.2015;523:177–82. [DOI] [PubMed] [PMC]
Schwarzenbach H, Hoon DSB, Pantel K.Cell-free nucleic acids as biomarkers in cancer patients. Nat Rev Cancer.2011;11:426–37. [DOI] [PubMed]
Ptashkin RN, Mandelker DL, Coombs CC, Bolton K, Yelskaya Z, Hyman DM, et al. Prevalence of clonal hematopoiesis mutations in tumor-only clinical genomic profiling of solid tumors. JAMA Oncol.2018;4:1589–93. [DOI] [PubMed] [PMC]
Snyder MW, Kircher M, Hill JA, Daza RM, Shendure J.Cell-free DNA comprises an in vivo nucleosome footprint that informs its tissues-of-origin. Cell.2016;164:57–68. [DOI] [PubMed] [PMC]
Szpechcinski A, Chorostowska-Wynimko J, Struniawski R, Kupis W, Rudzinski P, Langfort R, et al. Cell-free DNA levels in plasma of patients with non-small-cell lung cancer and inflammatory lung disease. Br J Cancer.2015;113:476–83. [DOI] [PubMed] [PMC]
Bardelli A, Pantel K.Liquid biopsies, what we do not know (yet). Cancer Cell.2017;31:172–9. [DOI] [PubMed]
Vitiello PP, De Falco V, Giunta EF, Ciardiello D, Cardone C, Vitale P, et al. Clinical practice use of liquid biopsy to identify RAS/BRAF mutations in patients with metastatic colorectal cancer (mCRC): a single institution experience. Cancers (Basel).2019;11:1504. [DOI]
Sclafani F, Chau I, Cunningham D, Hahne JC, Vlachogiannis G, Eltahir Z, et al. KRAS and BRAF mutations in circulating tumour DNA from locally advanced rectal cancer. Sci Rep.2018;8:1445. [DOI] [PubMed] [PMC]
Panagopoulou M, Karaglani M, Balgkouranidou I, Biziota E, Kakolyris S, Chatzaki E.Circulating cell-free DNA in breast cancer: size pro filing, levels, and methylation patterns lead to prognostic and predictive classifiers. Oncogene.2019;38:3387–401. [DOI] [PubMed]
Russo A, De Miguel Perez D, Gunasekaran M, Scilla K, Lapidus R, Cooper B, et al. Liquid biopsy tracking of lung tumor evolutions over time. Expert Rev Mol Diagn.2019;19:1099–108. [DOI] [PubMed]
Wu YL, Zhou C, Liam CK, Wu G, Liu X, Zhong Z, et al. First-line erlotinib versus gemcitabine/cisplatin in patients with advanced EGFR mutation-positive non-small-cell lung cancer: analyses from the phase III, randomized, open-label, ENSURE study. Ann Oncol.2015;26:1883–9. [DOI] [PubMed]
Rolfo C, Mack PC, Scagliotti GV, Baas P, Barlesi F, Bivona TG, et al. Liquid biopsy for advanced non-small cell lung cancer (NSCLC): a statement paper from the IASLC. J Thorac Oncol.2018;13:1248–68. [DOI] [PubMed]
Wu YL, Lee V, Liam CK, Lu S, Park K, Srimuninnimit V, et al. Clinical utility of a blood-based EGFR mutation test in patients receiving first-line erlotinib therapy in the ENSURE, FASTACT-2, and ASPIRATION studies. Lung Cancer.2018;126:1–8. [DOI] [PubMed]
Leighl NB, Page RD, Raymond VM, Daniel DB, Divers SG, Reckamp KL, et al. Clinical utility of comprehensive cell-free DNA analysis to identify genomic biomarkers in patients with newly diagnosed metastatic non-small cell lung cancer. Clin Cancer Res.2019;25:4691–700. [DOI] [PubMed]
Abbosh C, Birkbak NJ, Wilson GA, Jamal-Hanjani M, Constantin T, Salari R, et al. Phylogenetic ctDNA analysis depicts early stage lung cancer evolution. Nature.2018;545:446–51. [DOI]
Chaudhuri AA, Chabon JJ, Lovejoy AF, Newman AM, Stehr H, Azad TD, et al. Early detection of molecular residual disease in localized lung cancer by circulating tumor DNA profiling. Cancer Discov.2017;7:1394–403. [DOI] [PubMed] [PMC]
Gadgeel SM, Garassino MC, Esteban E, Speranza G, Felip E, Hochmair MJ, et al. KEYNOTE-189: updated OS and progression after the next line of therapy (PFS2) with pembrolizumab (pembro) plus chemo with pemetrexed and platinum vs. placebo plus chemo for metastatic nonsquamous NSCLC. J Clin Oncol.2019;37(15_suppl):9013. [DOI]
Planchard D, Popat S, Kerr K, Novello S, Smit EF, Faivre-Finn C, et al. Metastatic non-small cell lung cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol.2018;29:iv192–237.
Khattak MA, Reid A, Freeman J, Pereira M, McEvoy A, Lo J, et al. PD-L1 expression on circulating tumor cells may be predictive of response to pembrolizumab in advanced melanoma: results from a pilot study. Oncologist.2020;25:e520–7. [DOI] [PubMed] [PMC]
Seremet T, Jansen Y, Planken S, Njimi H, Delaunoy M, El Housni H, et al. Undetectable circulating tumor DNA (ctDNA) levels correlate with favorable outcome in metastatic melanoma patients treated with anti-PD1 therapy. J Transl Med.2019;17:303. [DOI] [PubMed] [PMC]
Strati A, Koutsodontis G, Papaxoinis G, Angelidis I, Zavridou M, Economopoulou P, et al. Prognostic significance of PD-L1 expression on circulating tumor cells in patients with head and neck squamous cell carcinoma. Ann Oncol.2017;28:1923–33. [DOI] [PubMed]
Lu T, Chen Y, Li J, Guo Q, Lin W, Zheng Y, et al. High soluble programmed death-ligand 1 predicts poor prognosis in patients with nasopharyngeal carcinoma. Onco Targets Ther.2020;13:1757–65. [DOI] [PubMed] [PMC]
Anantharaman A, Friedlander T, Lu D, Krupa R, Premasekharan G, Hough J, et al. Programmed death-ligand 1 (PD-L1) characterization of circulating tumor cells (CTCs) in muscle invasive and metastatic bladder cancer patients. BMC Cancer.2016;16:744. [DOI] [PubMed] [PMC]
Hong L, Negrao MV, Dibaj SS, Chen R, Reuben A, Bohac JM, et al. Programmed death ligand 1 heterogeneity and its impact on benefit from immune checkpoint inhibitors in non-small-cell lung cancer. J Thorac Oncol.2020;S1556-0864:30373–7. [DOI]
Yu H, Chen Z, Ballman KV, Watson MA, Govindan R, Lanc I, et al. Correlation of PD-L1 expression with tumor mutation burden and gene signatures for prognosis in early-stage squamous cell lung carcinoma. J Thorac Oncol.2019;14:25–36. [DOI] [PubMed] [PMC]
Davis AA, Patel VG.The role of PD-L1 expression as a predictive biomarker: an analysis of all US food and drug administration (FDA) approvals of immune checkpoint inhibitors. J Immunother Cancer.2019;7:278. [DOI] [PubMed] [PMC]
Haragan A, Field JK, Davies MPA, Escriu C, Gruver A, Gosney JR.Heterogeneity of PD-L1 expression in non-small cell lung cancer: implications for specimen sampling in predicting treatment response. Lung Cancer.2019;134:79–84. [DOI] [PubMed] [PMC]
Hiltermann TJN, Pore MM, Van den Berg A, Timens W, Boezen HM, Liesker JJW, et al. Circulating tumor cells in small-cell lung cancer: a redictive and prognostic factor. Ann Oncol.2012;23:2937–42. [DOI] [PubMed]
Hofman V, Ilie MI, Long E, Selva E, Bonnetaud C, Molina T, et al. Detection of circulating tumor cells as a prognostic factor in patients undergoing radical surgery for non-small-cell lung carcinoma: cmparison of the efficacy of the CellSearch AssayTM and the isolation by size of epithelial tumor cell method. Int J Cancer.2011;129:1651–60. [DOI] [PubMed]
De Giorgi U, Valero V, Rohren E, Mego M, Doyle G V, Miller MC, et al. Circulating tumor cells and bone metastases as detected by FDG-PET/CT in patients with metastatic breast cancer. Ann Oncol.2009;21:33–9. [DOI] [PubMed]
Zheng H, Zhan Y, Liu S, Lu J, Luo J, Feng J, et al. The roles of tumor-derived exosomes in non-small cell lung cancer and their clinical implications. J Exp Clin Cancer Res.2018;37:226. [DOI] [PubMed] [PMC]
De Wit S, Rossi E, Weber S, Tamminga M, Manicone M, Swennenhuis JF, et al. Single tube liquid biopsy for advanced non-small cell lung cancer. Int J Cancer.2019;144:3127–37. [DOI] [PubMed]
Tamminga M, De Wit S, Hiltermann TJN, Timens W, Schuuring E, Terstappen LWMM, et al. Circulating tumor cells in advanced non-small cell lung cancer patients are associated with worse tumor response to checkpoint inhibitors. J Immunother Cancer.2019;7:173. [DOI] [PubMed] [PMC]
Shin H, Oh S, Hong S, Kang M, Kang D, Ji Y, et al. Early-stage lung cancer diagnosis by deep learning-based spectroscopic analysis of circulating exosomes. ACS Nano.2020;4:5435–44. [DOI]
Boffa DJ, Graf RP, Salazar MC, Hoag J, Lu D, Krupa R, et al. Cellular expression of PD-L1 in the peripheral blood of lung cancer patients is associated with worse survival. Cancer Epidemiol Biomarkers Prev.2017;26:1139–145. [DOI] [PubMed] [PMC]
Nicolazzo C, Raimondi C, Mancini M, Caponnetto S, Gradilone A, Gandini O, et al. Monitoring PD-L1 positive circulating tumor cells in non-small cell lung cancer patients treated with the PD-1 inhibitor Nivolumab. Sci Rep.2016;6:31726. [DOI] [PubMed] [PMC]
Kloten V, Lampignano R, Krahn T, Schlange T.Circulating tumor cell PD-L1 expression as biomarker for therapeutic efficacy of immune checkpoint inhibition in NSCLC. Cells.2019;8:809. [DOI]
Guibert N, Delaunay M, Lusque A, Boubekeur N, Rouquette I, Clermont E, et al. PD-L1 expression in circulating tumor cells of advanced non-small cell lung cancer patients treated with nivolumab. Lung Cancer.2018;120:108–12. [DOI] [PubMed]
Kulasinghe A, Kapeleris J, Kimberley R, Mattarollo SR, Thompson EW, Thiery JP, et al. The prognostic significance of circulating tumor cells in head and neck and non-small-cell lung cancer. Cancer Med.2018;7:5910–9. [DOI] [PubMed] [PMC]
Dhar M, Wong J, Che J, Matsumoto M, Grogan T, Elashoff D, et al. Evaluation of PD-L1 expression on vortex-isolated circulating tumor cells in metastatic lung cancer. Sci Rep.2018;8:2592. [DOI] [PubMed] [PMC]
Nielsen C, Ohm-Laursen L, Barington T, Husby S, Lillevang ST.Alternative splice variants of the human PD-1 gene. Cell Immunol.2005;235:109–16. [DOI] [PubMed]
He XH, Xu LH, Liu Y.Identification of a novel splice variant of human PD-L1 mRNA encoding an isoform-lacking Igv-like domain. Acta Pharmacol Sin.2005;26:462–8. [DOI] [PubMed]
Zhang J, Gao J, Li Y, Nie J, Dai L, Hu W, et al. Circulating PD-L1 in NSCLC patients and the correlation between the level of PD-L1 expression and the clinical characteristics. Thorac Cancer.2015;6:534–8. [DOI] [PubMed] [PMC]
Okuma Y, Hosomi Y, Nakahara Y, Watanabe K, Sagawa Y, Homma S.High plasma levels of soluble programmed cell death ligand 1 are prognostic for reduced survival in advanced lung cancer. Lung Cancer.2017;104:1–6. [DOI] [PubMed]
Okuma Y, Wakui H, Utsumi H, Sagawa Y, Hosomi Y, Kuwano K, et al. Soluble programmed cell death ligand 1 as a novel biomarker for nivolumab therapy for non-small-cell lung cancer. Clin Lung Cancer.2018;19:410–7.e1. [DOI] [PubMed]
Costantini A, Julie C, Dumenil C, Hélias-Rodzewicz Z, Tisserand J, Dumoulin J, et al. Predictive role of plasmatic biomarkers in advanced non-small cell lung cancer treated by nivolumab. Oncoimmunology.2018;7:e1452581. [DOI] [PubMed] [PMC]
Liontos M, Anastasiou I, Bamias A, Dimopoulos MA.DNA damage, tumor mutational load and their impact on immune responses against cancer. Ann Transl Med.2016;4:264. [DOI] [PubMed] [PMC]
Anagnostou V, Smith KN, Forde PM, Niknafs N, Bhattacharya R, White J, et al. Evolution of neoantigen landscape during immune checkpoint blockade in non-small cell lung cancer. Cancer Discov.2017;7:264–76. [DOI] [PubMed] [PMC]
Rizvi H, Sanchez-Vega F, La K, Chatila W, Jonsson P, Halpenny D, et al. Molecular determinants of response to anti-programmed cell death (PD)-1 and anti-programmed death-ligand 1 (PD-L1) blockade in patients with non-small-cell lung cancer profiled with targeted next-generation sequencing. J Clin Oncol.2018;36:633–41. [DOI] [PubMed] [PMC]
Rizvi NA, Hellmann MD, Snyder A, Kvistborg P, Makarov V, Havel JJ, et al. Cancer immunology. Mutational landscape determines sensitivity to PD-1 blockade in non-small cell lung cancer. Science.2015;348:124–8. [DOI] [PubMed] [PMC]
Fenizia F, Pasquale R, Roma C, Bergantino F, Iannaccone A, Normanno N.Measuring tumor mutation burden in non-small cell lung cancer: tissue versus liquid biopsy. Transl Lung Cancer Res.2018;7:668–77. [DOI] [PubMed] [PMC]
Wang Z, Duan J, Cai S, Han M, Dong H, Zhao J, et al. Assessment of blood tumor mutational burden as a potential biomarker for immunotherapy in patients with non-small cell lung cancer with use of a next-generation sequencing cancer gene panel. JAMA Oncol.2019;5:696–702. [DOI] [PubMed] [PMC]
Socinski M, Velcheti V, Mekhail T, Chae YK, Leal TA, Dowell JE, et al. Final efficacy results from B-F1RST, a prospective phase II trial evaluating blood-based tumour mutational burden (bTMB) as a predictive biomarker for atezolizumab (atezo) in 1L non-small cell lung cancer (NSCLC). Ann Oncol.2019;30:v919–20.
Gandara DR, Paul SM, Kowanetz M, Schleifman E, Zou W, Li Y, et al. Blood-based tumor mutational burden as a predictor of clinical benefit in non-small-cell lung cancer patients treated with atezolizumab. Nat Med.2018;24:1441–8. [DOI] [PubMed]
Rizvi NA, Cho BC, Reinmuth N, Lee KH, Luft A, Ahn MJ, et al. Blood tumor mutational burden (bTMB) and tumor PD-L1 as predictive biomarkers of survival in MYSTIC: first-lie durvalumab (D) ± tremelimumab (T) versus chemotherapy (CT) in metastatic (m) NSCLC. J Clin Oncol.2019;37(15_suppl):9016. [DOI]
Snyder A, Morrissey MP, Hellmann MD.Use of circulating tumor DNA for cancer immunotherapy. Clin Cancer Res.2019;25:6909–15. [DOI] [PubMed]
George J, Lim JS, Jang SJ, Cun Y, Ozretić L, Kong G, et al. Comprehensive genomic profiles of small cell lung cancer. Nature.2015;524:47–53. [DOI] [PubMed] [PMC]
Byers LA, Rudin CM.Small cell lung cancer: where do we go from here?Cancer. 2015;121:664–72. [DOI] [PubMed] [PMC]
Della Corte CM, Gay CM, Byers LA.Beyond chemotherapy: emerging biomarkers and therapies as small cell lung cancer enters the immune checkpoint era. Cancer.2019;125:496–8. [DOI] [PubMed] [PMC]
Horn L, Mansfield AS, Szczȩsna A, Havel L, Krzakowski M, Hochmair MJ, et al. First-line atezolizumab plus chemotherapy in extensive-stage small-cell lung cancer. N Engl J Med.2018;379:2220–9. [DOI] [PubMed]
Antonia SJ, López-Martin JA, Bendell J, Ott PA, Taylor M, Eder JP, et al. Nivolumab alone and nivolumab plus ipilimumab in recurrent small-cell lung cancer (CheckMate 032): a multicentre, open-label, phase 1/2 trial. Lancet Oncol.2016;17:883–95. [DOI] [PubMed]
Hellmann MD, Callahan MK, Awad MM, Calvo E, Ascierto PA, Atmaca A, et al. Tumor mutational burden and efficacy of nivolumab monotherapy and in combination with ipilimumab in small-cell lung cancer. Cancer Cell.2018;33:853–61.e4. [DOI] [PubMed] [PMC]
Bedin C, Enzo MV, Del Bianco P, Pucciarelli S, Nitti D, Agostini M.Diagnostic and prognostic role of cell-free DNA testing for colorectal cancer patients. Int J Cancer.2017;140:1888–98. [DOI] [PubMed]
Arko-Boham B, Aryee NA, Blay RM, Owusu EDA, Tagoe EA, Doris Shackie ES, et al. Circulating cell-free DNA integrity as a diagnostic and prognostic marker for breast and prostate cancers. Cancer Genet.2019;235–236:65–71. [DOI]
Yu Z, Qin S, Wang H.Alter circulating cell-free DNA variables in plasma of ovarian cancer patients. J Obstet Gynaecol Res.2019;45:2237–42. [DOI] [PubMed]
Vizza E, Corrado G, De Angeli M, Carosi M, Mancini E, Baiocco E, et al. Serum DNA integrity index as a potential molecular biomarker in endometrial cancer. J Exp Clin Cancer Res.2018;37:16. [DOI] [PubMed] [PMC]
Kitahara M, Hazama S, Tsunedomi R, Takenouchi H, Kanekiyo S, Inoue Y, et al. Prediction of the efficacy of immunotherapy by measuring the integrity of cell-free DNA in plasma in colorectal cancer. Cancer Sci.2016;107:1825–9. [DOI] [PubMed] [PMC]
Soliman SES, Alhanafy AM, Habib MSE, Hagag M, Ibrahem RAL.Serum circulating cell free DNA as potential diagnostic and prognostic biomarker in non small cell lung cancer. Biochem Biophys Rep.2018;15:45–51. [DOI] [PubMed] [PMC]
Leng S, Zheng J, Jin Y, Zhang H, Zhu Y, Wu J, et al. Plasma cell-free DNA level and its integrity as biomarkers to distinguish non-small cell lung cancer from tuberculosis. Clin Chim Acta.2018;477:160–5. [DOI] [PubMed]
Szpechcinski A, Rudzinski P, Kupis W, Langfort R, Orlowski T, Chorostowska-Wynimko J.Plasma cell-free DNA levels and integrity in patients with chest radiological findings: NSCLC versus benign lung nodules. Cancer Lett.2016;374:202–7. [DOI] [PubMed]
Hofman P, Heeke S, Alix-Panabières C, Pantel K.Liquid biopsy in the era of immuno-oncology: is it ready for prime-time use for cancer patients?Ann Oncol. 2019;30:1448–59. [DOI] [PubMed]
Iijima Y, Hirotsu Y, Amemiya K, Ooka Y, Mochizuki H, Oyama T, et al. Very early response of circulating tumour-derived DNA in plasma predicts efficacy of nivolumab treatment in patients with non-small cell lung cancer. Eur J Cancer.2017;86:349–57. [DOI] [PubMed]
Goldberg SB, Narayan A, Kole AJ, Decker RH, Teysir J, Carriero NJ, et al. Early assessment of lung cancer immunotherapy response via circulating tumor DNA. Clin Cancer Res.2018;24:1872–80. [DOI] [PubMed] [PMC]
Kim ES, Velcheti V, Mekhail T, Leal TA, Dowell JE, Tsai ML, et al. Primary efficacy results from B-F1RST, a prospective phase II trial evaluating blood-based tumour mutational burden (bTMB) as a predictive biomarker for atezolizumab (atezo) in 1L non-small cell lung cancer (NSCLC). Ann Oncol.2018;29:viii744.
Gettinger S, Horn L, Jackman D, Spigel D, Antonia S, Hellmann M, et al. Five-year follow-up of nivolumab in previously treated advanced non-small-cell lung cancer: results from the CA209-003 study. J Clin Oncol.2018;36:1675–84. [DOI] [PubMed]
Hellmann MD, Nabet BY, Rizvi H, Chaudhuri AA, Wells DK, Dunphy MPS, et al. Circulating tumor DNA analysis to assess risk of progression after long-term response to PD-(L)1 blockade in NSCLC. Clin Cancer Res.2020;Online ahead of print.[DOI]
Lee JH, Long GV, Menzies AM, Lo S, Guminski A, Whitbourne K, et al. Association between circulating tumor DNA and pseudoprogression in patients with metastatic melanoma treated with anti-programmed cell death 1 antibodies. JAMA Oncol.2018;4:717–21. [DOI] [PubMed] [PMC]
Lipson EJ, Velculescu VE, Pritchard TS, Sausen M, Pardoll DM, Topalian SL, et al. Circulating tumor DNA analysis as a real-time method for monitoring tumor burden in melanoma patients undergoing treatment with immune checkpoint blockade. J Immunother Cancer.2014;2:42. [DOI] [PubMed] [PMC]
Guibert N, Mazieres J, Delaunay M, Casanova A, Farella M, Keller L, et al. Monitoring of KRAS-mutated ctDNA to discriminate pseudoprogression from true progression during anti-PD-1 treatment of lung adenocarcinoma. Oncotarget.2017;8:38056–60. [DOI] [PubMed] [PMC]
Sanmamed MF, Perez-Gracia JL, Schalper KA, Fusco JP, Gonzalez A, Rodriguez-Ruiz ME, et al. Changes in serum interleukin-8 (IL-8) levels reflect and predict response to anti-PD-1 treatment in melanoma and non-small-cell lung cancer patients. Ann Oncol.2017;28:1988–95. [DOI] [PubMed] [PMC]
Sanmamed MF, Carranza-Rua O, Alfaro C, Oñate C, Martín-Algarra S, Perez G, et al. Serum interleukin-8 reflects tumor burden and treatment response across malignancies of multiple tissue origins. Clin Cancer Res.2014;20:5697–707. [DOI] [PubMed]
Gonzalez-Aparicio M, Alfaro C.Influence of interleukin-8 and neutrophil extracellular trap (NET) formation in the tumor microenvironment: is there a pathogenic role?J Immunol Res. 2019;2019:6252138. [DOI] [PubMed] [PMC]
Durgeau A, Virk Y, Corgnac S, Mami-Chouaib F.Recent advances in targeting CD8 T-Cell immunity for more effective cancer immunotherapy. Front Immunol.2018;9:14. [DOI] [PubMed] [PMC]
Della Corte CM, Barra G, Ciaramella V, Di Liello R, Vicidomini G, Zappavigna S, et al. Antitumor activity of dual blockade of PD-L1 and MEK in NSCLC patients derived three-dimensional spheroid cultures. J Exp Clin Cancer Res.2019;38:253. [DOI] [PubMed] [PMC]
Di Liello R, Ciaramella V, Barra G, Venditti M, Della Corte CM, Papaccio F, et al. Ex vivo lung cancer spheroids resemble treatment response of a patient with NSCLC to chemotherapy and immunotherapy: case report and translational study. ESMO Open.2019;4:e000536. [DOI] [PubMed] [PMC]
Jia W, Gao Q, Han A, Zhu H, Yu J.The potential mechanism, recognition and clinical significance of tumor pseudoprogression after immunotherapy. Cancer Biol Med.2019;16:655–70. [DOI] [PubMed] [PMC]
Viscardi G, Di Liello R, Morgillo F.How I treat malignant pleural mesothelioma. ESMO Open.2020;4(Suppl 2):e000669. [DOI] [PubMed] [PMC]
Cavallari I, Urso L, Sharova E, Pasello G, Ciminale V.Liquid biopsy in malignant pleural mesothelioma: state of the art, pitfalls, and perspectives. Front Oncol.2019;9:740. [DOI] [PubMed] [PMC]
Udall M, Rizzo M, Kenny J, Doherty J, Dahm S, Robbins P, et al. PD-L1 diagnostic tests: a systematic literature review of scoring algorithms and test-validation metrics. Diagn Pathol.2018;13:12. [DOI] [PubMed] [PMC]
Parikh K, Huether R, White K, Hoskinson D, Dong H, Adjei AA, et al. Overestimation of tumor mutational burden (TMB) using algorithms compared to germline subtraction. J Clin Oncol.2019;37(15_suppl):2621. [DOI]
Merino DM, McShane L, Butler M, Funari VA, Hellmann MD, Chaudhary R, et al. TMB standardization by alignment to reference standards: phase II of the Friends of Cancer Research TMB Harmonization Project. J Clin Oncol.2019;37(15_suppl):2624. [DOI]
